{"code":"SASDE72","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties.","publication":{"title":"A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties.","author_list":"Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ","journal":"Sci Rep","doi":"10.1038/s41598-019-44385-y","pmid":"31138855","published_date":"2019 May 28"},"status":"released","submitted_date":"2018-07-30","released_date":"2019-05-28"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDE72.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDE72.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE72_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE72_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE72_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE72_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDE72.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Endoribonuclease E","short_name":"RNase E","sequence":"HHHHHHHHHHSSGHIEGRHMKRILINSKSGEETRIATLDNGKLIDLDIESVDREQKKANIYKGYISRIEPSLNAIFVNYGEEKNGFLPFKEVSEYYLKDVPNGENIAPLLSEGQELIVQIDKEERGDKGAALTTFITLAGSYMVLLPNNPEGGGISRRVEGEDRERLKKYLKELNIPKNMSVIARTACVECSFEELKHDFDTLVELWGSISQAYHRIKKPALLHKESDIIVRTVRDHLKEDVKEIIVDSKECFEDVKRQLSLLRQSFDINKVKLYNEELPLFAQFGIDQQIENAYKREIRLPSGGSIVIDTTEALVAIDVNSSRANKAEDVETTAFKTNLEAAEEVARQLRIRDLGGLVIVDFIDMSFYPNRKQVEEKLMEALQQDRARIQMSRISKLGLVEISRQRLSSSINESVMQKCPRCEGHGFIKTTQATALTILRKIRAEAIKEDTNEIRVQVPVDIAAYILNEKRDSIVEIERISQVKVMIIPNFNMESPKFQMQRIWGTSYKSNRTSSELIEDVYNIEIPKAGKKKIAAVDLNQAIENQAASNTQEQKPQTDAQ","organism":"Francisella tularensis","uniprot_code":"Q5NFK7","uniprot_range_first":1,"uniprot_range_last":543,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MKRILINSKSGEETRIATLDNGKLIDLDIESVDREQKKANIYKGYISRIEPSLNAIFVNY\nGEEKNGFLPFKEVSEYYLKDVPNGENIAPLLSEGQELIVQIDKEERGDKGAALTTFITLA\nGSYMVLLPNNPEGGGISRRVEGEDRERLKKYLKELNIPKNMSVIARTACVECSFEELKHD\nFDTLVELWGSISQAYHRIKKPALLHKESDIIVRTVRDHLKEDVKEIIVDSKECFEDVKRQ\nLSLLRQSFDINKVKLYNEELPLFAQFGIDQQIENAYKREIRLPSGGSIVIDTTEALVAID\nVNSSRANKAEDVETTAFKTNLEAAEEVARQLRIRDLGGLVIVDFIDMSFYPNRKQVEEKL\nMEALQQDRARIQMSRISKLGLVEISRQRLSSSINESVMQKCPRCEGHGFIKTTQATALTI\nLRKIRAEAIKEDTNEIRVQVPVDIAAYILNEKRDSIVEIERISQVKVMIIPNFNMESPKF\nQMQRIWGTSYKSNRTSSELIEDVYNIEIPKAGKKKIAAVDLNQAIENQAASNTQEQKPQT\nDAQVDIVTQNDKKQDDVTQQAKKKGFFARLFSGIFATDSKAAQQANNSAQKQQNKEQNFQ\nRQQQHNKAQKDNNQRNERNNSNNKNERNKNRNNKGAKPQIAQENKPQKDNSQRSDLNANG\nNSNNDKNERNKNKSNYDKFDKKSNNRNSRSNNLNLNNAEEVIDITQLKYQNQTAIAKTDN\nIKKVISKNPDEFISVMVKDVLENYDSLKDDGATKIATQEKIKTNKYLKFDTVSVDTEIAL\nLNEVAKSNQTVGVEQITAETEESLSIVDKVDDITIPEVVVEQEKPAKVVKETNQTSEAKP\nKKEKQTTTKNNKKEKPAEKEMKQNQAKPEYINYSPAIDFESQALI","mw":64.088,"total_mw":256.352,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Ribonuclease E from Francisella tularensis","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Portsmouth, UK","full_name":"University of Portsmouth","webpage":"https://www.port.ac.uk"}],"contributor_name":"A","contributor_surname":"Callaghan","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2017-02-11","storage_temperature":21.0,"cell_temperature":15.0,"exposure_time":3.0,"number_of_frames":640,"wavelength":0.1,"sample_detector_distance":4.014,"concentration_min":null,"concentration_max":6.41,"sample_volume":null,"flow_rate":null,"s_min":0.04,"s_max":4.42,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Ribonuclease E from Francisella tularensis in 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH 8 were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.41 mg/ml was measured at 15°C. 640 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":290.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":306.0,"porod_mw_error":null,"pddf_i0":0.02788,"pddf_i0_error":null,"guinier_i0":0.02792,"guinier_i0_error":6e-05,"pddf_rg":5.07,"pddf_rg_error":null,"guinier_rg":5.084,"guinier_rg_error":0.02,"pddf_dmax":17.23,"pddf_dmax_error":null,"porod_volume":491.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":13,"guinier_point_last":90,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDE72_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}