{"code":"SASDE83","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.","publication":{"title":"Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.","author_list":"Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA","journal":"Proc Natl Acad Sci U S A","doi":"10.1073/pnas.1704692114","pmid":"28716919","published_date":"2017 Aug 1"},"status":"released","submitted_date":"2018-08-16","released_date":"2018-08-18"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDE83.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDE83.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE83_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE83_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDE83_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDE83_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDE83.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Cold shock-like protein","short_name":"CSP-488/594","sequence":"> U=p-acetylphenylalanine. C was labeled with Alexa Fluor 594 C5 maleimide. U was labeled with Alexa Fluor 488 C5 hydroxylamine\r\nACGKVKFFDSKKGYGFITKDEGGDVFVHFSAIEMEGFKTLKEGQVVEFEIQEGKKGGQUA","organism":"Thermotoga maritima","uniprot_code":"O54310","uniprot_range_first":3,"uniprot_range_last":58,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MRGKVKWFDSKKGYGFITKDEGGDVFVHWSAIEMEGFKTLKEGQVVEFEIQEGKKGPQAA\nHVKVVE","mw":6.628,"total_mw":6.628,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Alexa Fluor™ 594 C5 Maleimide","short_name":"Alexa594","sequence":null,"organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"other","uniprot_sequence":null,"mw":0.886,"total_mw":0.886,"number_molecules":1,"complex_state":null,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Alexa Fluor™ 488 C5 Hydroxylamine","short_name":"Alexa488","sequence":null,"organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"other","uniprot_sequence":null,"mw":0.7,"total_mw":0.7,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"PBS, 10 mM DTT, 6 M urea, 0.3 M KCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Labeled cold shock protein (CSP-Alexa488/Alexa594) with denaturant","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Prague, Czech Republic","full_name":"Institute of Biotechnology CAS v.v.i.","webpage":"http://www.ibt.cas.cz/"}],"contributor_name":"Gustavo","contributor_surname":"Fuertes Vives","orcid":"https://orcid.org/0000-0002-8564-8644"}],"concentration_method":null,"concentration_unit":null,"date":"2013-06-15","storage_temperature":null,"cell_temperature":23.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.1,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.077,"s_max":4.583,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).","experiment_description":null,"tags":["idp"],"intensity_unit":"arbitrary","experimental_mw":7.3,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":358.6,"pddf_i0_error":2.1,"guinier_i0":355.4,"guinier_i0_error":1.7,"pddf_rg":2.309,"pddf_rg_error":0.02,"guinier_rg":2.2,"guinier_rg_error":0.06,"pddf_dmax":8.27,"pddf_dmax_error":null,"porod_volume":8.72,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":35,"guinier_point_last":196,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDE83_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}