{"code":"SASDEA8","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"Full length of DnaG primase from Bacillus subtilis","publication":null,"status":"released","submitted_date":"2018-11-21","released_date":"2021-09-20"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEA8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEA8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEA8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEA8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEA8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEA8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEA8.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Shanghai Synchrotron Radiation Facility (SSRF)","city":"Shanghai","country":"China","beamline_name":"BL19U2","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"DNA primase","short_name":null,"sequence":"MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFH\r\nCFGCGAGGNVFSFLRQMEGYSFAESVSHLADKYQIDFPDDITVHSGARPESSGEQKMAE\r\nAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQIGYALDSWDFITKFLVKRGF\r\nSEAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPET\r\nPLFHKSKLLYNFYKARLHIRKQERAVLFEGFADVISAVSSDVKESIATMGTSLTDDHVKI\r\nLRRNVEEIILCYDSDKAGYEATLKASELLQKKGCKVRVAMIPDGLDPDDYIKKFGGEKFK\r\nNDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEISTLSGSLEQEVYVKQLASE\r\nFSLSQESLTEQLSVFSKQNKPADNSGETKTRRAHLTTKARQKRLRPAYENAERLLLAHML\r\nRDRSVIKKVIDRVGFQFNIDEHRALAAYLYAFYEEGAELTPQHLMARVTDDHISQLLSDI\r\nLMLQVNQELSEAELSDYVKKVLNQRNWSMIKEKEAERAEAERQKDFLRAASLAQEIVTLN\r\nRSLK","organism":"Bacillus subtilis","uniprot_code":"P05096","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFH\nCFGCGAGGNVFSFLRQMEGYSFAESVSHLADKYQIDFPDDITVHSGARPESSGEQKMAEA\nHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQIGYALDSWDFITKFLVKRGFS\nEAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETP\nLFHKSKLLYNFYKARLHIRKQERAVLFEGFADVISAVSSDVKESIATMGTSLTDDHVKIL\nRRNVEEIILCYDSDKAGYEATLKASELLQKKGCKVRVAMIPDGLDPDDYIKKFGGEKFKN\nDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEISTLSGSLEQEVYVKQLASEF\nSLSQESLTEQLSVFSKQNKPADNSGETKTRRAHLTTKARQKRLRPAYENAERLLLAHMLR\nDRSVIKKVIDRVGFQFNIDEHRALAAYLYAFYEEGAELTPQHLMARVTDDHISQLLSDIL\nMLQVNQELSEAELSDYVKKVLNQRNWSMIKEKEAERAEAERQKDFLRAASLAQEIVTLNR\nSLK","mw":68.735,"total_mw":68.735,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris-HCl,100 mM NaCl","concentration_unit":null,"comment":"25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM DTT","additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Full length of DnaG (DNA Primase) from Bacillus subtilis","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Chengdu, China","full_name":"Chengdu Institute of Biology, Chinese academy of Sciences","webpage":"http://english.cib.cas.cn/"}],"contributor_name":"Zhongchuan","contributor_surname":"Liu","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2018-10-01","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":1.0,"number_of_frames":20,"wavelength":0.154,"sample_detector_distance":2.0,"concentration_min":1.0,"concentration_max":7.0,"sample_volume":null,"flow_rate":null,"s_min":0.084,"s_max":3.954,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEA8_fit1_model1_img.png","software":"CORAL","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEA8_fit1_model1.pdb","model_mw":68.46,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":240}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEA8_fit1_fixed_fit_img.png","software":"CORAL","chi_square_value":0.95,"p_value":0.554373,"fit_residual_plot":"SASDEA8_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEA8_fit1.fit","fit_log":null,"software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEA8_fit2_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEA8_fit2_model1.pdb","model_mw":82.8,"bead_radius":5.0,"log":null,"symmetry":null,"comment":null,"user":240}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEA8_fit2_fixed_fit_img.png","software":"DAMMIF","chi_square_value":1.176,"p_value":0.050515,"fit_residual_plot":"SASDEA8_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEA8_fit2.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of full length of DnaG (DNA Primase) from Bacillus subtilis in 25 mM Tris-HCl,100 mM NaCl, pH 8 were collected on the NCPSS BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Dectris Pilatus 1M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.154 nm in the momentum transfer range of  0.2  < s < 4 1/nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 7 mg/ml were measured at 10°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"arbitrary","experimental_mw":70.0,"experimental_mw_error":null,"guinier_i0_mw":70.0,"guinier_i0_mw_error":2.0,"porod_mw":74.0,"porod_mw_error":null,"pddf_i0":90.9,"pddf_i0_error":null,"guinier_i0":89.22,"guinier_i0_error":0.24,"pddf_rg":3.94,"pddf_rg_error":null,"guinier_rg":3.74,"guinier_rg_error":0.1,"pddf_dmax":14.5,"pddf_dmax_error":null,"porod_volume":119.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":7,"guinier_point_last":56,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEA8_datloglog_img.png","symmetry":null,"last_modified":"2021-09-20T13:42:19.789088+02:00","bragg_peak":[]}