{"code":"SASDEA9","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin in the green alga <i>Chlamydomonas reinhardtii</i>.","publication":{"title":"Calcium sensing via EF-hand 4 enables thioredoxin activity in the sensor-responder protein calredoxin in the green alga <i>Chlamydomonas reinhardtii</i>.","author_list":"Charoenwattanasatien R, Zinzius K, Scholz M, Wicke S, Tanaka H, Brandenburg JS, Marchetti GM, Ikegami T, Matsumoto T, Oda T, Sato M, Hippler M, Kurisu G","journal":"J Biol Chem","doi":"10.1074/jbc.RA119.008735","pmid":"31776187","published_date":"2019 Nov 27"},"status":"released","submitted_date":"2018-12-27","released_date":"2019-12-17"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEA9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEA9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEA9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEA9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEA9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEA9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEA9.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"Structural Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University","city":"Yokohama","country":"Japan","beamline_name":"Rigaku BioSAXS-1000","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Calredoxin, Redox protein from Chlamydomonas reinhardtii","short_name":"CRX","sequence":"MCSARSVRVVPRAATFEGLMDDASKAKMEELERRFKMADVDGNGHIDREELRNLLESMESGEVYMMSQHWLPEDELERCMEQYDVNKDGVISFEEFKQIIYDGLLLEGTLAEYESAFKAVDKSGNGTIGATELSKLFASLGNPVSLEKLVDLMQMYDKDDSGQIEFPEFLLMFRNSLLDLKDMTTYMTLDEAGAGSSGSLVDAVEGDMTLIFSEEELDALISANPDKLVVVFGALTWCRPCKGMQRPVQKLAEHYKDHIVFVKLFGNANKQTKRIFKERFQIRSTPCFITLRKGEPVYTQTGSNKEKLEAGLRSLIANPPVGMIYPSAEALAALQDPNSSSVDKLAAALEHHHHHH","organism":"Chlamydomonas reinhardtii","uniprot_code":"A0A2K3DZB3","uniprot_range_first":25,"uniprot_range_last":357,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MIAIRTAHMLSGAQRAGSGTRVPRCSARSVRVVPRAATFEGLMDDASKAKMEELERRFKM\nADVDGNGHIDREELRNLLESMESGEVYMMSQHWLPEDELERCMEQYDVNKDGVISFEEFK\nQIIYDGLLLEGTLAEYESAFKAVDKSGNGTIGATELSKLFASLGNPVSLEKLVDLMQMYD\nKDDSGQIEFPEFLLMFRNSLLDLKDMTTYMTLDEAGAGSSGSLVDAVEGDMTLIFSEEEL\nDALISANPDKLVVVFGALTWCRPCKGMQRPVQKLAEHYKDHIVFVKLFGNANKQTKRIFK\nERFQIRSTPCFITLRKGEPVYTQTGSNKEKLEAGLRSLIANPPVGMIYPSAEALAAL","mw":39.901,"total_mw":39.901,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris, 150 mM NaCl, 1 mM DTT, 5 mM CaCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Calredoxin with Calcium","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Thailand","full_name":"Synchrotron Light Research Institute","webpage":"https://www.slri.or.th"}],"contributor_name":"Ratana","contributor_surname":"Charoenwattanasatien","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2015-11-17","storage_temperature":null,"cell_temperature":20.0,"exposure_time":120.0,"number_of_frames":2,"wavelength":0.15418,"sample_detector_distance":null,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.033,"s_max":6.681,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of Calredoxin with Calcium in 20 mM Tris, 150 mM NaCl, 1 mM DTT, 5 mM CaCl2, pH 8 were collected on a Rigaku BioSAXS-1000 instrument at the Structural Biology Laboratory, Graduate School of Medical Life Science, Yokohama City University (Yokohama, Japan) using a Pilatus 100K detector at a wavelength of λ = 0.15418 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":42.05,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":42.0,"porod_mw_error":null,"pddf_i0":0.7076,"pddf_i0_error":null,"guinier_i0":0.7,"guinier_i0_error":0.003,"pddf_rg":3.19,"pddf_rg_error":null,"guinier_rg":3.06,"guinier_rg_error":0.08,"pddf_dmax":11.56,"pddf_dmax_error":null,"porod_volume":68.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":16,"guinier_point_last":58,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEA9_datloglog_img.png","symmetry":null,"last_modified":"2019-12-17T19:23:05.878669+01:00","bragg_peak":[]}