{"code":"SASDEC3","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.","publication":{"title":"Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.","author_list":"Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA","journal":"Proc Natl Acad Sci U S A","doi":"10.1073/pnas.1704692114","pmid":"28716919","published_date":"2017 Aug 1"},"status":"released","submitted_date":"2018-08-16","released_date":"2018-08-18"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEC3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEC3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEC3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEC3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEC3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEC3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEC3.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Nucleoporin NSP1","short_name":null,"sequence":"> U=p-acetylphenylalanine. \r\nGCNFNTPQQNKTPFSFGTANNNSNTTNQNSSTGAGAFGTGQSTFGFNNSAPNNTNNANSSITPAFGSNNTGNTAFGNSNPTSNVFGSNNSTTNTFGSNSAGTSLFGSSSAQQTKSNGTAGGNTFGSSSLFNNSTNSNTTKPAFGGLNFGGGNNTTPSSTGNANTSNNLFGATANANUA","organism":"Saccharomyces cerevisiae","uniprot_code":"P14907","uniprot_range_first":2,"uniprot_range_last":175,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MNFNTPQQNKTPFSFGTANNNSNTTNQNSSTGAGAFGTGQSTFGFNNSAPNNTNNANSSI\nTPAFGSNNTGNTAFGNSNPTSNVFGSNNSTTNTFGSNSAGTSLFGSSSAQQTKSNGTAGG\nNTFGSSSLFNNSTNSNTTKPAFGGLNFGGGNNTTPSSTGNANTSNNLFGATANANKPAFS\nFGATTNDDKKTEPDKPAFSFNSSVGNKTDAQAPTTGFSFGSQLGGNKTVNEAAKPSLSFG\nSGSAGANPAGASQPEPTTNEPAKPALSFGTATSDNKTTNTTPSFSFGAKSDENKAGATSK\nPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAF\nSFGAKSDEKKDGDASKPAFSFGAKPDENKASATSKPAFSFGAKPEEKKDDNSSKPAFSFG\nAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKS\nDEKKDSDSSKPAFSFGTKSNEKKDSGSSKPAFSFGAKPDEKKNDEVSKPAFSFGAKANEK\nKESDESKSAFSFGSKPTGKEEGDGAKAAISFGAKPEEQKSSDTSKPAFTFGAQKDNEKKT\nEESSTGKSTADVKSSDSLKLNSKPVELKPVSLDNKTLDDLVTKWTNQLTESASHFEQYTK\nKINSWDQVLVKGGEQISQLYSDAVMAEHSQNKIDQSLQYIERQQDELENFLDNFETKTEA\nLLSDVVSTSSGAAANNNDQKRQQAYKTAQTLDENLNSLSSNLSSLIVEINNVSNTFNKTT\nNIDINNEDENIQLIKILNSHFDALRSLDDNSTSLEKQINSIKK","mw":17.635,"total_mw":17.635,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"PBS, 10 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Unlabeled nucleoporin NSP1 (NSP) without denaturant","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Prague, Czech Republic","full_name":"Institute of Biotechnology CAS v.v.i.","webpage":"http://www.ibt.cas.cz/"}],"contributor_name":"Gustavo","contributor_surname":"Fuertes Vives","orcid":"https://orcid.org/0000-0002-8564-8644"}],"concentration_method":null,"concentration_unit":null,"date":"2015-06-24","storage_temperature":null,"cell_temperature":23.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.1,"sample_detector_distance":3.0,"concentration_min":2.0,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.027,"s_max":4.526,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The protein contains a penultimate non-canonical amino acid p-acetylphenylalanine (207 Da) that is represented as U (selenocysteine, 168 Da) in the amino acid sequence for the entry. Therefore, the calculated MW from sequence (MW(expected)) must be adjusted accordingly (ca. 40 Da).","experiment_description":null,"tags":["idp"],"intensity_unit":"arbitrary","experimental_mw":9.5,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":8641.0,"pddf_i0_error":87.1,"guinier_i0":8825.0,"guinier_i0_error":87.1,"pddf_rg":4.108,"pddf_rg_error":0.06,"guinier_rg":4.1,"guinier_rg_error":0.3,"pddf_dmax":15.0,"pddf_dmax_error":null,"porod_volume":45.45,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":13,"guinier_point_last":111,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEC3_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}