{"code":"SASDEC8","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor.","publication":{"title":"Structural Insights into Bacteriophage GIL01 gp7 Inhibition of Host LexA Repressor.","author_list":"Caveney NA, Pavlin A, Caballero G, Bahun M, Hodnik V, de Castro L, Fornelos N, Butala M, Strynadka NCJ","journal":"Structure","doi":"10.1016/j.str.2019.03.019","pmid":"31056420","published_date":"2019 Jul 2"},"status":"released","submitted_date":"2018-11-23","released_date":"2019-05-06"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEC8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEC8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEC8_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEC8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEC8.sascif","experiment":{"instrument":{"detector":{"type":"Hybrid Photon Counting (HPC) X-ray detector","name":"Rigaku HyPix-3000","resolution":100.0},"name":"University of British Columbia","city":"Vancouver","country":"Canada","beamline_name":"Rigaku BioSAXS-2000","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Bacillus thuringiensis LexA repressor","short_name":"LexA","sequence":"MLENMEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYI\r\nRRDPTKPRAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVEEHFPLPASIVSGA\r\nDQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEATVKRFYKEKDHFR\r\nLQPENSSLEPIILKQVSVIGKVIGVYRDLH","organism":"Bacillus thuringiensis","uniprot_code":"A0A2B4EEI3","uniprot_range_first":1,"uniprot_range_last":210,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MLENMEKLTKRQQDILDFIKLKVQEKGYPPSVREIGQAVGLASSSTVHGHLSRLEEKGYI\nRRDPTKPRAIEILGEDRMDTETQSVIQVPIVGKVTAGLPITAVESVEEHFPLPASIVSGA\nDQVFMLRISGDSMIEAGIFDGDLVVVRQQQSAYNGEIVVALTEDNEATVKRFYKEKDHFR\nLQPENSSLEPIILKQVSVIGKVIGVYRDLH","mw":23.394,"total_mw":46.788,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Bacteriophage pGIL01 gp7","short_name":"gp7","sequence":"MRDKLLDFIIELSQSSKQVVSKSYVIDRLMQVTKEDYKELEKNVEGKKDD","organism":"Bacteriophage pGIL01","uniprot_code":"Q7WSG2","uniprot_range_first":1,"uniprot_range_last":50,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MRDKLLDFIIELSQSSKQVVSKSYVIDRLMQVTKEDYKELEKNVEGKKDD","mw":5.921,"total_mw":23.684,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Hepes, 300 mM NaCl, 10% glycerol,","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Bacillus thuringiensis LexA repressor bound to Bacteriophage pGIL01 gp7 (Bt_LexA_GIL01_gp7)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Vancouver, Canada","full_name":"University of British Columbia","webpage":"https://www.ubc.ca/"}],"contributor_name":"Nathanael","contributor_surname":"Caveney","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2017-08-25","storage_temperature":6.0,"cell_temperature":6.0,"exposure_time":300.0,"number_of_frames":12,"wavelength":0.154,"sample_detector_distance":null,"concentration_min":0.75,"concentration_max":12.0,"sample_volume":null,"flow_rate":null,"s_min":0.117,"s_max":6.422,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEC8_fit1_model1_img.png","software":"Chimera","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEC8_fit1_model1.pdb","model_mw":59.42,"bead_radius":null,"log":null,"symmetry":null,"comment":"I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex","user":389}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/LexA_gp7_fit_img.png","software":"CRYSOL","chi_square_value":6.158,"p_value":0.0,"fit_residual_plot":"LexA_gp7_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEC8_fit1.fit","fit_log":null,"software_version":null,"description":"I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex"},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEC8_fit2_model1_img.png","software":"Chimera","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEC8_fit2_model1.pdb","model_mw":59.42,"bead_radius":null,"log":null,"symmetry":null,"comment":"I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, restrained","user":389}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/rc01_LgiSRAn_fit_img.png","software":"SREFLEX","chi_square_value":2.58,"p_value":2e-06,"fit_residual_plot":"rc01_LgiSRAn_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEC8_fit2.fit","fit_log":null,"software_version":null,"description":"I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, restrained"},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEC8_fit3_model1_img.png","software":"Chimera","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEC8_fit3_model1.pdb","model_mw":59.42,"bead_radius":null,"log":null,"symmetry":null,"comment":"I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, unrestrained","user":389}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/uc01_m3mT2gz_fit_img.png","software":"SREFLEX","chi_square_value":1.899,"p_value":4e-05,"fit_residual_plot":"uc01_m3mT2gz_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEC8_fit3.fit","fit_log":null,"software_version":null,"description":"I-TASSER, ClusPro, ModLoop model of LexA-gp7 complex in SREFLEX, unrestrained"}],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of Bacillus thuringiensis LexA repressor bound to Bacteriophage pGIL01 gp7 (Bt_LexA_GIL01_gp7) in 20 mM HEPES, 300 mM NaCl, 10% glycerol, pH 8 were collected on a Rigaku BioSAXS-2000 instrument at the University of British Columbia (Vancouver, Canada) using a Hybrid Pixel Array Detector Rigaku HyOix-3000 detector at a wavelength of λ = 0.154 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 12 mg/ml were measured at 6°C. 12 successive 300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":null,"experimental_mw":108.2,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":1.53,"guinier_i0_error":null,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":4.391,"guinier_rg_error":null,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":null,"guinier_point_last":null,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEC8_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}