{"code":"SASDED2","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Solution model of the intrinsically disordered polyglutamine tract-binding protein-1.","publication":{"title":"Solution model of the intrinsically disordered polyglutamine tract-binding protein-1.","author_list":"Rees M, Gorba C, de Chiara C, Bui TT, Garcia-Maya M, Drake AF, Okazawa H, Pastore A, Svergun D, Chen YW","journal":"Biophys J","doi":"10.1016/j.bpj.2012.02.047","pmid":"22500761","published_date":"2012 Apr 4"},"status":"released","submitted_date":"2018-08-03","released_date":"2018-08-07"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDED2.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDED2.csv","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDED2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDED2_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDED2_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDED2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDED2.sascif","experiment":{"instrument":{"detector":{"type":"Custom-built","name":"Pilatus 1M-W","resolution":0.172},"name":"DORIS III, DESY","city":"Hamburg","country":"Germany","beamline_name":"EMBL X33","beam_geometry":"0.60","type_of_source":"X-ray 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(PQBP-1)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"London, UK","full_name":"King's College London","webpage":"https://www.kcl.ac.uk"}],"contributor_name":"Yu Wai","contributor_surname":"Chen","orcid":"http://orcid.org/0000-0001-7833-7533"}],"concentration_method":null,"concentration_unit":null,"date":"2009-11-18","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":15.0,"number_of_frames":8,"wavelength":0.15,"sample_detector_distance":2.7,"concentration_min":7.0,"concentration_max":13.0,"sample_volume":null,"flow_rate":null,"s_min":0.077,"s_max":6.208,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDED2_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":0.857,"p_value":0.0,"fit_residual_plot":"fit4columns1607_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDED2_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/GA001_LZEuVwU.log","software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDED2_fit2_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"1.1.0 r980","model_data":"https://www.sasbdb.org/media/pdb_file/SASDED2_fit2_model1.pdb","model_mw":40.3,"bead_radius":2.7,"log":null,"symmetry":null,"comment":null,"user":342}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDED2_fit2_fixed_fit_img.png","software":"DAMMIF","chi_square_value":1.018081,"p_value":0.058031,"fit_residual_plot":"SASDED2_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDED2_fit2.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/SVD_1_4.log","software_version":"1.1.0","description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.5a","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of polyglutamine tract-binding protein 1 (PQBP-1) in 20 mM Tris, 150 mM NaCl, 1mM DTT, pH 7 were collected on the EMBL X33 beam line at DORIS III (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 7 and 13 mg/ml were measured at 20°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":["idp","X33"],"intensity_unit":"arbitrary","experimental_mw":30.6,"experimental_mw_error":null,"guinier_i0_mw":30.0,"guinier_i0_mw_error":3.0,"porod_mw":35.0,"porod_mw_error":null,"pddf_i0":40.75,"pddf_i0_error":null,"guinier_i0":40.49,"guinier_i0_error":null,"pddf_rg":3.82,"pddf_rg_error":null,"guinier_rg":3.72,"guinier_rg_error":0.2,"pddf_dmax":13.0,"pddf_dmax_error":null,"porod_volume":51.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":30,"guinier_point_last":97,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDED2_datloglog_img.png","symmetry":null,"last_modified":"2023-05-25T11:21:52.654103+02:00","bragg_peak":[]}