{"code":"SASDED7","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain.","publication":{"title":"Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain.","author_list":"Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA","journal":"Structure","doi":"10.1016/j.str.2019.04.016","pmid":"31178222","published_date":"2019 May 14"},"status":"released","submitted_date":"2018-10-30","released_date":"2019-06-11"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDED7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDED7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDED7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDED7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDED7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDED7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDED7.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Transient receptor potential channel mucolipin 2","short_name":"TRPML2","sequence":"GLSNQLVVAFKEDNTVAFKHLFLKGYSGTDEDDYSCS\r\nVYTQEDAYESIFFAINQYHQLKDITLGTLGYGENEDNRIGLKVCKQHYKKGTMFPSNETL\r\nNIDNDVELDCVQLDLQDLSKKPPDWKNSSFFRLEFYRLLQVEISFHLKGIDLQTIHSREL\r\nPDCYVFQNTIIFDNKAHSGKIKIYFDSDAKIEECKDLNIFGSTQK","organism":"Homo sapiens","uniprot_code":"Q8IZK6","uniprot_range_first":1,"uniprot_range_last":285,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MARQPYRFPQARIPERGSGVFRLTVRNAMAHRDSEMKEECLREDLKFYFMSPCEKYRARR\nQIPWKLGLQILKIVMVTTQLVRFGLSNQLVVAFKEDNTVAFKHLFLKGYSGTDEDDYSCS\nVYTQEDAYESIFFAINQYHQLKDITLGTLGYGENEDNRIGLKVCKQHYKKGTMFPSNETL\nNIDNDVELDCVQLDLQDLSKKPPDWKNSSFFRLEFYRLLQVEISFHLKGIDLQTIHSREL\nPDCYVFQNTIIFDNKAHSGKIKIYFDSDAKIEECKDLNIFGSTQKNAQYVLVFDAFVIVI\nCLASLILCTRSIVLALRLRKRFLNFFLEKYKRPVCDTDQWEFINGWYVLVIISDLMTIIG\nSILKMEIKAKNLTNYDLCSIFLGTSTLLVWVGVIRYLGYFQAYNVLILTMQASLPKVLRF\nCACAGMIYLGYTFCGWIVLGPYHDKFENLNTVAECLFSLVNGDDMFATFAQIQQKSILVW\nLFSRLYLYSFISLFIYMILSLFIALITDSYDTIKKFQQNGFPETDLQEFLKECSSKEEYQ\nKESSAFLSCICCRRRKRSDDHLIPIS","mw":23.354,"total_mw":93.416,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"10 mM HEPES pH 7.4, 150 mM NaCl, 2 mM CaCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 7.4, 2mM Calcium","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL","address":"Grenoble, France","full_name":"European Molecular Biology Laboratory (EMBL) - Grenoble Outstation","webpage":"https://www.embl.fr/"}],"contributor_name":"Martha","contributor_surname":"Brennich","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2018-10-20","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":1200,"wavelength":0.099,"sample_detector_distance":2.849,"concentration_min":0.0,"concentration_max":0.55,"sample_volume":null,"flow_rate":null,"s_min":0.035,"s_max":4.946,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDED7_fit1_model1_img.png","software":"CORAL","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDED7_fit1_model1.pdb","model_mw":92.32,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":119}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/pH7520CaCRP_P1start295_20-1_fit_img.png","software":"CORAL","chi_square_value":1.08,"p_value":0.003595,"fit_residual_plot":"pH7520CaCRP_P1start295_20-1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDED7_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/pH7520CaCRP_P1start295_20.log","software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDED7_fit2_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"r10552","model_data":"https://www.sasbdb.org/media/pdb_file/SASDED7_fit2_model1.pdb","model_mw":92.8,"bead_radius":1.8,"log":null,"symmetry":null,"comment":null,"user":119}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/pH7520Ca89_P1_OBLATE_02_fit_img.png","software":"DAMMIF","chi_square_value":1.106,"p_value":0.607589,"fit_residual_plot":"pH7520Ca89_P1_OBLATE_02_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDED7_fit2.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/pH7520Ca89_P1_OBLATE_02.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The experimental mass was determined via correlated volume.","experiment_description":"Synchrotron SAXS data from solutions of Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain in 10 mM HEPES pH 7.4, 150 mM NaCl, 2 mM CaCl2, were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20°C using the following parameters: Column: Agilent BioSEC 3 bead size 300 μm, Ø 4.6 mm, length 300 mm; Flow rate: 0.3 mL/min; Total acquisition time: 20 min; Injection concentration: 10 mg/mL; Injection volume: 20 μL. The data obtained through the sample elution peak  were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":96.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":84.0,"porod_mw_error":null,"pddf_i0":46.25,"pddf_i0_error":null,"guinier_i0":46.48,"guinier_i0_error":0.12,"pddf_rg":3.362,"pddf_rg_error":null,"guinier_rg":3.39,"guinier_rg_error":0.4,"pddf_dmax":8.9,"pddf_dmax_error":null,"porod_volume":134.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":13,"guinier_point_last":74,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDED7_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}