{"code":"SASDED8","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"β<sub>2</sub>-Type Amyloidlike Fibrils of Poly-l-glutamic Acid Convert into Long, Highly Ordered Helices upon Dissolution in Dimethyl Sulfoxide.","publication":{"title":"β<sub>2</sub>-Type Amyloidlike Fibrils of Poly-l-glutamic Acid Convert into Long, Highly Ordered Helices upon Dissolution in Dimethyl Sulfoxide.","author_list":"Berbeć S, Dec R, Molodenskiy D, Wielgus-Kutrowska B, Johannessen C, Hernik-Magoń A, Tobias F, Bzowska A, Ścibisz G, Keiderling TA, Svergun D, Dzwolak W","journal":"J Phys Chem B","doi":"10.1021/acs.jpcb.8b08308","pmid":"30427196","published_date":"2018 Dec 20"},"status":"released","submitted_date":"2018-11-25","released_date":"2018-11-27"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDED8.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDED8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDED8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDED8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDED8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDED8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDED8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Poly-L-Glutamic Acid","short_name":"PLGA","sequence":"HO2CCH2CH2CH(NH2)CO2H","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"other","molecular_type":"other","uniprot_sequence":null,"mw":0.147,"total_mw":6.174,"number_molecules":42,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"Dimethyl Sulfoxide (DMSO)","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":null,"deuteration":null},"purity_method":null,"name":"Poly-L-Glutamic Acid in Dimethyl Sulfoxide (DMSO)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Dima","contributor_surname":"Molodenskiy","orcid":"https://orcid.org/0000-0002-5954-4294"}],"concentration_method":null,"concentration_unit":null,"date":"2017-12-11","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.045,"number_of_frames":40,"wavelength":0.124,"sample_detector_distance":null,"concentration_min":10.0,"concentration_max":30.0,"sample_volume":null,"flow_rate":null,"s_min":0.093,"s_max":5.061,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDED8_fit1_model1_img.png","software":"PyMOL","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDED8_fit1_model1.pdb","model_mw":null,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/42a00.log","symmetry":null,"comment":"The model was built by a custom python script","user":372}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/42a00_fit_img.png","software":"CRYSOL","chi_square_value":0.637,"p_value":0.194877,"fit_residual_plot":"42a00_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDED8_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from Poly-L-Glutamic Acid (PGLA) in neat dimethyl sulfoxide were collected on the EMBL-P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 10 and 30 mg/ml were measured at 20°C. 40 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. 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