{"code":"SASDEE8","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Farnesylation of human guanylate binding protein 1 as safety mechanism preventing structural rearrangements and uninduced dimerization.","publication":{"title":"Farnesylation of human guanylate binding protein 1 as safety mechanism preventing structural rearrangements and uninduced dimerization.","author_list":"Lorenz C, Ince S, Zhang T, Cousin A, Batra-Safferling R, Nagel-Steger L, Herrmann C, Stadler AM","journal":"FEBS J","doi":"10.1111/febs.15015","pmid":"31330084","published_date":"2019 Jul 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Jülich","webpage":"https://www.fz-juelich.de"}],"contributor_name":"Charlotte","contributor_surname":"Lorenz","orcid":"https://orcid.org/0000-0003-3614-341X"}],"concentration_method":null,"concentration_unit":null,"date":"2017-04-30","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":3600,"wavelength":0.09919,"sample_detector_distance":2.867,"concentration_min":null,"concentration_max":12.7,"sample_volume":null,"flow_rate":null,"s_min":0.033,"s_max":4.944,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEE8_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.098,"p_value":0.002911,"fit_residual_plot":"SASDEE8_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEE8_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_farn.log","software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEE8_fit2_model1_img.png","software":"PyMOL","pdb_link":[{"pdb_code":"1dg3","ext_code":"1DG3","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEE8_fit2_model1.pdb","model_mw":62.07,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":354}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEE8_fit2_fixed_fit_img.png","software":"CRYSOL","chi_square_value":2.61,"p_value":0.0,"fit_residual_plot":"SASDEE8_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEE8_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/1DG300_chl3COM.log","software_version":null,"description":null},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEE8_fit3_model1_img.png","software":"SASREF","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEE8_fit3_model1.pdb","model_mw":65.32,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/farn_NF_1.log","symmetry":null,"comment":null,"user":354}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEE8_fit3_fixed_fit_img.png","software":"SASREF","chi_square_value":3.02,"p_value":0.0,"fit_residual_plot":"SASDEE8_fit3_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEE8_fit3.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/farn_NF_1.log","software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Farnesylated human Guanylate Binding Protein 1 (farn-hGBP1) in 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, pH 7.9 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20°C using the following parameters: Column: UNKNOWN; Flow rate: UNKNOWN mL/min; Total acquisition time: 3600 s; Sample injection concentration: 12.70 mg/mL; Injection volume: UNKNOWN μL. The data obtained through the sample elution peak (collected as successive 1 s frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":63.82,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":63.82,"porod_mw_error":null,"pddf_i0":43.82,"pddf_i0_error":null,"guinier_i0":43.69,"guinier_i0_error":0.033,"pddf_rg":3.965,"pddf_rg_error":null,"guinier_rg":3.85,"guinier_rg_error":0.05,"pddf_dmax":12.97,"pddf_dmax_error":null,"porod_volume":102.11,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":13,"guinier_point_last":53,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEE8_datloglog_img.png","symmetry":null,"last_modified":"2020-08-28T15:28:10.997868+02:00","bragg_peak":[]}