{"code":"SASDEH8","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Farnesylation of human guanylate binding protein 1 as safety mechanism preventing structural rearrangements and uninduced dimerization.","publication":{"title":"Farnesylation of human guanylate binding protein 1 as safety mechanism preventing structural rearrangements and uninduced dimerization.","author_list":"Lorenz C, Ince S, Zhang T, Cousin A, Batra-Safferling R, Nagel-Steger L, Herrmann C, Stadler AM","journal":"FEBS J","doi":"10.1111/febs.15015","pmid":"31330084","published_date":"2019 Jul 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GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Human Guanylate Binding Protein 1 with guanylyl imidodiphosphate (hGBP1 + GppNHp) in 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, 0.2 mM GppNHp pH 7.9 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20°C using the following parameters: Column: UNKNOWN; Flow rate: UNKNOWN mL/min; Total acquisition time: 3600 s; Sample injection concentration: 55 mg/mL; Injection volume: UNKNOWN μL. The data obtained through the sample elution peak (collected as successive 1 s frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":74.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":74.78,"porod_mw_error":null,"pddf_i0":241.4,"pddf_i0_error":null,"guinier_i0":237.7,"guinier_i0_error":0.18,"pddf_rg":4.837,"pddf_rg_error":null,"guinier_rg":4.49,"guinier_rg_error":0.04,"pddf_dmax":20.5,"pddf_dmax_error":null,"porod_volume":119.64,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":18,"guinier_point_last":37,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEH8_datloglog_img.png","symmetry":null,"last_modified":"2020-08-28T15:28:12.277933+02:00","bragg_peak":[]}