{"code":"SASDEJ7","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes.","publication":{"title":"Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes.","author_list":"Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M","journal":"J Mol Biol","doi":"10.1016/j.jmb.2019.07.004","pmid":"31288030","published_date":"2019 Sep 6"},"status":"released","submitted_date":"2018-11-02","released_date":"2019-09-13"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEJ7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEJ7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEJ7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEJ7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEJ7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEJ7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEJ7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Surface presentation of antigens protein SpaO SpaO(SPOA2)","short_name":"SpaOc","sequence":"METLDIQHIEEENNTTET AETLPGLNQLPVKLEFVLYRKNVTLAELEA \r\nMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESGNGESAWSHPQFEK","organism":"Salmonella enterica subsp. enterica serovar Typhimurium","uniprot_code":"P40699","uniprot_range_first":203,"uniprot_range_last":303,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MSLRVRQIDRREWLLAQTATECQRHGREATLEYPTRQGMWVRLSDAEKRWSAWIKPGDWL\nEHVSPALAGAAVSAGAEHLVVPWLAATERPFELPVPHLSCRRLCVENPVPGSALPEGKLL\nHIMSDRGGLWFEHLPELPAVGGGRPKMLRWPLRFVIGSSDTQRSLLGRIGIGDVLLIRTS\nRAEVYCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYR\nKNVTLAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESG\nNGE","mw":12.374,"total_mw":24.748,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Surface presentation of antigens protein SpaO(SPOA1,2)","short_name":"SpaO","sequence":"MSLRVRQIDRREWLLAQTATECQRHGREATLEYPTRQGMWVRLSDAEKRW \r\nSAWIKPGDWLEHVSPALAGAAVSAGAEHLVVPWLAATERPFELPVPHLSC \r\nRRLCVENPVPGSALPEGKLLHIMSDRGGLWFEHLPELPAVGGGRPKMLRW \r\nPLRFVIGSSDTQRSLLGRIGIGDVLLIRTSRAEVYCYAKKLGHFNRVEGG \r\nIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYRKNVTLAELEA \r\nMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESG \r\nNGE","organism":"Salmonella enterica subsp. enterica serovar Typhimurium","uniprot_code":"P40699","uniprot_range_first":1,"uniprot_range_last":303,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSLRVRQIDRREWLLAQTATECQRHGREATLEYPTRQGMWVRLSDAEKRWSAWIKPGDWL\nEHVSPALAGAAVSAGAEHLVVPWLAATERPFELPVPHLSCRRLCVENPVPGSALPEGKLL\nHIMSDRGGLWFEHLPELPAVGGGRPKMLRWPLRFVIGSSDTQRSLLGRIGIGDVLLIRTS\nRAEVYCYAKKLGHFNRVEGGIIVETLDIQHIEEENNTTETAETLPGLNQLPVKLEFVLYR\nKNVTLAELEAMGQQQLLSLPTNAELNVEIMANGVLLGNGELVQMNDTLGVEIHEWLSESG\nNGE","mw":33.793,"total_mw":33.793,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Oxygen-regulated invasion protein 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InvC","short_name":"InvC","sequence":"MKTPRLLQYLAYPQKITGPIIEAELRDVAIGELCEIRRGWHQKQVVARAQVVGLQRERTV\r\nLSLIGNAQGLSRDVVLYPTGRALSAWVGYSVLGAVLDPTGKIVERFTPEVAPISEERVID\r\nVAPPSYASRVGVREPLITGVRAIDGLLTCGVGQRMGIFASAGCGKTMLMHMLIEQTEADV\r\nFVIGLIGERGREVTEFVDMLRASHKKEKCVLVFATSDFPSVDRCNAAQLATTVAEYFRDQ\r\nGKRVVLFIDSMTRYARALRDVALASGERPARRGYPASVFDNLPRLLERPGATSEGSITAF\r\nYTVLLESEEEADPMADEIRSILDGHLYLSRKLAGQGHYPAIDVLKSVSRVFGQVTTPTHA\r\nEQASAVRKLMTRLEELQLFIDLGEYRPGENIDNDRAMQMRDSLKAWLCQPVAQYSSFDDT\r\nLSGMNAFADQN","organism":"Salmonella enterica subsp. enterica serovar Typhimurium","uniprot_code":"E8XL22","uniprot_range_first":1,"uniprot_range_last":431,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MKTPRLLQYLAYPQKITGPIIEAELRDVAIGELCEIRRGWHQKQVVARAQVVGLQRERTV\nLSLIGNAQGLSRDVVLYPTGRALSAWVGYSVLGAVLDPTGKIVERFTPEVAPISEERVID\nVAPPSYASRVGVREPLITGVRAIDGLLTCGVGQRMGIFASAGCGKTMLMHMLIEQTEADV\nFVIGLIGERGREVTEFVDMLRASHKKEKCVLVFATSDFPSVDRCNAAQLATTVAEYFRDQ\nGKRVVLFIDSMTRYARALRDVALASGERPARRGYPASVFDNLPRLLERPGATSEGSITAF\nYTVLLESEEEADPMADEIRSILDGHLYLSRKLAGQGHYPAIDVLKSVSRVFGQVTTPTHA\nEQASAVRKLMTRLEELQLFIDLGEYRPGENIDNDRAMQMRDSLKAWLCQPVAQYSSFDDT\nLSGMNAFADQN","mw":47.61,"total_mw":47.61,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"10 mM Tris-HCl, 50 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"The InvC-ATP synthase, surface presentation of antigens and oxygen-regulated invasion protein (ISO) complex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Anne","contributor_surname":"Tuukkanen","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-04-12","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":3600,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":1.0,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.118,"s_max":2.856,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"SEC-SAXS was performed at 20°C using the following parameters: Column type, XXXX; Flow rate: XXXX mL/min; Total acquisition time: 60 min (3600 x 1 s frames); Injection concentration: XXXX mg/mL; Injection volume: XXXX μL.","experiment_description":"Synchrotron SAXS data from solutions of ISO complex in 10 mM Tris-HCl, 50 mM NaCl, pH 8.0 were collected on the EMBL P12 beam line using size exclusion chromatography SAXS (SEC-SAXS) at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data obtained through the sample SEC-SAXS elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":185.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":188.0,"porod_mw_error":null,"pddf_i0":4593.0,"pddf_i0_error":null,"guinier_i0":4176.6,"guinier_i0_error":77.0,"pddf_rg":6.957,"pddf_rg_error":0.7,"guinier_rg":6.03,"guinier_rg_error":0.13,"pddf_dmax":22.7,"pddf_dmax_error":2.3,"porod_volume":301.93,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":40,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEJ7_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}