{"code":"SASDEL4","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"The dynamic nature of the conserved tegument protein UL37 of herpesviruses.","publication":{"title":"The dynamic nature of the conserved tegument protein UL37 of herpesviruses.","author_list":"Koenigsberg AL, Heldwein EE","journal":"J Biol Chem","doi":"10.1074/jbc.RA118.004481","pmid":"30166339","published_date":"2018 Oct 12"},"status":"released","submitted_date":"2018-07-16","released_date":"2018-09-07"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEL4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEL4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEL4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEL4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEL4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEL4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEL4.sascif","experiment":{"instrument":{"detector":{"type":"Pilatus","name":"Pilatus 200K","resolution":172.0},"name":"Cornell High Energy Synchrotron Source (CHESS)","city":"Ithaca, NY","country":"USA","beamline_name":"G1","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Tegument protein UL37","short_name":"PRV UL37C","sequence":"GPGSGLRADGAGETPMEAPTSAALVDLAAAADLVNYPSMVHLEAMADPSFAPFFVVTAVADAYNCLLTASYDRRADLAQVLTWARDYGAGLIPNVSGYRTKLAALVAALAREDLTVAHARNVEALAHELHGVVRAAAEVLEAPAPAPPSLAASAFLGDLLARALRRRLEDMATHTGDLLDSMATAAGAVAERVVALERLFTCRFVFRGRTASVFAPGEGGPCLGTWRLADVVDAVGAFRAEVNTHRSDMRADAGALRGVMAQTTEALRECEALGLQAPVFRTLCADHARLSKLQTSAALTAGRLQAATVLRPVERFLARWDLVSAALRRAVDERGVLALVATLRETWTEAREEQAPPAPAFTPEELREAADRVLGDYHEVAGDGEAGPDAVPLSASVNVRDWAAVNLEVLR","organism":"Suid alphaherpesvirus 1","uniprot_code":"Q911W0","uniprot_range_first":478,"uniprot_range_last":884,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MEALVRALEEADHAVATVVQSRILEFFMAAGRETPAGVRGLWARALRLACRAYVETGTCE\nAAVLAENLAGLALWRLRHDWDEGTAPLLELLGVVNGDDTTAALTEAGLRTSAEFGPDAMF\nRLVSEWCAAFDEALAGARSADDVLAAPRVVPPEQTARALVQPRFATLYDMDFVQDGLRYV\nAQHTNWALPLALAVRQMQNEGLKPLTRALFALTIADEFFHDRQNPTLREQFAEAARAVDE\nAALVPVGEVNATPRTAVEVRVSAALAHGDAYVRELRPGTVARRLRTDQGVLALLDPGAQA\nVHVAAAADLDHTQVDATGVWEAVQASASPLQVVEALVTAGFTRRHCDLLERAVLDRAPRL\nTDAQRAVGCTAVVGGVVHRLLDDYGPGLDYVRAYTDVADTLEPLYGDVTAALGLPEKGVE\nHVVRHCMAPRPPTEHVGAARAALLREVAAAERRAGLAHSAAREALNTWLAFRAQSRWGLR\nADGAGETPMEAPTSAALVDLAAAADLVNYPSMVHLEAMADPSFAPFFVVTAVADAYNCLL\nTASYDRRADLAQVLTWARDYGAGLIPNVSGYRTKLAALVAALAREDLTVAHARNVEALAH\nELHGVVRAAAEVLEAPAPAPPSLAASAFLGDLLARALRRRLEDMATHTGDLLDSMATAAG\nAVAERVVALERLFTCRFVFRGRTASVFAPGEGGPCLGTWRLADVVDAVGAFRAEVNTHRS\nDMRADAGALRGVMAQTTEALRECEALGLQAPVFRTLCADHARLSKLQTSAALTAGRLQAA\nTVLRPVERFLARWDLVSAALRRAVDERGVLALVATLRETWTEAREEQAPPAPAFTPEELR\nEAADRVLGDYHEVAGDGEAGPDAVPLSASVNVRDWAAVNLEVLRRGARAPDDASAGVPLL\nAQTWVPVDRLLAEVDAVSK","mw":43.456,"total_mw":43.456,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"100 mM HEPES 150 mM NaCl 5% glycerol 0.1 mM tris(2-carboxyethyl)phosphine (TCEP)","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Residues 478-884 of pseudorabies virus tegument protein UL37","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Somerville, MA, USA","full_name":"Tufts University School of Medicine","webpage":"https://www.tufts.edu/"}],"contributor_name":"Andrea","contributor_surname":"Koenigsberg","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2017-06-03","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":2.0,"number_of_frames":300,"wavelength":0.125,"sample_detector_distance":1.5,"concentration_min":6.0,"concentration_max":null,"sample_volume":null,"flow_rate":null,"s_min":0.074,"s_max":2.808,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEL4_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEL4_fit1_model1.pdb","model_mw":51.935,"bead_radius":6.4,"log":"https://www.sasbdb.org/media/log_files/pAK13_25_3.log","symmetry":"None","comment":null,"user":343}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/pAK13_25_3_fit_img.png","software":"DAMMIN","chi_square_value":1.077,"p_value":0.37201,"fit_residual_plot":"pAK13_25_3_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEL4_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/pAK13_25_3.log","software_version":"5.3","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data, I(s) vs s (where s = 4πsinθ/λ, and 2θ is the scattering angle), were measured from the C-terminal half of the Pseudorabiesvirus tegument protein UL37 using continuous-flow size-exclusion chromatography SAXS (SEC-SAXS) on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS; Ithaca, NY, USA). Data were collected a using a Pilatus 200K detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.125 nm. The SEC mobile phase consisted of 100 mM HEPES, 150 mM NaCl, 5% glycerol, 0.1 mM tris(2-carboxyethyl)phosphine (TCEP), pH 7.5, (4°C). The SAXS data measured from the SEC-elution (sample peak and buffer; 300 successive 2 s frames) were normalized to the intensity of the transmitted beam and radially averaged. The scattering of an appropriate solvent-blank was subtracted from the sample frames to produce the scaled and averaged data displayed in this entry.","tags":[],"intensity_unit":"1/cm","experimental_mw":44.0,"experimental_mw_error":null,"guinier_i0_mw":44.0,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.008036,"pddf_i0_error":null,"guinier_i0":0.0081,"guinier_i0_error":7e-05,"pddf_rg":3.99,"pddf_rg_error":null,"guinier_rg":3.86,"guinier_rg_error":0.014,"pddf_dmax":12.5,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":null,"guinier_point_last":null,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEL4_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}