{"code":"SASDEM9","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Complementary substrate specificity and distinct quaternary assembly of the <i>Escherichia coli</i> aerobic and anaerobic beta-oxidation trifunctional enzyme complexes.","publication":{"title":"Complementary substrate specificity and distinct quaternary assembly of the <i>Escherichia coli</i> aerobic and anaerobic beta-oxidation trifunctional enzyme complexes.","author_list":"Sah-Teli SK, Hynönen MJ, Schmitz W, Geraets JA, Seitsonen J, Pedersen JS, Butcher SJ, Wierenga RK, Venkatesan R","journal":"Biochem J","doi":"10.1042/BCJ20190314","pmid":"31235482","published_date":"2019 Jun 25"},"status":"released","submitted_date":"2018-12-28","released_date":"2019-07-02"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEM9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEM9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEM9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEM9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEM9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEM9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEM9.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray 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Fatty acid oxidation complex subunit alpha","short_name":"anEcTFE-alpha","sequence":">anEcTFEalpha\r\nMEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFIAGADINMIG\r\nNCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSG\r\nGTQRLPRLIGVSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGR\r\nALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPG\r\nSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLA\r\nLISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP\r\nVEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVK\r\nFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPA\r\nIYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVV\r\nAIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ","organism":"Escherichia 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3-ketoacyl-CoA thiolase FadI beta subunit","short_name":"anEcTFE-beta","sequence":">anEcTFEbeta\r\nMGSSHHHHHHSQDPMGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPN\r\nIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKA\r\nRTMSQRLKLFSRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEE\r\nVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY\r\nLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGE\r\nVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE","organism":"Escherichia 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subunit","short_name":"anEcTFE-beta","sequence":">anEcTFEbeta\r\nMGSSHHHHHHSQDPMGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPN\r\nIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKA\r\nRTMSQRLKLFSRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEE\r\nVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY\r\nLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSDLTLIDMHEAFAAQTLANIQLLGSERFAREALGRAHATGE\r\nVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE","organism":"Escherichia 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DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"anEcTFE: Escherichia coli anaerobic fatty acid beta-oxidation trifunctional enzyme complex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Finland","full_name":"University of Oulu","webpage":"https://www.oulu.fi"}],"contributor_name":"shiv k.","contributor_surname":"sah-teli","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2015-09-22","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":1200,"wavelength":0.1,"sample_detector_distance":2.867,"concentration_min":null,"concentration_max":3.5,"sample_volume":null,"flow_rate":null,"s_min":0.05,"s_max":1.711,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEM9_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEM9_fit1_model1.pdb","model_mw":487.865,"bead_radius":5.0,"log":"https://www.sasbdb.org/media/log_files/anectfed.log","symmetry":"p1","comment":null,"user":400}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/anectfed_fit_img.png","software":"DAMMIN","chi_square_value":1.01,"p_value":0.000235,"fit_residual_plot":"anectfed_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEM9_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/anectfed.log","software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEM9_fit2_model1_img.png","software":"SwissModel","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEM9_fit2_model1.pdb","model_mw":483.1,"bead_radius":null,"log":null,"symmetry":null,"comment":"subunits and complex were modeled using swissmodel and coot respectively  using 5zqz.pdb as referenc","user":400}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/anEcTFE-modelon5zqz-octamer8-5zqz-templates00_fit_img.png","software":"CRYSOL","chi_square_value":3.501,"p_value":0.0,"fit_residual_plot":"anEcTFE-modelon5zqz-octamer8-5zqz-templates00_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEM9_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/anEcTFE-modelon5zqz-octamer8-5zqz-templates00.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of anEcTFE in 50 mM Tris, 500 mM NaCl, 5% glycerol, 0.05% C12E9 (1-O-(n-Dodecyl)-nonaethyleneglycol), 2.5 mM DTT, pH 8 were measured at 20°C using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data were collected as 1200 successive 1 second frames through the SEC elution profile  (sample concentration = 3.5 mg/ml ). The SAXS data were measured from the sample and buffer from a Superdex 200 5/150 GL, (GE Healthcare) column at flow rate of 0.16 mL/min. The sample-peak data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":535.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":535.0,"porod_mw_error":null,"pddf_i0":58.74,"pddf_i0_error":null,"guinier_i0":58.9,"guinier_i0_error":0.14,"pddf_rg":6.15,"pddf_rg_error":null,"guinier_rg":6.19,"guinier_rg_error":0.02,"pddf_dmax":19.57,"pddf_dmax_error":null,"porod_volume":856.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":35,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEM9_datloglog_img.png","symmetry":null,"last_modified":"2021-02-21T15:44:53.898719+01:00","bragg_peak":[]}