{"code":"SASDEP9","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity.","publication":{"title":"cGAS facilitates sensing of extracellular cyclic dinucleotides to activate innate immunity.","author_list":"Liu H, Moura-Alves P, Pei G, Mollenkopf HJ, Hurwitz R, Wu X, Wang F, Liu S, Ma M, Fei Y, Zhu C, Koehler AB, Oberbeck-Mueller D, Hahnke K, Klemm M, Guhlich-Bornhof U, Ge B, Tuukkanen A, Kolbe M, Dorhoi A, Kaufmann SH","journal":"EMBO Rep","doi":"10.15252/embr.201846293","pmid":"30872316","published_date":"2019 Mar 14"},"status":"released","submitted_date":"2019-01-14","released_date":"2019-03-20"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEP9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEP9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEP9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEP9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEP9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEP9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEP9.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Cyclic GMP-AMP synthase","short_name":"cGAS","sequence":"MQPWHGKAMQRASEAGATAPKASARNARGAPMDPTESPAAPEAALPKAGKFGPARKSGSRQKKSAPDTQERPPVRATGARAKKAPQRAQDTQPSDATSAPGAEGLEPPAAREPALSRAGSCRQRGARCSTKPRPPPGPWDVPSPGLPVSAPILVRRDAAPGASKLRAVLEKLKLSRDDISTAAGMVKGVVDHLLLRLKCDSAFRGVGLLNTGSYYEHVKISAPNEFDVMFKLEVPRIQLEEYSNTRAYYFVKFKRNPKENPLSQFLEGEILSASKMLSKFRKIIKEEINDIKDTDVIMKRKRGGSPAVTLLISEKISVDITLALESKSSWPASTQEGLRIQNWLSAKVRKQLRLKPFYLVPKHAKEGNGFQEETWRLSFSHIEKEILNNHGKSKTCCENKEEKCCRKDCLKLMKYLLEQLKERFKDKKHLDKFSSYHVKTAFFHVCTQNPQDSQWDRKDLGLCFDNCVTYFLQCLRTEKLENYFIPEFNLFSSNLIDKRSKEFLTKQIEYERNNEFPVFDEFLEYPYDVPDYAAAALEHHHHHH","organism":"Homo sapiens","uniprot_code":"Q8N884","uniprot_range_first":1,"uniprot_range_last":522,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MQPWHGKAMQRASEAGATAPKASARNARGAPMDPTESPAAPEAALPKAGKFGPARKSGSR\nQKKSAPDTQERPPVRATGARAKKAPQRAQDTQPSDATSAPGAEGLEPPAAREPALSRAGS\nCRQRGARCSTKPRPPPGPWDVPSPGLPVSAPILVRRDAAPGASKLRAVLEKLKLSRDDIS\nTAAGMVKGVVDHLLLRLKCDSAFRGVGLLNTGSYYEHVKISAPNEFDVMFKLEVPRIQLE\nEYSNTRAYYFVKFKRNPKENPLSQFLEGEILSASKMLSKFRKIIKEEINDIKDTDVIMKR\nKRGGSPAVTLLISEKISVDITLALESKSSWPASTQEGLRIQNWLSAKVRKQLRLKPFYLV\nPKHAKEGNGFQEETWRLSFSHIEKEILNNHGKSKTCCENKEEKCCRKDCLKLMKYLLEQL\nKERFKDKKHLDKFSSYHVKTAFFHVCTQNPQDSQWDRKDLGLCFDNCVTYFLQCLRTEKL\nENYFIPEFNLFSSNLIDKRSKEFLTKQIEYERNNEFPVFDEF","mw":61.419,"total_mw":61.419,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM HEPES","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Cyclic GMP-AMP synthase (cGAS)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Anne","contributor_surname":"Tuukkanen","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-04-25","storage_temperature":10.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":3600,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":9.0,"sample_volume":null,"flow_rate":null,"s_min":0.185,"s_max":5.025,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEP9_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEP9_fit1_model1.pdb","model_mw":62.53,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":27},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEP9_fit1_model2_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEP9_fit1_model2.pdb","model_mw":62.53,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":27},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEP9_fit1_model3_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEP9_fit1_model3.pdb","model_mw":62.53,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":27},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEP9_fit1_model4_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEP9_fit1_model4.pdb","model_mw":62.53,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":27},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEP9_fit1_model5_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEP9_fit1_model5.pdb","model_mw":62.53,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":27},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEP9_fit1_model6_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEP9_fit1_model6.pdb","model_mw":62.53,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":27}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEP9_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.211,"p_value":0.006468,"fit_residual_plot":"SASDEP9_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEP9_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of cyclic GMP-AMP synthase (cGAS) in 20 mM HEPES, pH 7.4 were measured using size-exclusion chromatography SAXS (SEC-SAXS) on the EMBL P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20 °C using the following parameters: Column: XXXX ; Flow rate: 0.5 mL/min; Total acquisition time: 3600 x 1 s frames; Sample injection concentration: 9 mg/mL; Injection volume: 75 μL. The data obtained through the sample elution peak were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":64.7,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":64.7,"porod_mw_error":null,"pddf_i0":2237.0,"pddf_i0_error":22.4,"guinier_i0":2166.9,"guinier_i0_error":18.2,"pddf_rg":3.384,"pddf_rg_error":0.2,"guinier_rg":3.14,"guinier_rg_error":1.4,"pddf_dmax":12.69,"pddf_dmax_error":0.6,"porod_volume":110.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":14,"guinier_point_last":84,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEP9_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}