{"code":"SASDET5","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Structural studies of Old Yellow Enzyme of Leishmania braziliensis in solution.","publication":{"title":"Structural studies of Old Yellow Enzyme of Leishmania braziliensis in solution.","author_list":"Walbert Veloso-Silva LL, Dores-Silva PR, Bertolino Reis DE, Moreno Oliveira LF, Libardi SH, Borges JC","journal":"Arch Biochem Biophys","doi":"10.1016/j.abb.2018.11.009","pmid":"30447208","published_date":"2018 Nov 14"},"status":"released","submitted_date":"2018-10-05","released_date":"2018-11-19"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDET5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDET5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDET5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDET5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDET5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDET5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDET5.sascif","experiment":{"instrument":{"detector":{"type":"20Hz","name":"Pilatus 300K","resolution":null},"name":"Brazilian Synchrotron Light Laboratory","city":"Campinas","country":"Brazil","beamline_name":"SAXS1 Beamline","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Old Yellow Enzyme of Leishmania braziliensis","short_name":"LbOYE","sequence":">LbOYE_deltaHis\r\nGSEFDYTMPTASNSIDALLKPLVIGQLSMPNRFVMAPLTRCRADDEHVPTPAMVRHYADR\r\nASMGLIITEATQIEKGYSTFCHEGGIYGKEQVDGWRKVTDAVHEKGGLIFCQIHNGGRAT\r\nVPSNVDEGVRIVAPSAVAITDHECPGAFARNGEKQPYPVPHEMTAEDIALYVKLYATAAC\r\nNAMAAGFDGVEVHGANGYLIDEFLKSSSNQRTDEYGGSIENRCRFLFEVLDAVIKAIGRE\r\nRVGLRISPLNSFNSQSDENPEALTRYICSQLNSRNISFLDVMRGDFFSQACGADKWAREA\r\nYEGVLFTGMGFEIEEAAETVASGAADAVVFGTKALANPDLVARAIAGAALNTPDPATFYS\r\nTSEAGYNDYPSMVSSCPSAAAPSCTKETP","organism":"Leishmania braziliensis","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":null,"mw":41.859,"total_mw":41.859,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris-HCl 100 mM NaCl and 1 mM β-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Old Yellow Enzyme of Leishmania braziliensis","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"São Carlos - SP - Brasil","full_name":"Instituto de Química de São Carlos","webpage":""}],"contributor_name":"Júlio","contributor_surname":"Borges","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-05-26","storage_temperature":4.0,"cell_temperature":25.0,"exposure_time":360.0,"number_of_frames":1,"wavelength":0.148,"sample_detector_distance":1.0,"concentration_min":null,"concentration_max":3.0,"sample_volume":null,"flow_rate":null,"s_min":0.234,"s_max":3.181,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDET5_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDET5_fit1_model1.pdb","model_mw":43.075,"bead_radius":2.4,"log":null,"symmetry":null,"comment":null,"user":161}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/LbOYE_fit_img.png","software":"DAMMIN","chi_square_value":6.159,"p_value":2e-06,"fit_residual_plot":"LbOYE_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDET5_fit1.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of old yellow enzyme in 25 mM Tris-HCl 100 mM NaCl and 1 mM β-mercaptoethanol, pH 8 were collected on the SAXS1 beam line at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) using a 20Hz Pilatus 300K detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.148 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 25°C. One 360 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":40.0,"experimental_mw_error":null,"guinier_i0_mw":48.6,"guinier_i0_mw_error":4.0,"porod_mw":40.0,"porod_mw_error":null,"pddf_i0":0.3768,"pddf_i0_error":0.037,"guinier_i0":0.381,"guinier_i0_error":0.0007,"pddf_rg":2.69,"pddf_rg_error":0.2,"guinier_rg":2.71,"guinier_rg_error":null,"pddf_dmax":9.5,"pddf_dmax_error":1.0,"porod_volume":73.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":16,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDET5_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}