{"code":"SASDET9","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"MglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus.","publication":{"title":"MglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus.","author_list":"Galicia C, Lhospice S, Varela PF, Trapani S, Zhang W, Navaza J, Herrou J, Mignot T, Cherfils J","journal":"Nat Commun","doi":"10.1038/s41467-019-13274-3","pmid":"31757955","published_date":"2019 Nov 22"},"status":"released","submitted_date":"2019-01-17","released_date":"2019-12-11"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDET9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDET9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDET9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDET9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDET9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDET9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDET9.sascif","experiment":{"instrument":{"detector":{"type":"CCD","name":"AVIEX PCCD170170","resolution":null},"name":"SOLEIL","city":"Saint-Aubin","country":"France","beamline_name":"SWING","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Gliding motility protein MglB","short_name":"MglB","sequence":"GMGTQLVMYEEEFTKINAVCDRLTKDANAKVVFLVDKNGQLISSAGQTQNIDTTSLASLTAGNVAAMGGLAKLIGENEFPNQFHEGAKDSLYMTIVGSRVVLVVIFDNRTSLGLVRLRIKKASDELTKIFESLVKKTDSPGAGSPFAEISDDDIDNLFSE","organism":"Myxococcus xanthus","uniprot_code":"Q1DB03","uniprot_range_first":1,"uniprot_range_last":159,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MGTQLVMYEEEFTKINAVCDRLTKDANAKVVFLVDKNGQLISSAGQTQNIDTTSLASLTA\nGNVAAMGGLAKLIGENEFPNQFHEGAKDSLYMTIVGSRVVLVVIFDNRTSLGLVRLRIKK\nASDELTKIFESLVKKTDSPGAGSPFAEISDDDIDNLFSE","mw":17.249,"total_mw":34.498,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"150 mM NaCl, 1 mM DTT, 20 mM Tris-HCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Gliding motility protein MglB: A GAP of Myxococcus xanthus MglA","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Cachan, France","full_name":"CNRS UMR8113, ENS Paris-Saclay","webpage":"http://lbpa.ens-paris-saclay.fr/"}],"contributor_name":"Wenhua","contributor_surname":"Zhang","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-10-09","storage_temperature":15.0,"cell_temperature":15.0,"exposure_time":null,"number_of_frames":null,"wavelength":0.103,"sample_detector_distance":1.8,"concentration_min":null,"concentration_max":15.0,"sample_volume":null,"flow_rate":null,"s_min":0.061,"s_max":5.689,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDET9_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDET9_fit1_model1.pdb","model_mw":null,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/WZ-GS-F1_1.log","symmetry":"P2","comment":null,"user":84}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDET9_fit1_fixed_fit_img.png","software":"GASBOR","chi_square_value":0.68,"p_value":0.0,"fit_residual_plot":"SASDET9_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDET9_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/WZ-GS-F1_1.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"X-ray exposure time = UNKNOWN.","experiment_description":"Synchrotron SAXS data from solutions of the gliding motility protein MglB in 150 mM NaCl, 1 mM DTT, 20 mM Tris-HCl, pH 8 were collected on the SWING beam line at SOLEIL (Saint-Aubin, France) using a CCD AVIEX PCCD170170 detector at a sample-detector distance of 1.8 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 15.00 mg/ml was measured at 15°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":36.5,"experimental_mw_error":null,"guinier_i0_mw":37.1,"guinier_i0_mw_error":null,"porod_mw":37.6,"porod_mw_error":null,"pddf_i0":0.04817,"pddf_i0_error":null,"guinier_i0":0.04769,"guinier_i0_error":7e-05,"pddf_rg":2.85,"pddf_rg_error":null,"guinier_rg":2.761,"guinier_rg_error":0.033,"pddf_dmax":10.3,"pddf_dmax_error":null,"porod_volume":56.4,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":33,"guinier_point_last":82,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDET9_datloglog_img.png","symmetry":null,"last_modified":"2019-12-11T14:14:30.684937+01:00","bragg_peak":[]}