{"code":"SASDEU6","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Frameshift PQBP-1 mutants K192S<sup>fs*7</sup> and R153S<sup>fs*41</sup> implicated in X-linked intellectual disability form stable dimers.","publication":{"title":"Frameshift PQBP-1 mutants K192S<sup>fs*7</sup> and R153S<sup>fs*41</sup> implicated in X-linked intellectual disability form stable dimers.","author_list":"Rahman SK, Okazawa H, Chen YW","journal":"J Struct Biol","doi":"10.1016/j.jsb.2019.04.003","pmid":"30951824","published_date":"2019 Apr 2"},"status":"released","submitted_date":"2018-10-23","released_date":"2019-04-08"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEU6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEU6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEU6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEU6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEU6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEU6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEU6.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"DORIS III, DESY","city":"Hamburg","country":"Germany","beamline_name":"EMBL X33","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Polyglutamine-binding protein 1 p.Arg153Serfs*41","short_name":"R153Sfs*41","sequence":">sp|O60828|PQBP1_HUMAN Polyglutamine-binding protein 1 OS=Homo sapiens\r\nGPMPLPVALQTRLAKRGILKHLEPEPEEEIIAEDYDDDPVDYEATRLEGLPP \r\nSWYKVFDPSCGLPYYWNADTDLVSWLSPHDPNSVVTKSAKKLRSSNADAE \r\nEKLDRSHDKSDRGHDKSDRSHEKLDRGHDKSDRGHDKSDRDRERGYDKVD \r\nRESETGNGIGTAGMTRQTGKRAKNGATIAGRSWLPIPRARRQ","organism":"Homo sapiens","uniprot_code":"O60828","uniprot_range_first":1,"uniprot_range_last":154,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MPLPVALQTRLAKRGILKHLEPEPEEEIIAEDYDDDPVDYEATRLEGLPPSWYKVFDPSC\nGLPYYWNADTDLVSWLSPHDPNSVVTKSAKKLRSSNADAEEKLDRSHDKSDRGHDKSDRS\nHEKLDRGHDKSDRGHDKSDRDRERGYDKVDRERERDRERDRDRGYDKADREEGKERRHHR\nREELAPYPKSKKAVSRKDEELDPMDPSSYSDAPRGTWSTGLPKRNEAKTGADTTAAGPLF\nQQRPYPSPGAVLRANAEASRTKQQD","mw":21.914,"total_mw":43.828,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"Phosphate-buffered saline","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Polyglutamine-binding protein 1 p.Arg153Serfs*41 (PQBP-1 XLID mutant R153Sfs*41)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"London, UK","full_name":"King's College London","webpage":"https://www.kcl.ac.uk"}],"contributor_name":"Yu Wai","contributor_surname":"Chen","orcid":"http://orcid.org/0000-0001-7833-7533"}],"concentration_method":null,"concentration_unit":null,"date":"2013-02-15","storage_temperature":null,"cell_temperature":10.0,"exposure_time":0.045,"number_of_frames":null,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":1.0,"concentration_max":7.3,"sample_volume":null,"flow_rate":null,"s_min":0.081,"s_max":4.182,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEU6_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEU6_fit1_model1.pdb","model_mw":65.25,"bead_radius":3.0,"log":"https://www.sasbdb.org/media/log_files/R153S_redammin.log","symmetry":null,"comment":null,"user":342}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEU6_fit1_fixed_fit_img.png","software":"DAMMIN","chi_square_value":1.156,"p_value":0.005645,"fit_residual_plot":"SASDEU6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEU6_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/R153S_redammin.log","software_version":null,"description":""},{"models":[],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEU6_fit2_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.144,"p_value":0.023111,"fit_residual_plot":"fit4columns1868_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEU6_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_McnLiGi.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.5a","i0_calibration_standard":null,"description":"The entry displays the EOM ensemble Rg distributions and the fit to the  data of the refined ensemble. The  full EOM ensemble and log file describing the associated volume fractions is available for download in the full entry zip-archive.","experiment_description":"Synchrotron SAXS data from solutions of polyglutamine-binding protein 1 p.Arg153Serfs*41 in phosphate-buffered saline, pH 7.4 were collected on the EMBL X33 beam line at the DORIS III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 7.3 mg/ml were measured at 10°C using an X-ray exposure time of 45 ms. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":["idp","X33"],"intensity_unit":"arbitrary","experimental_mw":45.49,"experimental_mw_error":null,"guinier_i0_mw":45.49,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":2971.0,"pddf_i0_error":9.293,"guinier_i0":3006.99,"guinier_i0_error":16.14,"pddf_rg":3.61,"pddf_rg_error":0.01,"guinier_rg":3.61,"guinier_rg_error":0.19,"pddf_dmax":13.0,"pddf_dmax_error":1.0,"porod_volume":100.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":8,"guinier_point_last":106,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEU6_datloglog_img.png","symmetry":null,"last_modified":"2023-05-25T11:22:08.798758+02:00","bragg_peak":[]}