{"code":"SASDEU9","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Legionella pneumophila effector AnkX","publication":null,"status":"released","submitted_date":"2019-01-17","released_date":"2024-01-17"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEU9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEU9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEU9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEU9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEU9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEU9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEU9.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Phosphocholinase AnkX","short_name":"AnkX","sequence":"GVKIMPNLPGLYFLQAYPSEEIWRLFVDGRFWSKENGWRGYESREPGCL\r\nNAALESLCSIALQVEKSGEEFELSVDLIKRIHKKCGKKVEELQEKNPGE\r\nLRTDEPVSFGIPAGRASIKGIEEFLSLVFLTEGGAEFGPGKAGPFGPRF\r\nDKNYFKNLNPEQIPDLAKQIYFDMCKYGHSNTNHFYLAVMKNVDVYLEK\r\nITQSYNKEIKTAETLDEKLKIIVKHIRMYEVLHPFRDANGRTFVNNLLN\r\nIPLMQQGLPPATFYEPNVFDLYSAEELVVVVKEAIFNTVEIIEQSKRKT\r\nPITLYGYHSSLEEQTKFRDMLDSPSYEKIKHMDFSDLNPEKLHLKTQKC\r\nLSSLNEQYPLHRGAIYLSDPGEIKLLLSNRNESQINQQIEQGAPPIYVG\r\nKTPAHLAVISGNMAMLDELIAKKADLSLQDYDGKTALHYAAECGNMQIM\r\nGKILKVVLSQEDAIKVLNIKDNHGKTAFHYAAEFGTPELISALTTTEVI\r\nQINEPDNSGSSAITLAYKNHKLKIFDELLNSGADISDELLDAIWARKDK\r\nETLGKIIAKNEKILLNKEAFRIAISLGSVSLVKKFLRAGVDIDIPLTKD\r\nKATPLMLSINSGNPKLVSYLLKKGANTRLTDTSGNSVLHYVFYSKAENR\r\nEALANIITEKDKKLINQPNANGNPPLYNAVVVNDLKMATILLEMGARVD\r\nFEDRLGNNILHSAMRRCDLPIILDIVKKDSTLLHKRNSERRNPFHQALH\r\nEMHTFPSSKETEEIHFMNLSDLLLKEGVDLNKKDIKGKTILDIALSKQY\r\nFHLCVKLMKAGAHTNISSPSKFLKNSDANSILERPFKFKNDLKKELDNN\r\nPLIAMAQINDLYVQIKNNRIRTPTGYAPKEGVSFFKGKSNDAKAHDEVL\r\nSVLKELYDSKLTEMLGNLPGEGLEEIKRSQKFFDGELKLLIKNQDISRK\r\nVDKKSIQEAVGTSLKLKW","organism":"Legionella pneumophila","uniprot_code":"Q5ZXN6","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MVKIMPNLPGLYFLQAYPSEEIWRLFVDGRFWSKENGWRGYESREPGCLNAALESLCSIA\nLQVEKSGEEFELSVDLIKRIHKKCGKKVEELQEKNPGELRTDEPVSFGIPAGRASIKGIE\nEFLSLVFLTEGGAEFGPGKAGPFGPRFDKNYFKNLNPEQIPDLAKQIYFDMCKYGHSNTN\nHFYLAVMKNVDVYLEKITQSYNKEIKTAETLDEKLKIIVKHIRMYEVLHPFRDANGRTFV\nNNLLNILLMQQGLPPATFYEPNVFDLYSAEELVVVVKEAIFNTVEIIEQSKRKTPITLYG\nYHSSLEEQTKFRDMLDSPSYEKIKHMDFSDLNPEKLHLKTQKCLSSLNEQYPLHRGAIYL\nSDPGEIKLLLSNRNESQINQQIEQGAPPIYVGKTPAHLAVISGNMAMLDELIAKKADLSL\nQDYDGKTALHYAAECGNMQIMGKILKVVLSQEDAIKVLNIKDNHGKTAFHYAAEFGTPEL\nISALTTTEVIQINEPDNSGSSAITLAYKNHKLKIFDELLNSGADISDELLDAIWARKDKE\nTLGKIIAKNEKILLNKEAFRIAISLGSVSLVKKFLRAGVDIDIPLTKDKATPLMLSINSG\nNPKLVSYLLKKGANTRLTDTSGNSVLHYVFYSKAENREALANIITEKDKKLINQPNANGN\nPPLYNAVVVNDLKMATILLEMGARVDFEDRLGNNILHSAMRRCDLPIILDIVKKDSTLLH\nKRNSERRNPFHQALHEMHTFPSSKETEEIHFMNLSDLLLKEGVDLNKKDIKGKTILDIAL\nSKQYFHLCVKLMKAGAHTNISSPSKFLKNSDANSILERPFKFKNDLKKELDNNPLIAMAQ\nINDLYVQIKNNRIRTPTGYAPKEGVSFFKGKSNDAKAHDEVLSVLKELYDSKLTEMLGNL\nPGEGLEEIKRSQKFFDGELKLLIKNQDISRKVDKKSIQEAVGTSLKLKW","mw":107.2,"total_mw":107.2,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Legionella pneumophila Phosphocholinase AnkX","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Cachan, France","full_name":"CNRS UMR8113, ENS Paris-Saclay","webpage":"http://lbpa.ens-paris-saclay.fr/"}],"contributor_name":"Wenhua","contributor_surname":"Zhang","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2017-10-20","storage_temperature":null,"cell_temperature":null,"exposure_time":null,"number_of_frames":null,"wavelength":0.09919,"sample_detector_distance":null,"concentration_min":null,"concentration_max":8.0,"sample_volume":null,"flow_rate":null,"s_min":0.179,"s_max":2.06,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEU9_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEU9_fit1_model1.pdb","model_mw":null,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/AnkX.log","symmetry":"P1","comment":null,"user":84}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEU9_fit1_fixed_fit_img.png","software":"GASBOR","chi_square_value":1.05,"p_value":0.0,"fit_residual_plot":"SASDEU9_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEU9_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/AnkX.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"X-ray exposure time = UNKNOWN. Sample temperature = UNKNOWN.","experiment_description":"Synchrotron SAXS data from solutions of phosphocholinase AnkX in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the BM29 camera at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.00 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":109.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":90.0,"porod_mw_error":null,"pddf_i0":86.76,"pddf_i0_error":null,"guinier_i0":86.33,"guinier_i0_error":0.1,"pddf_rg":4.324,"pddf_rg_error":null,"guinier_rg":4.25,"guinier_rg_error":0.03,"pddf_dmax":14.7,"pddf_dmax_error":null,"porod_volume":145.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":3,"guinier_point_last":26,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEU9_datloglog_img.png","symmetry":null,"last_modified":"2024-02-21T18:33:53.450280+01:00","bragg_peak":[]}