{"code":"SASDEV4","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Structural basis for activation of plasma-membrane Ca<sup>2+</sup>-ATPase by calmodulin.","publication":{"title":"Structural basis for activation of plasma-membrane Ca<sup>2+</sup>-ATPase by calmodulin.","author_list":"Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H","journal":"Commun Biol","doi":"10.1038/s42003-018-0203-7","pmid":"30511020","published_date":"2018"},"status":"released","submitted_date":null,"released_date":"2018-12-03"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEV4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEV4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEV4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEV4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Membrane scaffold protein 1D1 (deuterated, 75%)","short_name":"d75-msp1d1","sequence":"GHHHHHHHDYDIPTTENLYFQGSTFSKLREQLGPVTQEFWDNLEKETEGLRQEM\r\nSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQE\r\nKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEY\r\nHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":null,"mw":24.661,"total_mw":49.322,"number_molecules":0,"complex_state":false,"deuteration":75.0,"molecule_source":"biological","molecule_description":null},{"long_name":"1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl)","short_name":"d(78,92)-POPC","sequence":"C42H82NO8P","organism":"Escherichia coli","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"unknown","molecular_type":"other","uniprot_sequence":null,"mw":0.76,"total_mw":0.76,"number_molecules":124,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Calcium-transporting ATPase 8, plasma membrane-type","short_name":"ACA8","sequence":"HHHHHHHHENLYFQGS\r\nMTSLLKSSPGRRRGGDVESGKSEHADSDSDTFYIPSKNASIERLQQWRKAALVLNASRRF\r\nRYTLDLKKEQETREMRQKIRSHAHALLAANRFMDMGRESGVEKTTGPATPAGDFGITPEQ\r\nLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKGKG\r\nFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVSDY\r\nKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGHSL\r\nALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISEDNG\r\nEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGHVI\r\nDDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATTIC\r\nSDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLVVEGISQNTTGSIFVPEGGGDLEY\r\nSGSPTEKAILGWGVKLGMNFETARSQSSILHAFPFNSEKKRGGVAVKTADGEVHVHWKGA\r\nSEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTYEAEKVPTGE\r\nELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNVQTARAIALEC\r\nGILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVV\r\nAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANI\r\nQKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLM\r\nGRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTII\r\nFNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEFLGKFASTTKL\r\nNWKQWLICVGIGVISWPLALVGKFIPVPAAPISNKLKVLKFWGKKKNSSGEGSL","organism":"Arabidopsis thaliana","uniprot_code":"Q9LF79","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MTSLLKSSPGRRRGGDVESGKSEHADSDSDTFYIPSKNASIERLQQWRKAALVLNASRRF\nRYTLDLKKEQETREMRQKIRSHAHALLAANRFMDMGRESGVEKTTGPATPAGDFGITPEQ\nLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKGKG\nFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVSDY\nKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGHSL\nALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISEDNG\nEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGHVI\nDDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATTIC\nSDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLVVEGISQNTTGSIFVPEGGGDLEY\nSGSPTEKAILGWGVKLGMNFETARSQSSILHAFPFNSEKKRGGVAVKTADGEVHVHWKGA\nSEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTYEAEKVPTGE\nELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNVQTARAIALEC\nGILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVV\nAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANI\nQKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLM\nGRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTII\nFNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEFLGKFASTTKL\nNWKQWLICVGIGVISWPLALVGKFIPVPAAPISNKLKVLKFWGKKKNSSGEGSL","mw":118.209,"total_mw":118.209,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"ACA8 protein (apo) in stealth nanodisc","ext_coefficient":null,"contrast":1.808,"specific_vol":0.816,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Haydyn","contributor_surname":"Mertens","orcid":null}],"concentration_method":"A280","concentration_unit":null,"date":"2017-09-08","storage_temperature":10.0,"cell_temperature":10.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":3.3,"concentration_min":1.0,"concentration_max":8.0,"sample_volume":null,"flow_rate":null,"s_min":0.025,"s_max":5.064,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"ACA8 protein (apo) encapsulated in a partially deuterated \"stealth-nanodisc\": (sND).","experiment_description":null,"tags":[],"intensity_unit":"1/cm","experimental_mw":259.0,"experimental_mw_error":25.0,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":391.0,"porod_mw_error":40.0,"pddf_i0":0.093,"pddf_i0_error":0.001,"guinier_i0":0.094,"guinier_i0_error":0.001,"pddf_rg":5.38,"pddf_rg_error":0.01,"guinier_rg":5.3,"guinier_rg_error":0.01,"pddf_dmax":20.0,"pddf_dmax_error":0.5,"porod_volume":626.0,"porod_volume_error":50.0,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":32,"guinier_point_last":78,"pddf_point_first":32,"pddf_point_last":1100,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEV4_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}