{"code":"SASDEV5","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Solution structures of long-acting insulin analogues and their complexes with albumin.","publication":{"title":"Solution structures of long-acting insulin analogues and their complexes with albumin.","author_list":"Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P","journal":"Acta Crystallogr D Struct Biol","doi":"10.1107/S2059798318017552","pmid":"30950398","published_date":"2019 Mar 1"},"status":"released","submitted_date":"2018-10-03","released_date":"2019-04-07"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEV5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEV5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEV5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEV5.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"MAX IV","city":"Lund","country":"Sweden","beamline_name":"I911-4","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":true,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Insulin detemir","short_name":null,"sequence":"GIVEQCCTSICSLYQLENYCN\r\nFVNQHLCGSHLVEALYLVCGERGFFYTPK","organism":null,"uniprot_code":"P01308","uniprot_range_first":25,"uniprot_range_last":110,"oligomerization":"18-mer","molecular_type":"protein","uniprot_sequence":"MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAED\nLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN","mw":5.9,"total_mw":106.2,"number_molecules":18,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"5.0 mM Na2HPO4, 13.1 mM m-cresol, 15.1 mM phenol, 173.7 mM glycerol, 20.0 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Insulin detemir tri-hexamer","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Lyngby, Denmark","full_name":"Technical University of Denmark","webpage":"http://www.dtu.dk"}],"contributor_name":"Line Abildgaard","contributor_surname":"Ryberg","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2015-09-25","storage_temperature":null,"cell_temperature":20.0,"exposure_time":30.0,"number_of_frames":4,"wavelength":0.091,"sample_detector_distance":1.962,"concentration_min":null,"concentration_max":2.5,"sample_volume":null,"flow_rate":null,"s_min":0.085,"s_max":5.183,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEV5_fit2_model1_img.png","software":"SASREF CV","pdb_link":[{"pdb_code":"1trz","ext_code":"1TRZ","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEV5_fit2_model1.pdb","model_mw":102.8,"bead_radius":null,"log":null,"symmetry":"P1","comment":null,"user":329}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/T3R3-1000_NkO93Be_fit_img.png","software":"CRYSOL","chi_square_value":1.162,"p_value":0.0,"fit_residual_plot":"T3R3-1000_NkO93Be_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEV5_fit2.fit","fit_log":null,"software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEV5_fit3_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEV5_fit3_model1.pdb","model_mw":78.2,"bead_radius":2.7,"log":null,"symmetry":"P1","comment":null,"user":329}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/dammif_fit_img.png","software":"DAMMIF","chi_square_value":0.802,"p_value":6e-06,"fit_residual_plot":"dammif_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEV5_fit3.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of insulin detemir tri-hexamer in 5.0 mM Na2HPO4, 13.1 mM m-cresol, 15.1 mM phenol, 173.7 mM glycerol, 20.0 mM NaCl, pH 7.4 were collected on the I911-4 beam line at the MAX IV storage ring (Lund, Sweden) using a Dectris Pilatus 1M detector at a sample-detector distance of 2.0 m and at a wavelength of λ = 0.091 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.50 mg/ml was measured at 20°C. Four successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":100.3,"experimental_mw_error":null,"guinier_i0_mw":100.3,"guinier_i0_mw_error":null,"porod_mw":91.5,"porod_mw_error":null,"pddf_i0":0.07362,"pddf_i0_error":null,"guinier_i0":0.072,"guinier_i0_error":null,"pddf_rg":3.35,"pddf_rg_error":null,"guinier_rg":3.26,"guinier_rg_error":null,"pddf_dmax":11.32,"pddf_dmax_error":null,"porod_volume":137.2,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":null,"guinier_point_last":null,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEV5_datloglog_img.png","symmetry":null,"last_modified":"2020-11-27T11:56:59.741576+01:00","bragg_peak":[]}