{"code":"SASDEV7","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility.","publication":{"title":"The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility.","author_list":"Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV","journal":"Nat Microbiol","doi":"10.1038/s41564-019-0622-3","pmid":"31844299","published_date":"2019 Dec 16"},"status":"released","submitted_date":"2018-11-12","released_date":"2019-11-14"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEV7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEV7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEV7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEV7.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 2M","resolution":172.0},"name":"Advanced Light Source (ALS)","city":"Berkeley, CA","country":"USA","beamline_name":"12.3.1 (SIBYLS)","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Stator protein FlaG soluble domain","short_name":"sFlaG","sequence":"MGSSHHHHHHSQDPNSISTTMSSYSIQQSQKMLTQLQIDYATNTSSNTVVAYLHNVGETTISYLQNSVVYFGPNGQLQPVGYNSGSSPYWTVTSNSLQPGSVVKIIIYLSSPLSSNQYYTIQIVTPNGYTVSYMF","organism":"Sulfolobus acidocaldarius","uniprot_code":"Q4J9K7","uniprot_range_first":32,"uniprot_range_last":151,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MVSEVISETIMIIVSVTLIGVLAGTVFSVASSISTTMSSYSIQQSQKMLTQLQIDYATNT\nSSNTVVAYLHNVGETTISYLQNSVVYFGPNGQLQPVGYNSGSSPYWTVTSNSLQPGSVVK\nIIIYLSSPLSSNQYYTIQIVTPNGYTVSYMFKVS","mw":14.932,"total_mw":29.864,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Conserved flagellar protein FlaF-I96Y soluble domain","short_name":"sFlaF-I96Y","sequence":"MGSSHHHHHHSQDPNSNQAQELNHELELEQLETKITVSSVSLTGSTLNVVLENNGSTNLYDFQGFSVIVQYYANISNYSTFNLSLYNYTKNSNPSPYYWTINTPLLAPGSQATLTIILPYPPYPNTQATVVIVTNYGPSVIWRGSL","organism":"Sulfolobus acidocaldarius","uniprot_code":"Q4J9K8","uniprot_range_first":35,"uniprot_range_last":164,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MGVSQTLAFILITFIGVSFGIVLLSAYFKSQQILNQAQELNHELELEQLETKITVSSVSL\nTGSTLNVVLENNGSTNLYDFQGFSVIVQYYANISNISTFNLSLYNYTKNSNPSPYYWTIN\nTPLLAPGSQATLTIILPYPPYPNTQATVVIVTNYGPSVIWRGSL","mw":16.289,"total_mw":32.578,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":3.0,"deuteration":null},"purity_method":null,"name":"Stator protein complex FlaG/FlaF-I96Y","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Texas, USA","full_name":"MD Anderson Cancer Center","webpage":"https://www.mdanderson.org/"}],"contributor_name":"Chi-Lin","contributor_surname":"Tsai","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-11-10","storage_temperature":null,"cell_temperature":10.0,"exposure_time":0.3,"number_of_frames":33,"wavelength":0.127,"sample_detector_distance":1.5,"concentration_min":1.5,"concentration_max":3.0,"sample_volume":null,"flow_rate":null,"s_min":0.125,"s_max":3.698,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEV7_fit1_model1_img.png","software":"BILBOMD","pdb_link":[{"pdb_code":"5tug","ext_code":"5TUG","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEV7_fit1_model1.pdb","model_mw":187.9,"bead_radius":null,"log":null,"symmetry":null,"comment":"The His-tag is modeled by Modeller","user":321}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEV7_fit1_fixed_fit_img.png","software":"FoXS","chi_square_value":10.038432,"p_value":0.0,"fit_residual_plot":"SASDEV7_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEV7_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.5a","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the stator protein complex FlaG/FlaF-I96Y in 25 mM citric acid/sodium citrate, 150mM NaCl, 3% glycerol, pH 3 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.5 and 3 mg/ml were measured at 10°C. 33 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"1/cm","experimental_mw":50.85,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":56.0,"porod_mw_error":null,"pddf_i0":208.5,"pddf_i0_error":null,"guinier_i0":208.48,"guinier_i0_error":0.22,"pddf_rg":2.67,"pddf_rg_error":null,"guinier_rg":2.675,"guinier_rg_error":0.004,"pddf_dmax":8.2,"pddf_dmax_error":null,"porod_volume":90.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":62,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEV7_datloglog_img.png","symmetry":null,"last_modified":"2019-12-19T18:54:58.540247+01:00","bragg_peak":[]}