{"code":"SASDEV8","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations.","publication":{"title":"Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations.","author_list":"Gijsbers A, Montagut DC, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N","journal":"Int J Mol Sci","doi":"10.3390/ijms19124012","pmid":"30545121","published_date":"2018 Dec 12"},"status":"released","submitted_date":"2018-12-06","released_date":"2018-12-14"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEV8.dat","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEV8.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV8_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV8_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEV8_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEV8_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEV8.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Ribosome maturation protein SDO1","short_name":"SDO1","sequence":"MPINQPSGQIKLTNVSLVRLKKARKRFEVACYQNKVQDYRKGIEKDLDEVLQIHQVFMNV\r\n SKGLVANKEDLQKCFGTTNVDDVIEEIMHKGEIQLSEKERQLMLNKVNNEMLTIVSAKCI\r\n NPVSKKRYPPTMIHKALQELKFSPVINKPAKLQALEAIKLLVSKQIIPIVRAKMKVKVAI\r\n SEPSRQPELIEKISKLIASSPGESTKPELDPWTCTGLIDPVNYRDLMTLCDKKGTVQVLD\r\n MAVIDNTTHN","organism":"Saccharomyces cerevisiae","uniprot_code":"Q07953","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MPINQPSGQIKLTNVSLVRLKKARKRFEVACYQNKVQDYRKGIEKDLDEVLQIHQVFMNV\nSKGLVANKEDLQKCFGTTNVDDVIEEIMHKGEIQLSEKERQLMLNKVNNEMLTIVSAKCI\nNPVSKKRYPPTMIHKALQELKFSPVINKPAKLQALEAIKLLVSKQIIPIVRAKMKVKVAI\nSEPSRQPELIEKISKLIASSPGESTKPELDPWTCTGLIDPVNYRDLMTLCDKKGTVQVLD\nMAVIDNTTHN","mw":28.283,"total_mw":28.283,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2.","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":null,"deuteration":null},"purity_method":null,"name":"Shwachman-Bodian-Diamond Syndrome protein (yeast SDO1)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Bari, Italy","full_name":"CNR - Institute of Crystallography, Bari, Italy","webpage":"http://www.ba.ic.cnr.it"}],"contributor_name":"Dritan","contributor_surname":"Siliqi","orcid":"https://orcid.org/0000-0003-1415-8725"}],"concentration_method":null,"concentration_unit":null,"date":"2017-09-21","storage_temperature":null,"cell_temperature":20.0,"exposure_time":3.0,"number_of_frames":580,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.158,"s_max":3.609,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEV8_fit1_model1_img.png","software":"MultiFoXS","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEV8_fit1_model1.pdb","model_mw":28.29,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":396},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEV8_fit1_model2_img.png","software":"MultiFoXS","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEV8_fit1_model2.pdb","model_mw":28.29,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":396},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEV8_fit1_model3_img.png","software":"MultiFoXS","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEV8_fit1_model3.pdb","model_mw":28.29,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":396}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/multi_state_model_3_1_1_fitfoxs_img.png","software":"FoXS","chi_square_value":1.292325,"p_value":0.426822,"fit_residual_plot":"multi_state_model_3_1_1_fitfoxsresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEV8_fit1.dat","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ScÅtter","pddf_software_version":null,"i0_calibration_standard":null,"description":"SEC-SAXS  was performed at 20°C using the following parameters: Column: Shodex KW-403  ; Flow rate: 0.408 mL/min; Total acquisition time: 1740s; Sample injection concentration: 10 mg/mL; Injection volume: 45μL.","experiment_description":"Synchrotron SAXS data from solutions of Shwachman-Bodian-Diamond Syndrome protein (yeast SDO1) in 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl and 5 mM MgCl2., pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the B21 camera at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.0 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data obtained through the sample elution peak (collected as 580 successive 3 second frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":null,"experimental_mw":29.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.08047757,"pddf_i0_error":null,"guinier_i0":0.011,"guinier_i0_error":4e-05,"pddf_rg":2.64,"pddf_rg_error":null,"guinier_rg":2.74,"guinier_rg_error":0.05,"pddf_dmax":8.5,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":144,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEV8_datloglog_img.png","symmetry":null,"last_modified":"2024-07-15T11:06:06.222393+02:00","bragg_peak":[]}