{"code":"SASDEW9","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.","publication":{"title":"Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.","author_list":"Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG","journal":"Structure","doi":"10.1016/j.str.2019.09.007","pmid":"31601460","published_date":"2019 Oct 5"},"status":"released","submitted_date":"2019-01-17","released_date":"2019-10-14"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEW9.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEW9.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEW9_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEW9_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEW9_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEW9_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEW9.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Cytohesin-2 ARF nucleotide-binding site opener","short_name":"ARNO","sequence":"MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQRNRKMAMGRK\r\nKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREELNLAVLHAFVDLHEFTDLNL\r\nVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQSTDTCYVLSFAVIMLNTSLHNPNVRDKPGL\r\nERFVAMNRGINEGGDLPEELLRNLYDSIRNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRR\r\nWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGN\r\nHMVYRISAPTQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP","organism":"Homo sapiens","uniprot_code":"Q99418","uniprot_range_first":1,"uniprot_range_last":400,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MEDGVYEPPDLTPEERMELENIRRRKQELLVEIQRLREELSEAMSEVEGLEANEGSKTLQ\nRNRKMAMGRKKFNMDPKKGIQFLVENELLQNTPEEIARFLYKGEGLNKTAIGDYLGEREE\nLNLAVLHAFVDLHEFTDLNLVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCLCNPGVFQ\nSTDTCYVLSFAVIMLNTSLHNPNVRDKPGLERFVAMNRGINEGGDLPEELLRNLYDSIRN\nEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEP\nRGIIPLENLSIREVDDPRKPNCFELYIPNNKGQLIKACKTEADGRVVEGNHMVYRISAPT\nQEEKDEWIKSIQAAVSVDPFYEMLAARKKRISVKKKQEQP","mw":46.6,"total_mw":93.2,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Cytohesin-2; ARF nucleotide-binding site opener, ARNO","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Cachan, France","full_name":"CNRS UMR8113, ENS Paris-Saclay","webpage":"http://lbpa.ens-paris-saclay.fr/"}],"contributor_name":"Wenhua","contributor_surname":"Zhang","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-06-23","storage_temperature":15.0,"cell_temperature":15.0,"exposure_time":1.0,"number_of_frames":1500,"wavelength":0.993,"sample_detector_distance":null,"concentration_min":null,"concentration_max":10.0,"sample_volume":null,"flow_rate":null,"s_min":0.072,"s_max":4.952,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEW9_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEW9_fit1_model1.pdb","model_mw":null,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/GS-4_1.log","symmetry":null,"comment":null,"user":84}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/GS-4_1_fit_img.png","software":"GASBOR","chi_square_value":1.45,"p_value":0.0,"fit_residual_plot":"GS-4_1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEW9_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/GS-4_1.log","software_version":null,"description":null}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the cytohesin-2; ARF nucleotide-binding site opener, ARNO, in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a wavelength of λ = 0.993 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 15°C. 1500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":94.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":90.0,"porod_mw_error":null,"pddf_i0":44.95,"pddf_i0_error":null,"guinier_i0":43.99,"guinier_i0_error":0.067,"pddf_rg":5.107,"pddf_rg_error":null,"guinier_rg":4.75,"guinier_rg_error":0.1,"pddf_dmax":19.65,"pddf_dmax_error":null,"porod_volume":145.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":5,"guinier_point_last":43,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEW9_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}