{"code":"SASDEY6","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"Structural Characterisation of the Arabidopsis thaliana APC4","publication":null,"status":"released","submitted_date":"2018-10-29","released_date":"2020-12-12"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDEY6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDEY6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEY6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEY6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDEY6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDEY6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDEY6.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 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4","short_name":"Apc4","sequence":">APC4-strep\r\nMSEMASDEEENIIPFQLQFDKPIPFQIKIAEWNPEKDLLAMVTEDSKILLHRFNWQRLWTISPGKPVTSLCWRPDGKAIAVGLEDGTISLHDVENGKLLRNLKPHDVAVVCLNWEEDGQSNTDESGNFSVYEDRTSRFFPPAPRPPKMPGLVAGDSSFMDDGEDSLAELSNTSFRKFNILCTGDRDGNICFSIFGIFQIGKINIHELSLPVPHLDEHASCKLFNASIYKVALSKDLCRLVVMCTGELKDCDIKPREEKINVQDLPGLHCLAMDTSIFWKRKYELHQVAQQASNIEDLTEVIRASLSVMNKQWADAMKTFHEKFHSLSTLIIDNGLESSPQEEFLSLLGGARISPALNQFLVNSLGEVGVKRVLKSVCGTGKELQQVVLDHLQPAAEIIGFRIGELRGLSRWRARYQGIGLDEMLLNEATENTGLLLVQVQRFMMVLSSVVQQFSNFFNWLVRSIKYLMQEPNDQLLSYNSELLVVFLKFLYDQDPVKDLLELSEAGDDIEIDLKTIGRVKELLQFGGFSECDFLQRTLAKEFQHMESSFKMAFQMPFTTISRKISCMKLLPLCPLQLSTTQTPTTIPMSLSFYKNELSDDTPCQSGYTDYISFQVPDETFPEISNCIGIAKGYKQNSNNEKNGYTSLEAVLLSVPNGYTCVDLSLYKDKELVLLLNKTNTDSEGSGEACMMVVQTGDLAFISISGSSSLNQWELEDLKGSIVNLEMENEKVRKVPHSVIAPLAVSASRGVACVFAERRRALVYILEEDEDEEISDEKDYDIPTTENLYFQGSAWSHPQFEKGGGSGGGSGGGSWSHPQFEK","organism":"Arabidopsis 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mM Tris 150 mM NaCl 1 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Arabidopsis thaliana anaphase promoting complex/cyclosome subunit 4 (APC4-strep)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"VUB","address":"Pleinlaan 2 1050 Brussel ","full_name":"Vrije Universiteit Brussel","webpage":"http://www.vub.ac.be/"}],"contributor_name":"Steven","contributor_surname":"De Gieter","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2017-03-06","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":69,"wavelength":0.099,"sample_detector_distance":2.867,"concentration_min":8.1,"concentration_max":8.1,"sample_volume":null,"flow_rate":null,"s_min":0.036,"s_max":3.51,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEY6_fit1_model1_img.png","software":"SREFLEX","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEY6_fit1_model1.pdb","model_mw":184.1,"bead_radius":null,"log":null,"symmetry":"P2","comment":null,"user":384}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEY6_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":3.102,"p_value":0.0,"fit_residual_plot":"SASDEY6_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEY6_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/rc0100.log","software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDEY6_fit2_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDEY6_fit2_model1.pdb","model_mw":160.835,"bead_radius":3.9,"log":null,"symmetry":"P2","comment":null,"user":384}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEY6_fit2_fixed_fit_img.png","software":"DAMMIF","chi_square_value":0.788,"p_value":0.000665,"fit_residual_plot":"SASDEY6_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEY6_fit2.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of anaphase promoting complex/cyclosome subunit 4 in 10 mM Tris ,150 mM NaCl, 1 mM TCEP, pH 8 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed using the following parameters: Column type: Shodex KW404-4F; Injection volume: 50 µl; Sample injection concentration: 8.1 mg/ml. 69 successive 1 second frames were collected through the elution peak (measured at 20°C). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":192.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":162.0,"porod_mw_error":null,"pddf_i0":50.1,"pddf_i0_error":null,"guinier_i0":49.9,"guinier_i0_error":0.042,"pddf_rg":4.702,"pddf_rg_error":null,"guinier_rg":4.64,"guinier_rg_error":0.01,"pddf_dmax":16.2,"pddf_dmax_error":null,"porod_volume":259.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":19,"guinier_point_last":53,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEY6_datloglog_img.png","symmetry":null,"last_modified":"2020-12-12T16:01:14.893165+01:00","bragg_peak":[]}