{"code":"SASDEY9","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"Structure of a collagen VI α3 chain VWA domain array: adaptability and functional implications of myopathy causing mutations","publication":{"title":"Structure of a collagen VI α3 chain VWA domain array: adaptability and functional implications of myopathy causing mutations","author_list":"Solomon-Degefa H, Gebauer J, Jeffries C, Freiburg C, Meckelburg P, Bird L, Baumann U, Svergun D, Owens R, Werner J, Behrmann E, Paulsson M, Wagener R","journal":"Journal of Biological Chemistry","doi":"10.1074/jbc.RA120.014865","pmid":null,"published_date":"2020 Jul 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using the crystal structure of N5 in combination with an ITASSER homology model of N4","user":9}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEY9_fit3_fixed_fit_img.png","software":"CRYSOL","chi_square_value":1.018,"p_value":0.180377,"fit_residual_plot":"SASDEY9_fit3_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEY9_fit3.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/XF00.log","software_version":"2.8.3","description":""},{"models":[],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDEY9_fit4_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":1.037,"p_value":0.1753,"fit_residual_plot":"SASDEY9_fit4_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDEY9_fit4.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the collagen VI N5N4 double-domain fragment in 20 mM Tris, pH 7.4, 150mM NaCl 3% v/v glycerol, were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) equipped with a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20°C using the following parameters: Column: GE-Healthcare S75 Increase 10/300 analytical SEC column; Flow rate: 0.6 mL/min; Sample injection concentration: 12.0 mg/mL; Injection volume: 55 μL. Total X-ray acquisition time = 2400 s. The data were collected through the SEC peak of the protein as a series of 18 x 1 second exposures and processed using CHROMIXS (Panjkovich & Svergun, 2018 Bioinformatics 34(11):1944-1946) that was also used to select an appropriate matched solvent-blank. Parallel inline multi-angle laser and dynamic light scattering with refractive index measurements (SEC-MALLS/DLS/RI) were performed using a Wyatt Mini-Dawn TREOS with an in-built quasi elastic light scattering (QELS) module coupled to an OptiLab T-Rex refractometer. The quoted experiential MW for this entry is derived from MALLS. Concentration-independent MW estimates obtained directly from the SAXS data using DATMW (Hajizadeh et al., 2018 Scientific Reports 8, 7204), as part of CHROMIXS/ATSAS 2.8.3, as well as the SEC-MALLS/DLS/RI data and additional modelling files, including ITASSER homology modelling (Yang et al., 2015 Nature Methods 12, 7-8) are included in the full-entry zip archive.","tags":[],"intensity_unit":"arbitrary","experimental_mw":43.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":41.0,"porod_mw_error":null,"pddf_i0":287.2,"pddf_i0_error":null,"guinier_i0":286.77,"guinier_i0_error":0.42,"pddf_rg":2.895,"pddf_rg_error":null,"guinier_rg":2.85,"guinier_rg_error":0.01,"pddf_dmax":9.85,"pddf_dmax_error":null,"porod_volume":66.2,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":122,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDEY9_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:55:24.740692+02:00","bragg_peak":[]}