{"code":"SASDF29","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.","publication":{"title":"Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.","author_list":"Yadav DK, Zigáčková D, Zlobina M, Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkz1163","pmid":"31875226","published_date":"2019 Dec 25"},"status":"released","submitted_date":"2019-07-08","released_date":"2019-11-29"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDF29.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF29.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF29_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF29_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF29_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF29_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF29.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"CEITEC","city":"Brno","country":"Czech Republic","beamline_name":"Rigaku BioSAXS-1000","beam_geometry":"","type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4","short_name":"STAU1-RBD4","sequence":"GSHMNPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFK","organism":"Homo sapiens","uniprot_code":"O95793","uniprot_range_first":286,"uniprot_range_last":355,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSQVQVQVQNPSAALSGSQILNKNQSLLSQPLMSIPSTTSSLPSENAGRPIQNSALPSAS\nITSTSAAAESITPTVELNALCMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPF\nPVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEEN\nLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKN\nAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKE\nKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQ\nPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPM\nVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGH\nVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVES\nCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC","mw":8.377,"total_mw":8.377,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"ADP-ribosylation factor1 - short","short_name":"ARF1 RNA - short","sequence":"GGCAGAAGCUGCCUCUUCGGAGGCAGUUUCUGCC","organism":"Homo sapiens","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"RNA","uniprot_sequence":null,"mw":10.962,"total_mw":10.962,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.8,"deuteration":null},"purity_method":null,"name":"Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"CEITEC - Central European Institute of Technology, Masaryk University","webpage":"https://www.ceitec.eu/"}],"contributor_name":"Tomas","contributor_surname":"Klumpler","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2015-12-10","storage_temperature":20.0,"cell_temperature":35.0,"exposure_time":600.0,"number_of_frames":6,"wavelength":0.154,"sample_detector_distance":0.48,"concentration_min":null,"concentration_max":6.5,"sample_volume":null,"flow_rate":null,"s_min":0.483,"s_max":3.013,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDF29_fit1_model1_img.png","software":"Other","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":"CYANA","model_data":"https://www.sasbdb.org/media/pdb_file/SASDF29_fit1_model1.pdb","model_mw":384.2,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":195}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDF29_fit1_fixed_fit_img.png","software":"CRYSOL","chi_square_value":1.167,"p_value":1e-06,"fit_residual_plot":"SASDF29_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDF29_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/dsRBD4_hARF-for-deposition-2018-11-19_noH-100.log","software_version":"2.8.3","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 in 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol, pH 6.8 were collected using a Rigaku BioSAXS-1000 instrument (CEITEC; Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.50 mg/ml was measured at 35°C. Six successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":15.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":12.938,"porod_mw_error":null,"pddf_i0":0.4536,"pddf_i0_error":null,"guinier_i0":0.44,"guinier_i0_error":0.00043,"pddf_rg":1.81,"pddf_rg_error":null,"guinier_rg":1.723,"guinier_rg_error":0.073,"pddf_dmax":5.98,"pddf_dmax_error":null,"porod_volume":20.7,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":19,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF29_datloglog_img.png","symmetry":null,"last_modified":"2020-02-12T18:29:36.078856+01:00","bragg_peak":[]}