{"code":"SASDF42","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative.","publication":{"title":"Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative.","author_list":"Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P","journal":"Biochemistry","doi":"10.1021/acs.biochem.0c00019","pmid":"32208682","published_date":"2020 Mar 25"},"status":"released","submitted_date":"2019-01-28","released_date":"2020-03-31"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDF42.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF42.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF42_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF42_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF42_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF42_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF42.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 100K","resolution":172.0},"name":"Novo Nordisk A/S","city":"Maaloev","country":"Denmark","beamline_name":"Rigaku BioSAXS-2000","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Human serum albumin","short_name":"HSA","sequence":"DAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPFEDHVKLVNEVTEFAKTCVADESAE\r\nNCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEPERNECFLQHKDDNPNLPRLVRPEV\r\nDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLFFAKRYKAAFTECCQAADKAACLLP\r\nKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAVARLSQRFPKAEFAEVSKLVTDLTK\r\nVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPA\r\nDLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKC\r\nCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVST\r\nPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTES\r\nLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKAT\r\nKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL","organism":"Homo sapiens","uniprot_code":"P02768","uniprot_range_first":25,"uniprot_range_last":609,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MKWVTFISLLFLFSSAYSRGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPF\nEDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEP\nERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLF\nFAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAV\nARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLK\nECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYAR\nRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFE\nQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVV\nLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTL\nSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLV\nAASQAALGL","mw":66.472,"total_mw":66.472,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Somapacitan","short_name":null,"sequence":"FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSCVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF","organism":"Homo sapiens","uniprot_code":"P01241","uniprot_range_first":27,"uniprot_range_last":217,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MATGSRTSLLLAFGLLCLPWLQEGSAFPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEA\nYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLR\nSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDD\nALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF","mw":22.119,"total_mw":44.238,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"100 mM MES, 140 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.5,"deuteration":null},"purity_method":null,"name":"Human serum albumin in complex with somapacitan","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Denmark","full_name":"Novo Nordisk A/S","webpage":"https://www.novonordisk.com/"}],"contributor_name":"Eva","contributor_surname":"Johansson","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2015-09-04","storage_temperature":null,"cell_temperature":25.0,"exposure_time":3600.0,"number_of_frames":6,"wavelength":0.1542,"sample_detector_distance":null,"concentration_min":null,"concentration_max":6.3,"sample_volume":null,"flow_rate":null,"s_min":0.037,"s_max":6.45,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDF42_fit1_model1_img.png","software":"SASREF","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDF42_fit1_model1.pdb","model_mw":108.3,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/15Anew.log","symmetry":null,"comment":null,"user":408}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDF42_fit1_fixed_fit_img.png","software":"SASREF","chi_square_value":2.1025,"p_value":0.002492,"fit_residual_plot":"SASDF42_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDF42_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.5a","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of HSA in complex with somapacitan in 100 mM MES, 140 mM NaCl, pH 6.5 were collected on a Rigaku BioSAXS-2000 instrument at Novo Nordisk A/S (Maaloev, Denmark) using a Pilatus 100K detector at a wavelength of λ = 0.1542 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.30 mg/ml was measured at 25°C. Six successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":113.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":126.0,"porod_mw_error":null,"pddf_i0":6.834,"pddf_i0_error":null,"guinier_i0":6.843,"guinier_i0_error":0.02,"pddf_rg":4.12,"pddf_rg_error":null,"guinier_rg":4.06,"guinier_rg_error":0.02,"pddf_dmax":13.92,"pddf_dmax_error":null,"porod_volume":202.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":6,"guinier_point_last":20,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF42_datloglog_img.png","symmetry":null,"last_modified":"2020-11-27T12:03:56.572790+01:00","bragg_peak":[]}