{"code":"SASDF48","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Molecular basis of F-actin regulation and sarcomere assembly via myotilin","publication":{"title":"Molecular basis of F-actin regulation and sarcomere assembly via myotilin","author_list":"Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L","journal":"PLOS Biology","doi":"10.1371/journal.pbio.3001148","pmid":null,"published_date":"2021 Apr 12"},"status":"released","submitted_date":"2019-06-28","released_date":"2020-10-28"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDF48.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF48.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF48_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF48_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF48_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF48_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF48.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Myotilin Ig1Ig2 (250-498)","short_name":"Ig1Ig2 (250-498)","sequence":"GPAMPRFIQVPENMSIDEGRFCRMDFKVSGLPAPDVSWYLNGRTVQSDDLHKMIVSEKGLHSLIFEVVRASDAGAYACVAKNRAGEATFTVQLDVLAKEHKRAPMFIYKPQSKKVLEGDSVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYQDNTGRVTLLIKDVNKKDAGWYTVSAVNEAGVTTCNTRLDVTARPNQTLPAPKQLRVRPTFSKYLALNGKGLNVKQAFNPEGEFQRLAAQSGLYESEEL","organism":"Homo sapiens","uniprot_code":"Q9UBF9","uniprot_range_first":250,"uniprot_range_last":498,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MFNYERPKHFIQSQNPCGSRLQPPGPETSSFSSQTKQSSIIIQPRQCTEQRFSASSTLSS\nHITMSSSAFPASPKQHAGSNPGQRVTTTYNQSPASFLSSILPSQPDYNSSKIPSAMDSNY\nQQSSAGQPINAKPSQTANAKPIPRTPDHEIQGSKEALIQDLERKLKCKDTLLHNGNQRLT\nYEEKMARRLLGPQNAAAVFQAQDDSGAQDSQQHNSEHARLQVPTSQVRSRSTSRGDVNDQ\nDAIQEKFYPPRFIQVPENMSIDEGRFCRMDFKVSGLPAPDVSWYLNGRTVQSDDLHKMIV\nSEKGLHSLIFEVVRASDAGAYACVAKNRAGEATFTVQLDVLAKEHKRAPMFIYKPQSKKV\nLEGDSVKLECQISAIPPPKLFWKRNNEMVQFNTDRISLYQDNTGRVTLLIKDVNKKDAGW\nYTVSAVNEAGVTTCNTRLDVTARPNQTLPAPKQLRVRPTFSKYLALNGKGLNVKQAFNPE\nGEFQRLAAQSGLYESEEL","mw":28.31,"total_mw":28.31,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Myotilin immunoglobulin domains Ig1Ig2 (250-498)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Melissa ","contributor_surname":"Graewert","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2019-06-27","storage_temperature":null,"cell_temperature":20.0,"exposure_time":15.0,"number_of_frames":1,"wavelength":0.099,"sample_detector_distance":2.867,"concentration_min":null,"concentration_max":15.2,"sample_volume":null,"flow_rate":null,"s_min":0.032,"s_max":4.494,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of Myotilin Ig1Ig2 (250-498) in 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 15.20 mg/ml was measured at 20°C. One 15 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":14.9,"experimental_mw_error":null,"guinier_i0_mw":14.87,"guinier_i0_mw_error":null,"porod_mw":23.0,"porod_mw_error":null,"pddf_i0":15.06,"pddf_i0_error":null,"guinier_i0":14.87,"guinier_i0_error":0.01,"pddf_rg":3.418,"pddf_rg_error":null,"guinier_rg":3.22,"guinier_rg_error":0.02,"pddf_dmax":14.0,"pddf_dmax_error":null,"porod_volume":37.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":29,"guinier_point_last":73,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF48_datloglog_img.png","symmetry":null,"last_modified":"2020-10-28T14:25:06.632032+01:00","bragg_peak":[]}