{"code":"SASDF49","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.","publication":{"title":"Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.","author_list":"Yadav DK, Zigáčková D, Zlobina M, Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkz1163","pmid":"31875226","published_date":"2019 Dec 25"},"status":"released","submitted_date":"2019-07-08","released_date":"2019-11-29"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDF49.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF49.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF49_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF49_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF49_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF49_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF49.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4","short_name":"STAU1-RBD34","sequence":"GSHMKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKNAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKEKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFK","organism":"Homo sapiens","uniprot_code":"O95793","uniprot_range_first":183,"uniprot_range_last":355,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSQVQVQVQNPSAALSGSQILNKNQSLLSQPLMSIPSTTSSLPSENAGRPIQNSALPSAS\nITSTSAAAESITPTVELNALCMKLGKKPMYKPVDPYSRMQSTYNYNMRGGAYPPRYFYPF\nPVPPLLYQVELSVGGQQFNGKGKTRQAAKHDAAAKALRILQNEPLPERLEVNGRESEEEN\nLNKSEISQVFEIALKRNLPVNFEVARESGPPHMKNFVTKVSVGEFVGEGEGKSKKISKKN\nAAIAVLEELKKLPPLPAVERVKPRIKKKTKPIVKPQTSPEYGQGINPISRLAQIQQAKKE\nKEPEYTLLTERGLPRRREFVMQVKVGNHTAEGTGTNKKVAKRNAAENMLEILGFKVPQAQ\nPTKPALKSEEKTPIKKPGDGRKVTFFEPGSGDENGTSNKEDEFRMPYLSHQQLPAGILPM\nVPEVAQAVGVSQGHHTKDFTRAAPNPAKATVTAMIARELLYGGTSPTAETILKNNISSGH\nVPHGPLTRPSEQLDYLSRVQGFQVEYKDFPKNNKNEFVSLINCSSQPPLISHGIGKDVES\nCHDMAALNILKLLSELDQQSTEMPRTGNGPMSVCGRC","mw":19.733,"total_mw":19.733,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"ADP-ribosylation factor1 - long","short_name":"ARF1 RNA - long","sequence":"GGCGAGUGCCAGAAGCUGUCUCCUUCGGGAGGCAGUUUCUGGUACUCGCC","organism":"Homo sapiens","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"RNA","uniprot_sequence":null,"mw":16.137,"total_mw":16.137,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.8,"deuteration":null},"purity_method":null,"name":"Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":null,"full_name":"CEITEC - Central European Institute of Technology, Masaryk University","webpage":"https://www.ceitec.eu/"}],"contributor_name":"Tomas","contributor_surname":"Klumpler","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2018-09-20","storage_temperature":null,"cell_temperature":15.0,"exposure_time":3.0,"number_of_frames":1,"wavelength":0.1025,"sample_detector_distance":null,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.206,"s_max":3.001,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long in 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol, pH 6.8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.1025 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 15°C. One 3 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":34.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":33.0,"porod_mw_error":null,"pddf_i0":0.009973,"pddf_i0_error":null,"guinier_i0":0.01024,"guinier_i0_error":7.8e-05,"pddf_rg":2.46,"pddf_rg_error":null,"guinier_rg":2.536,"guinier_rg_error":0.063,"pddf_dmax":6.97,"pddf_dmax_error":null,"porod_volume":53.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":28,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF49_datloglog_img.png","symmetry":null,"last_modified":"2020-02-12T18:29:36.587247+01:00","bragg_peak":[]}