{"code":"SASDF52","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless.","publication":{"title":"Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless.","author_list":"Ankush Jagtap PK, Müller M, Masiewicz P, von Bülow S, Hollmann NM, Chen PC, Simon B, Thomae AW, Becker PB, Hennig J","journal":"Nucleic Acids Res","doi":"10.1093/nar/gkz125","pmid":"30805612","published_date":"2019 May 7"},"status":"released","submitted_date":"2019-01-28","released_date":"2019-02-21"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDF52.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF52.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF52_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF52_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF52_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF52_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF52.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Dosage compensation regulator","short_name":"MLE","sequence":"> dsRBD12_single | chain A | 259 aa\r\nGAMDIKSFLYQFCAKSQIEPKFDIRQTGPKNRQRFLCEVRVEPNTYIGVGNSTNKKDAEK\r\nNACRDFVNYLVRVGKLNTNDVPADAGASGGGPRTGLEGAGMAGGSGQQKRVFDGQSGPQD\r\nLGEAYRPLNHDGGDGGNRYSVIDRIQEQRDMNEAEAFDVNAAIHGNWTIENAKERLNIYK\r\nQTNNIRDDYKYTPVGPEHARSFLAELSIYVPALNRTVTARESGSNKKSASKSCALSLVRQ\r\nLFHLNVIEPFSGTLKKKKD","organism":"Drosophila melanogaster","uniprot_code":"P24785","uniprot_range_first":1,"uniprot_range_last":257,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MDIKSFLYQFCAKSQIEPKFDIRQTGPKNRQRFLCEVRVEPNTYIGVGNSTNKKDAEKNA\nCRDFVNYLVRVGKLNTNDVPADAGASGGGPRTGLEGAGMAGGSGQQKRVFDGQSGPQDLG\nEAYRPLNHDGGDGGNRYSVIDRIQEQRDMNEAEAFDVNAAIHGNWTIENAKERLNIYKQT\nNNIRDDYKYTPVGPEHARSFLAELSIYVPALNRTVTARESGSNKKSASKSCALSLVRQLF\nHLNVIEPFSGTLKKKKDEQLKPYPVKLSPNLINKIDEVIKGLDLPVVNPRNIKIELDGPP\nIPLIVNLSRIDSSQQDGEKRQESSVIPWAPPQANWNTWHACNIDEGELATTSIDDLSMDY\nERSLRDRRQNDNEYRQFLEFREKLPIAAMRSEILTAINDNPVVIIRGNTGCGKTTQIAQY\nILDDYICSGQGGYANIYVTQPRRISAISVAERVARERCEQLGDTVGYSVRFESVFPRPYG\nAILFCTVGVLLRKLEAGLRGVSHIIVDEIHERDVNSDFLLVILRDMVDTYPDLHVILMSA\nTIDTTKFSKYFGICPVLEVPGRAFPVQQFFLEDIIQMTDFVPSAESRRKRKEVEDEEQLL\nSEDKDEAEINYNKVCEDKYSQKTRNAMAMLSESDVSFELLEALLMHIKSKNIPGAILVFL\nPGWNLIFALMKFLQNTNIFGDTSQYQILPCHSQIPRDEQRKVFEPVPEGVTKIILSTNIA\nETSITIDDIVFVIDICKARMKLFTSHNNLTSYATVWASKTNLEQRKGRAGRVRPGFCFTL\nCSRARFQALEDNLTPEMFRTPLHEMALTIKLLRLGSIHHFLSKALEPPPVDAVIEAEVLL\nREMRCLDANDELTPLGRLLARLPIEPRLGKMMVLGAVFGCADLMAIMASYSSTFSEVFSL\nDIGQRRLANHQKALSGTKCSDHVAMIVASQMWRREKQRGEHMEARFCDWKGLQMSTMNVI\nWDAKQQLLDLLQQAGFPEECMISHEVDERIDGDDPVLDVSLALLCLGLYPNICVHKEKRK\nVLTTESKAALLHKTSVNCSNLAVTFPYPFFVFGEKIRTRAVSCKQLSMVSPLQVILFGSR\nKIDLAANNIVRVDNWLNFDIEPELAAKIGALKPALEDLITVACDNPSDILRLEEPYAQLV\nKVVKDLCVKSAGDFGLQRESGILPHQSRQFSDGGGPPKRGRFETGRFTNSSFGRRGNGRT\nFGGGYGNNGGGYGNNGGGYGNIGGGYGNNAGGYGNNGGYGNNGGGYRNNGGGYGNNGGGY\nGNKRGGFGDSFESNRGSGGGFRNGDQGGRWGNF","mw":28.549,"total_mw":28.549,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM NaPO4, 200 mM NaCl, 1 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.5,"deuteration":null},"purity_method":null,"name":"dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL","address":"Heidelberg, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Heidelberg","webpage":"https://www.embl.de/"}],"contributor_name":"Po-chia","contributor_surname":"Chen","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-11-29","storage_temperature":null,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":10,"wavelength":0.09919,"sample_detector_distance":2.867,"concentration_min":5.0,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.035,"s_max":4.944,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDF52_fit1_model1_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDF52_fit1_model1.pdb","model_mw":29.13,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":297},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDF52_fit1_model2_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDF52_fit1_model2.pdb","model_mw":29.13,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":297},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDF52_fit1_model3_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDF52_fit1_model3.pdb","model_mw":29.13,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":297},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDF52_fit1_model4_img.png","software":"EOM/RANCH","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDF52_fit1_model4.pdb","model_mw":29.13,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":297}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDF52_fit1_fixed_fit_img.png","software":"EOM/GAJOE","chi_square_value":2.089,"p_value":0.0,"fit_residual_plot":"SASDF52_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDF52_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/logFile_001_1_uBn8rj9.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"Construct contains an additional two amino acids (Gly-Ala) at the N-terminus as a remaining product the TEV-protease cleavage.\r\nThe data displayed in this entry consists of merged data from three replicates of equal sample concentration (5.0 mg/ml). Thus, an effective total of 30 frames x 1s exposures is collated for this curve. The data were merged using Primus and datmerge of the ATSAS 2.8.4-1 package maintained by SBGrid.","experiment_description":"Synchrotron SAXS data from solutions of the dsRBD1 and dsRBD2 domains of Drosophila helicase dosage compensation regulator, MLE, in 20 mM NaPO4, 200 mM NaCl, 1 mM DTT, pH 6.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). A solute concentration of 5 mg/ml was measured at 20°C. 10 successive 1 second frames were collected per sample (three replicates total). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":14.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":14.0,"porod_mw_error":null,"pddf_i0":66.44,"pddf_i0_error":null,"guinier_i0":66.26,"guinier_i0_error":0.13,"pddf_rg":3.337,"pddf_rg_error":null,"guinier_rg":3.23,"guinier_rg_error":0.13,"pddf_dmax":12.5,"pddf_dmax_error":null,"porod_volume":22.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":6,"guinier_point_last":60,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF52_datloglog_img.png","symmetry":null,"last_modified":"2020-08-28T15:27:32.015613+02:00","bragg_peak":[]}