{"code":"SASDF68","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase.","publication":{"title":"Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase.","author_list":"Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L","journal":"J Am Chem Soc","doi":"10.1021/jacs.9b07837","pmid":"31550880","published_date":"2019 Sep 24"},"status":"released","submitted_date":"2019-06-22","released_date":"2019-09-30"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF68.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF68_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF68_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF68_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF68.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"DNA-directed RNA polymerase subunit delta","short_name":"RpoE","sequence":"MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK","organism":"Bacillus subtilis","uniprot_code":"P12464","uniprot_range_first":1,"uniprot_range_last":173,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYT\nDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDE\nDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK","mw":20.399,"total_mw":20.399,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.6,"deuteration":null},"purity_method":null,"name":"Delta subunit of RNA polymerase, RNAP (B. subtilis), 200 mM NaCl","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"MUNI","address":"Brno, Czech Republic","full_name":"Masaryk University;   \tResearch group Structure and Dynamics of Proteins.","webpage":"https://www.muni.cz/"}],"contributor_name":"Vojtěch","contributor_surname":"Kubáň","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-10-03","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":null,"concentration_max":3.6,"sample_volume":null,"flow_rate":null,"s_min":0.013,"s_max":3.79,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":"No concentration effect was observed in higher salt concentration buffers.","experiment_description":"Synchrotron SAXS data from solutions of RNAP in 20 mM phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH 6.6 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.60 mg/ml was measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":33.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":35.0,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":7618.0,"guinier_i0_error":27.0,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":3.93,"guinier_rg_error":0.35,"pddf_dmax":20.0,"pddf_dmax_error":null,"porod_volume":56.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":39,"guinier_point_last":150,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF68_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}