{"code":"SASDF73","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms","publication":{"title":"Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasms","author_list":"Duarte D, Lamontanara A, La Sala G, Jeong S, Sohn Y, Panjkovich A, Georgeon S, Kükenshöner T, Marcaida M, Pojer F, De Vivo M, Svergun D, Kim H, Dal Peraro M, Hantschel O","journal":"Nature Communications","doi":"10.1038/s41467-020-16128-5","pmid":null,"published_date":"2020 May 8"},"status":"released","submitted_date":"2019-02-20","released_date":"2020-01-08"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDF73.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF73.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF73_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF73_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF73_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF73_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF73.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Bruton's tyrosine kinase - 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Solute concentrations ranging between 0.78 and 4.32 mg/ml were measured at 10°C. 10 successive 0.5 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The raw curve measured from highest concentration was used for further analysis. The final scattering curve was analysed using PRIMUS software (ATSAS). The model depicts the DAMMIN ab initio reconstruction superimposed with the crystal structure 4XI2 (PDB). Ensemble optimisation method (EOM) was performed using three domains structure (1QLY, 2GE9 and 1K2P, PDB) to assess construct’s flexibility.","tags":[],"intensity_unit":null,"experimental_mw":45.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":45.0,"porod_mw_error":null,"pddf_i0":44.18,"pddf_i0_error":null,"guinier_i0":44.28,"guinier_i0_error":0.07,"pddf_rg":2.62,"pddf_rg_error":null,"guinier_rg":2.62,"guinier_rg_error":0.01,"pddf_dmax":8.3,"pddf_dmax_error":null,"porod_volume":72.26,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":18,"guinier_point_last":98,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF73_datloglog_img.png","symmetry":null,"last_modified":"2020-08-28T15:27:50.651789+02:00","bragg_peak":[]}