{"code":"SASDF93","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"The archaeal LDH-like malate dehydrogenase from Ignicoccus islandicus displays dual substrate recognition, hidden allostery and a non-canonical tetrameric oligomeric organization","publication":{"title":"The archaeal LDH-like malate dehydrogenase from Ignicoccus islandicus displays dual substrate recognition, hidden allostery and a non-canonical tetrameric oligomeric organization","author_list":"Roche J, Girard E, Mas C, Madern D","journal":"Journal of Structural Biology","doi":"/10.1016/j.jsb.2019.07.006","pmid":null,"published_date":"2019 Jul"},"status":"released","submitted_date":"2019-02-20","released_date":"2019-07-11"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDF93.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF93.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF93_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF93_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF93_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF93_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF93.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Malate dehydrogenase","short_name":null,"sequence":"MARIPYKVAVIGTGRVGATFAYTMAVVPGIARMTLVDVVPGLAKGVMEDIKHAAAVFRRSITVEAFEDVSKVENADAIVITAGKPRKADMSRRDLANVNAQIIRDIGDKLRDRNPGALYVVVTNPVDVMTMVLDDVIGSKGTVIGTGTSLDTFRFRAAVSELLNVPIVAVDGYVVGEHGEEAFVAWSTVTIKGIHIDQYIKERNINISREQIEKYVKDVAASIIASQGATIWGPAATFQEIVVSHLANESKIIPISLPQNIEGVGRVAVSVPTIISGRLKPLVQLLNEEEQERLKRAAKAIRNVYESILT","organism":"Ignicoccus islandicus DSM 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dehydrogenase","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"IBS","address":"Grenoble, France","full_name":"Institut de Biologie Structurale","webpage":""}],"contributor_name":"Jennifer","contributor_surname":"Roche","orcid":"https://orcid.org/0000-0002-6234-1340"}],"concentration_method":null,"concentration_unit":null,"date":"2018-09-05","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":1.0,"number_of_frames":10,"wavelength":0.125,"sample_detector_distance":2.85,"concentration_min":0.39,"concentration_max":9.31,"sample_volume":null,"flow_rate":null,"s_min":0.045,"s_max":4.946,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDF93_fit1_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"6qss","ext_code":"6QSS","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDF93_fit1_model1.pdb","model_mw":135.2,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":419}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/refine00_fit_img.png","software":"CRYSOL","chi_square_value":1.678,"p_value":0.0,"fit_residual_plot":"refine00_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDF93_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/refine00.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of I. islandicus malate dehydrogenase in 50 mM Tris-HCl 50 mM NaCl, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.125 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 9.3 mg/ml were measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"arbitrary","experimental_mw":118.75,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":124.0,"porod_mw_error":null,"pddf_i0":107.7,"pddf_i0_error":null,"guinier_i0":109.12,"guinier_i0_error":0.61,"pddf_rg":3.163,"pddf_rg_error":null,"guinier_rg":3.26,"guinier_rg_error":0.03,"pddf_dmax":8.96,"pddf_dmax_error":null,"porod_volume":198.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":17,"guinier_point_last":75,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF93_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}