{"code":"SASDF95","status":"Published","type_of_curve":"Merged","angular_unit":"1/A","project":{"title":"Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa","publication":{"title":"Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa","author_list":"Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H","journal":"Frontiers in Microbiology","doi":"10.3389/fmicb.2019.03158","pmid":null,"published_date":"2020 Jan 22"},"status":"released","submitted_date":"2019-04-04","released_date":"2020-01-24"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDF95.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF95.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF95_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF95_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDF95_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF95_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF95.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Shanghai Synchrotron Radiation Facility (SSRF)","city":"Shanghai","country":"China","beamline_name":"BL19U2","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Uncharacterized protein","short_name":null,"sequence":"MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLG\r\nRYFDTSAQFWMNLQSEYSLATAYAANGKQIEHEIEPLLAHG","organism":"Pseudomonas aeruginosa","uniprot_code":"Q9HVC1","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLG\nRYFDTSAQFWMNLQSEYSLATAYAANGKQIEHEIEPLLAHG","mw":11.248,"total_mw":22.496,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"DNA Duplex","short_name":null,"sequence":"TGAAGTTAACGCTTAACGTTAAGGGTTAATGA","organism":null,"uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"dimer","molecular_type":"DNA","uniprot_sequence":null,"mw":10.006,"total_mw":20.012,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Pseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA-DNA complex","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Shanghai, China","full_name":"National Center for Protein Science Shanghai","webpage":"http://www.sibcb-ncpss.org"}],"contributor_name":"Guangfeng","contributor_surname":"Liu","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-09-19","storage_temperature":10.0,"cell_temperature":23.0,"exposure_time":1.0,"number_of_frames":20,"wavelength":0.103,"sample_detector_distance":2.7,"concentration_min":1.0,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.07,"s_max":3.348,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDF95_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDF95_fit1_model1.pdb","model_mw":null,"bead_radius":3.0,"log":"https://www.sasbdb.org/media/log_files/AO28.log","symmetry":"p1","comment":null,"user":434}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDF95_fit1_fixed_fit_img.png","software":"DAMMIF","chi_square_value":1.053,"p_value":0.01926,"fit_residual_plot":"SASDF95_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDF95_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/AO28.log","software_version":"2.8.4","description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDF95_fit2_model1_img.png","software":"PDB (Protein Data Bank)","pdb_link":[{"pdb_code":"6jpi","ext_code":"6JPI","ext_type":"pdb","difference_with_model":"identical"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDF95_fit2_model1.pdb","model_mw":58.78,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/4D.log","symmetry":null,"comment":null,"user":434}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDF95_fit2_fixed_fit_img.png","software":"CRYSOL","chi_square_value":6.229,"p_value":0.0,"fit_residual_plot":"SASDF95_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDF95_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/4D.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the PaHigA-DNA complex in 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, 1 mM PMSF, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 23°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":"arbitrary","experimental_mw":73.8,"experimental_mw_error":null,"guinier_i0_mw":73.8,"guinier_i0_mw_error":1.2,"porod_mw":null,"porod_mw_error":null,"pddf_i0":80.36,"pddf_i0_error":null,"guinier_i0":80.36,"guinier_i0_error":0.17,"pddf_rg":2.98,"pddf_rg_error":null,"guinier_rg":2.935,"guinier_rg_error":0.008,"pddf_dmax":9.83,"pddf_dmax_error":null,"porod_volume":81.1,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":34,"guinier_point_last":95,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF95_datloglog_img.png","symmetry":null,"last_modified":"2020-01-24T10:37:27.754832+01:00","bragg_peak":[]}