{"code":"SASDF98","status":"Published","type_of_curve":"Extrapolated to infinite dilution","angular_unit":"1/nm","project":{"title":"Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase.","publication":{"title":"Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase.","author_list":"Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L","journal":"J Am Chem Soc","doi":"10.1021/jacs.9b07837","pmid":"31550880","published_date":"2019 Sep 24"},"status":"released","submitted_date":"2019-06-22","released_date":"2019-09-30"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDF98.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF98_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF98_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDF98_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDF98.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"DNA-directed RNA polymerase subunit delta - mutant","short_name":"rpoE KE mutant","sequence":"MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYTDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVEAEEEEAEEAVEEDLDLDEFEEIDEDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK","organism":"Bacillus subtilis","uniprot_code":"P12464","uniprot_range_first":1,"uniprot_range_last":173,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MGIKQYSQEELKEMALVEIAHELFEEHKKPVPFQELLNEIASLLGVKKEELGDRIAQFYT\nDLNIDGRFLALSDQTWGLRSWYPYDQLDEETQPTVKAKKKKAKKAVEEDLDLDEFEEIDE\nDDLDLDEVEEELDLEADDFDEEDLDEDDDDLEIEEDIIDEDDEDYDDEEEEIK","mw":20.405,"total_mw":20.405,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.6,"deuteration":null},"purity_method":null,"name":"Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"MUNI","address":"Brno, Czech Republic","full_name":"Masaryk University;   \tResearch group Structure and Dynamics of Proteins.","webpage":"https://www.muni.cz/"}],"contributor_name":"Vojtěch","contributor_surname":"Kubáň","orcid":null}],"concentration_method":null,"concentration_unit":null,"date":"2016-10-03","storage_temperature":20.0,"cell_temperature":20.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":3.0,"concentration_min":0.8,"concentration_max":2.6,"sample_volume":null,"flow_rate":null,"s_min":0.013,"s_max":3.792,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":"There is a concentration effect in the concentration series for the sample in 10 mM salt buffer, so we use extrapolation to infinite dilution.","experiment_description":"Synchrotron SAXS data from solutions of Lys-Glu mutant RNAP in 20 mM phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH 6.6 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.8 and 2.6 mg/ml were measured at 20°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.","tags":[],"intensity_unit":"arbitrary","experimental_mw":23.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":36.0,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":4905.0,"guinier_i0_error":49.0,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":4.29,"guinier_rg_error":0.6,"pddf_dmax":19.5,"pddf_dmax_error":null,"porod_volume":58.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":21,"guinier_point_last":139,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDF98_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}