{"code":"SASDFA5","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Structure, Function, and Dynamics of the Gα Binding Domain of Ric-8A.","publication":{"title":"Structure, Function, and Dynamics of the Gα Binding Domain of Ric-8A.","author_list":"Zeng B, Mou TC, Doukov TI, Steiner A, Yu W, Papasergi-Scott M, Tall GG, Hagn F, Sprang SR","journal":"Structure","doi":"10.1016/j.str.2019.04.013","pmid":"31155309","published_date":"2019 May 9"},"status":"released","submitted_date":"2019-04-08","released_date":"2019-06-05"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFA5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFA5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFA5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFA5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFA5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFA5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFA5.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Rayonix MX225-HE","resolution":null},"name":"Stanford Synchrotron Radiation Lightsource (SSRL)","city":"Menlo Park, CA","country":"USA","beamline_name":"BL4-2","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Resistance to inhibitors of cholinesterase 8 homolog A","short_name":"Ric-8A","sequence":"GMEPRAVADALETGEEDAVTEALRSFNREHSQSFTFDDAQQEDRKRLAKLLVSVLEQGLSPKHRVTWLQTI\r\nRILSRDRSCLDSFASRQSLHALACYADIAISEEPIPQPPDMDVLLESLKCLCNLVLSSPTAQMLAAEARL\r\nVVRLAERVGLYRKRSYPHEVQFFDLRLLFLLTALRTDVRQQLFQELHGVRLLTDALELTLGVAPKENPLV\r\nILPAQETERAMEILKVLFNITFDSVKREVDEEDAALYRYLGTLLRHCVMADAAGDRTEEFHGHTVNLLGN\r\nLPLKCLDVLLALELHEGSLEFMGVNMDVINALLAFLEKRLHQTHRLKECVAPVLSVLTECARMHRPARKF\r\nLKAQVLPPLRDVRTRPEVGDLLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLA\r\nARGLMAGGRPEGQYSEDEDTDTEEYREAKASI","organism":"Rattus norvegicus","uniprot_code":"B1H241","uniprot_range_first":1,"uniprot_range_last":452,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MEPRAVADALETGEEDAVTEALRSFNREHSQSFTFDDAQQEDRKRLAKLLVSVLEQGLSP\nKHRVTWLQTIRILSRDRSCLDSFASRQSLHALACYADIAISEEPIPQPPDMDVLLESLKC\nLCNLVLSSPTAQMLAAEARLVVRLAERVGLYRKRSYPHEVQFFDLRLLFLLTALRTDVRQ\nQLFQELHGVRLLTDALELTLGVAPKENPLVILPAQETERAMEILKVLFNITYDSVKREVD\nEEDAALYRYLGTLLRHCVMADAAGDRTEEFHGHTVNLLGNLPLKCLDVLLALELHEGSLE\nFMGVNMDVINALLAFLEKRLHQTHRLKECVAPVLSVLTECARMHRPARKFLKAQVLPPLR\nDVRTRPEVGDLLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLA\nARGLMAGGRPEGQYSEDEDTDTEEYREAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAM\nKLVNMFDKLSRHRLIQPMGMSPRGHLTSLQDAMCETMEGQLSSDPDSDPD","mw":51.09,"total_mw":51.09,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM HEPES, 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Unposphorylated Resistance to inhibitors of cholinesterase 8 homolog A, Ric-8A, amino acids 1-452 (Rattus norvegicus)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Montana, USA","full_name":"University of Montana","webpage":"https://www.umt.edu/"}],"contributor_name":"Tung-Chung","contributor_surname":"Mou","orcid":"https://orcid.org/0000-0001-6554-1519"}],"concentration_method":null,"concentration_unit":null,"date":"2018-04-24","storage_temperature":4.0,"cell_temperature":23.0,"exposure_time":1.0,"number_of_frames":5,"wavelength":0.1127,"sample_detector_distance":1.7,"concentration_min":null,"concentration_max":1.0,"sample_volume":null,"flow_rate":null,"s_min":0.065,"s_max":3.205,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"CAUTION! The data used to calculate p(r) profile are different to primary SAXS data.","experiment_description":"Synchrotron SAXS data from solutions of unposphorylated Ric-8A (amino acids 1-452) in 25mM HEPES, 150mM NaCl, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 1.7 m and at a wavelength of λ = 0.1127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 25 °C using the following parameters: Column: Superdex 200 Increase 3.2/300 analytical SEC column; Flow rate: 0.05 mL/min; Sample injection concentration: 1.0 mg/mL; Injection volume: 100 μL. The data were collected through the SEC peak of the sample as a series of 5 x 1 second exposures. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":51.2,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":44.0,"porod_mw_error":null,"pddf_i0":106.8,"pddf_i0_error":null,"guinier_i0":84.58,"guinier_i0_error":0.081,"pddf_rg":2.99,"pddf_rg_error":null,"guinier_rg":2.963,"guinier_rg_error":0.02,"pddf_dmax":10.62,"pddf_dmax_error":null,"porod_volume":70.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":15,"guinier_point_last":69,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFA5_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}