{"code":"SASDFC5","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/nm","project":{"title":"Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.","publication":{"title":"Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation.","author_list":"Voss M, Toelzer C, Bhandari DD, Parker JE, Niefind K","journal":"J Struct Biol","doi":"10.1016/j.jsb.2019.09.007","pmid":"31550533","published_date":"2019 Sep 21"},"status":"released","submitted_date":"2019-04-08","released_date":"2019-11-14"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFC5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFC5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFC5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFC5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFC5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFC5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFC5.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Enhanced disease susceptibility","short_name":"EDS1","sequence":">sp|Q9SU72|EDS1C_ARATH Protein EDS1 OS=Arabidopsis thaliana OX=3702 GN=EDS1 PE=1 SV=1\r\nMAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSS\r\nFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFT\r\nGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFV\r\nNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVA\r\nNQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQA\r\nSDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQA\r\nALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFK\r\nANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNE\r\nDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNS\r\nGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWI\r\nTLPKNHKSHSPLRDYMMDEITDT","organism":"Arabidopsis thaliana","uniprot_code":"Q9SU72","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MAFEALTGINGDLITRSWSASKQAYLTERYHKEEAGAVVIFAFQPSFSEKDFFDPDNKSS\nFGEIKLNRVQFPCMRKIGKGDVATVNEAFLKNLEAIIDPRTSFQASVEMAVRSRKQIVFT\nGHSSGGATAILATVWYLEKYFIRNPNVYLEPRCVTFGAPLVGDSIFSHALGREKWSRFFV\nNFVSRFDIVPRIMLARKASVEETLPHVLAQLDPRKSSVQESEQRITEFYTRVMRDTSTVA\nNQAVCELTGSAEAFLETLSSFLELSPYRPAGTFVFSTEKRLVAVNNSDAILQMLFYTSQA\nSDEQEWSLIPFRSIRDHHSYEELVQSMGKKLFNHLDGENSIESTLNDLGVSTRGRQYVQA\nALEEEKKRVENQKKIIQVIEQERFLKKLAWIEDEYKPKCQAHKNGYYDSFKVSNEENDFK\nANVKRAELAGVFDEVLGLMKKCQLPDEFEGDIDWIKLATRYRRLVEPLDIANYHRHLKNE\nDTGPYMKRGRPTRYIYAQRGYEHYILKPNGMIAEDVFWNKVNGLNLGLQLEEIQETLKNS\nGSECGSCFWAEVEELKGKPYEEVEVRVKTLEGMLGEWITDGEVDDKEIFLEGSTFRKWWI\nTLPKNHKSHSPLRDYMMDEITDT","mw":71.689,"total_mw":71.689,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Enhanced disease susceptibility 1 (EDS1)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Germany","full_name":"University of Cologne","webpage":"http://uni-koeln.de/"}],"contributor_name":"Martin","contributor_surname":"Voss","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2015-05-19","storage_temperature":4.0,"cell_temperature":4.0,"exposure_time":2.0,"number_of_frames":1495,"wavelength":0.099,"sample_detector_distance":2.867,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.036,"s_max":4.939,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFC5_fit1_model1_img.png","software":"DAMMIN","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFC5_fit1_model1.pdb","model_mw":57.855,"bead_radius":2.6,"log":null,"symmetry":null,"comment":null,"user":414}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/EDS1komp_fixed_fit_img.png","software":"DAMMIN","chi_square_value":0.824,"p_value":0.059642,"fit_residual_plot":"EDS1komp_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFC5_fit1.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The data were re-binned to calculate the p(r) profile and corresponding model fit.","experiment_description":"Synchrotron SAXS data from solutions of enhanced disease susceptibility 1 (EDS1) in 50 mM NaCl, 50 mM HEPES, 1% glyercol, 1 mM DTT, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 4 °C using the following parameters: Column: Superdex75 10/300GL; Flow rate: 0.4  mL/min; Sample injection concentration: 5 mg/mL; Injection volume: 500 μL. 1495 successive 2 second frames were collected through the SEC-elution and processed using CHROMIXS. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted from the sample frames. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":72.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":58.0,"porod_mw_error":null,"pddf_i0":152.6,"pddf_i0_error":null,"guinier_i0":151.87,"guinier_i0_error":0.47,"pddf_rg":3.08,"pddf_rg_error":null,"guinier_rg":3.09,"guinier_rg_error":0.02,"pddf_dmax":10.53,"pddf_dmax_error":null,"porod_volume":92.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":23,"guinier_point_last":80,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFC5_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}