{"code":"SASDFD4","status":"Published","type_of_curve":"Merged","angular_unit":"1/nm","project":{"title":"Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1.","publication":{"title":"Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1.","author_list":"Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI","journal":"J Biol Chem","doi":"10.1074/jbc.RA119.008661","pmid":"31350339","published_date":"2019 Sep 13"},"status":"released","submitted_date":"2019-03-20","released_date":"2019-07-26"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFD4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFD4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFD4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFD4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFD4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFD4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFD4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Condensin complex subunit 3-like protein","short_name":"Ycg1","sequence":">tr|G0SHK2|G0SHK2_CHATD Condensin complex subunit 3-like protein OS=Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) 24-1006\r\nPDNALRTQVTTVFREAQRTTASHRKLVVTLRKIQEACCYEPATGQKQSGAAAALSEFDED\r\nDFNTEFVRCALRILPVKKSEGVGEKTVRFVSLFLRHAIEKDNELLGDTQMDEDDADNSTM\r\nPETPSTRLTSHLMETILPMLMAKDKFVRYRAAQLLAHIVNSLDAIDDDLYQKLRAGLLRR\r\nIHDKEPMVRAQAVLGLGRLAGNALDEEPGAGDSDLDGDDDGPGGASGVSLMDKLMDVMKN\r\nDPSADVRRTLLVNLPITPKTLPSLLERARDQDPATRRAVYSRLLPALGDFRHLSLSMREK\r\nLLRWGLRDRDENVRKAAARLFRERWIEDCAGTPRPEPGQPIELSPPNMDALLELLERIDV\r\nVNSGGENGIALEAMRGFWEGRPDYRDAMVFDDHFFETLSAESVFALRTFNDFCRNEAGGK\r\nYEALIEEKLPEVTKLAFYLERYIKVLIDAIKRAEEQGEEEEEDTAEQEFIVEQLLHIALT\r\nLDYADEVGRRKMFALLRQTLSIPELPEECTKLTVMVLRDLCAPDAAGEKEFTSVVLEAVA\r\nDVHDTIVDEDDDDANVPAEDDESFVSARSEVSRESTPAPGGGEGGGSNSRKRGRSPTPKM\r\nTEEEAAAKAIREIMINMKCLHIVQCMLQNVQGQLQSNDNLISMLNNLVVPAVRSHEAPVR\r\nERGLVCLGLCALLDRSLAEENLTLFIHFFAKGHTALQITALHILTDILVVHGAQLLSANP\r\nTLLKVYVKALRSGAKHPELQAVAVVAASKLLLGRVVSDRDVAAELLKTLVVAYFEPASAG\r\nNQSVRQALNYFLPVFCYSRAENQDLMRRVALDALHTLYNVREGFDDDDADIDDEMVSLAT\r\nIGACLVDWTDPRKVYTPDAGGLQAEKHVNADVHLDFAKAILERLEGNVPREEKKVLASLL\r\nSKLYISPASSPDKTRETYRVVSAAVDTNLLPDTTSRNALYKIHVSLGKIVNALDGAVPSV\r\nETASVAGSTGTGIGTTTTAATGR","organism":"Chaetomium thermophilum","uniprot_code":"G0SHK2","uniprot_range_first":24,"uniprot_range_last":1006,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MPPRTRSTRSSRSSSAIDIPEETPDNALRTQVTTVFREAQRTTASHRKLVVTLRKIQEAC\nCYEPATGQKQSGAAAALSEFDEDDFNTEFVRCALRILPVKKSEGVGEKTVRFVSLFLRHA\nIEKDNELLGDTQMDEDDADNSTMPETPSTRLTSHLMETILPMLMAKDKFVRYRAAQLLAH\nIVNSLDAIDDDLYQKLRAGLLRRIHDKEPMVRAQAVLGLGRLAGNALDEEPGAGDSDLDG\nDDDGPGGASGVSLMDKLMDVMKNDPSADVRRTLLVNLPITPKTLPSLLERARDQDPATRR\nAVYSRLLPALGDFRHLSLSMREKLLRWGLRDRDENVRKAAARLFRERWIEDCAGTPRPEP\nGQPIELSPPNMDALLELLERIDVVNSGGENGIALEAMRGFWEGRPDYRDAMVFDDHFFET\nLSAESVFALRTFNDFCRNEAGGKYEALIEEKLPEVTKLAFYLERYIKVLIDAIKRAEEQG\nEEEEEDTAEQEFIVEQLLHIALTLDYADEVGRRKMFALLRQTLSIPELPEECTKLTVMVL\nRDLCAPDAAGEKEFTSVVLEAVADVHDTIVDEDDDDANVPAEDDESFVSARSEVSRESTP\nAPGGGEGGGSNSRKRGRSPTPKMTEEEAAAKAIREIMINMKCLHIVQCMLQNVQGQLQSN\nDNLISMLNNLVVPAVRSHEAPVRERGLVCLGLCALLDRSLAEENLTLFIHFFAKGHTALQ\nITALHILTDILVVHGAQLLSANPTLLKVYVKALRSGAKHPELQAVAVVAASKLLLGRVVS\nDRDVAAELLKTLVVAYFEPASAGNQSVRQALNYFLPVFCYSRAENQDLMRRVALDALHTL\nYNVREGFDDDDADIDDEMVSLATIGACLVDWTDPRKVYTPDAGGLQAEKHVNADVHLDFA\nKAILERLEGNVPREEKKVLASLLSKLYISPASSPDKTRETYRVVSAAVDTNLLPDTTSRN\nALYKIHVSLGKIVNALDGAVPSVETASVAGSTGTGIGTTTTAATGRRVTRGSVSAGSGTG\nAGRRSISRASSVSTNADRPASASAAVPGSKAASTIEEEEEKEQEEEEEEEEKEEKEEEEE\nEEEEENVDEETQLAMQLQADTTAAQSREGSVQIKREEDDSESEASDATVVRGSVGTGRRV\nKQEEDDDF","mw":108.325,"total_mw":108.325,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Condensin complex subunit 2","short_name":"Brn1","sequence":">tr|G0SBJ6|G0SBJ6_CHATD Condensin complex subunit 2 OS=Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) 515-634\r\nKIHEDILSYFDQALQKNWTSAEHWRIRKIKDVNKPNEPTRQRKEKQPFEIDFVSPLDKAL\r\nAEIIYTKASSNATISMPKKDWKSKTKNLLPDDKHFSSKQLLSLFLKPKARMGRRRVLKTS","organism":"Chaetomium thermophilum","uniprot_code":"G0SBJ6","uniprot_range_first":515,"uniprot_range_last":634,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MPRVAPHRRSGSRVGSGSFLESPFKSPAKLPLADDAIEKAERAQGRKAAHEAQINQIRSA\nATSTPRKSSGAFNPDLDHGASPNSTPRTPHRRRSAVRETILLDDEEVVVGGRAVTPMKRV\nPILANFEEWMKMATDNKINAANSWNFALIDYFHDMSLLKEGDSVNFQKASCTLDGCVKIY\nTSRVDSVATETGKLLSGLADSRDSKKKDREDGGEDDDEEEEEVDEDGTVRKKPKKKTQRS\nSEATLAPSFASLQLKKLELEFAVDPFFKKASADFDEGGAKGLLLNHLMIDSQGRIVFDSS\nDDAEDVAEASAKPSREADDERPDSEDADADGDISMTDRDASAPGQVETQPEEEDEDVEID\nVAALGAKYFPDLSILDSLDVCPSLKTFDLGDPSGSLYIPFLKVPDDWRHDQEKEKTPGLG\nSLGNKSAMFIDDENPDGFDDDDGLGTFDMGADVGFGEGGEAWAKEAALEPHAMRVYDAGL\nGDGTEGDGEGDGADLMDAGTGEYVVAMSQPRSAEKIHEDILSYFDQALQKNWTSAEHWRI\nRKIKDVNKPNEPTRQRKEKQPFEIDFVSPLDKALAEIIYTKASSNATISMPKKDWKSKTK\nNLLPDDKHFSSKQLLSLFLKPKARMGRRRVLKTSRIGNIDSERHDDLPEGEVDEAFWATQ\nKAPLANGNDRPGRHPDDDDEPLPQGDYDANFFADDGLPFAGGMDDDDDMDRFSDAREHFS\nPEPGAGGPGMNSTAGLQDLATGIMGQNPGDGAFGTNLVTQGRRVRPEYVQYARVAKKVDV\nRRLKEEIWKGMGFDELTSSNSNDTSQLQTPARQAEEQRSPESADGPNGKDKDPTLRFTDV\nMNSLQRVYPKQVMDDISTSYCFICLLHLANEKGLVIEKTDTLDELYIRKDWSAVVGDE","mw":17.0,"total_mw":17.0,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris, 300 mM NaCl, 1mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Condensin complex subunit 3-like protein,Ycg1, bound to condensin complex subunit 2, Brn1 (Ycg1-Brn1)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Karen","contributor_surname":"Manalastas-Cantos","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2016-10-20","storage_temperature":13.0,"cell_temperature":10.0,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":4.0,"concentration_min":0.5,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.047,"s_max":2.511,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFD4_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"2.8.3","model_data":"https://www.sasbdb.org/media/pdb_file/SASDFD4_fit1_model1.pdb","model_mw":153.0,"bead_radius":1.7,"log":null,"symmetry":"P1","comment":null,"user":425}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/ycg1-kle-5_su5qzO1_fixed_fit_img.png","software":"DAMMIF","chi_square_value":0.684,"p_value":0.003314,"fit_residual_plot":"ycg1-kle-5_su5qzO1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFD4_fit1.fir","fit_log":null,"software_version":"2.8.3","description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFD4_fit2_model1_img.png","software":"SREFLEX","pdb_link":[{"pdb_code":"5oqq","ext_code":"5OQQ","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFD4_fit2_model1.pdb","model_mw":106.0,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/log_TC1Jeri.txt","symmetry":"P1","comment":null,"user":425}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/rc01_6iVq9ds_fixed_fit_img.png","software":"CRYSOL","chi_square_value":0.935,"p_value":0.0,"fit_residual_plot":"rc01_6iVq9ds_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFD4_fit2.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/rc01_KqVj408.log","software_version":"2.8.3","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"2.8.3","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the Ycg1-Brn1 complex in 25 mM Tris 300 mM NaCl 1mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 5 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.","tags":[],"intensity_unit":null,"experimental_mw":138.0,"experimental_mw_error":null,"guinier_i0_mw":122.0,"guinier_i0_mw_error":null,"porod_mw":144.0,"porod_mw_error":null,"pddf_i0":9503.0,"pddf_i0_error":null,"guinier_i0":9640.0,"guinier_i0_error":55.0,"pddf_rg":4.186,"pddf_rg_error":null,"guinier_rg":4.25,"guinier_rg_error":0.1,"pddf_dmax":13.73,"pddf_dmax_error":null,"porod_volume":230.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":24,"guinier_point_last":122,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFD4_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}