{"code":"SASDFG5","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases.","publication":{"title":"Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases.","author_list":"Dos Santos Morais R, Santo PE, Ley M, Schelcher C, Abel Y, Plassart L, Deslignière E, Chagot ME, Quinternet M, Paiva ACF, Hessmann S, Morellet N, M F Sousa P, Vandermoere F, Bertrand E, Charpentier B, Bandeiras TM, Plisson-Chastang C, Verheggen C, Cianférani S, Manival X","journal":"J Mol Biol","doi":"10.1016/j.jmb.2022.167760","pmid":"35901867","published_date":"2022 Jul 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TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"His-RuvBl1/RuvBl2 dodecamer","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"France","full_name":"University of Lorraine","webpage":"http://welcome.univ-lorraine.fr/"}],"contributor_name":"Raphael","contributor_surname":"Dos Santos Morais","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2019-03-21","storage_temperature":null,"cell_temperature":15.0,"exposure_time":1.0,"number_of_frames":17,"wavelength":0.1033,"sample_detector_distance":2.0,"concentration_min":null,"concentration_max":6.0,"sample_volume":null,"flow_rate":null,"s_min":0.041,"s_max":5.786,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFG5_fit1_model1_img.png","software":"DAMMIF","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFG5_fit1_model1.pdb","model_mw":644.0,"bead_radius":3.3,"log":null,"symmetry":null,"comment":null,"user":427}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDFG5_fit1_fixed_fit_img.png","software":"DAMMIF","chi_square_value":5.098,"p_value":6e-06,"fit_residual_plot":"SASDFG5_fit1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFG5_fit1.fir","fit_log":null,"software_version":null,"description":""},{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFG5_fit2_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFG5_fit2_model1.pdb","model_mw":null,"bead_radius":1.9,"log":null,"symmetry":"P62","comment":null,"user":427}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/SASDFG5_fit2_fixed_fit_img.png","software":"GASBOR","chi_square_value":1.54,"p_value":0.0008,"fit_residual_plot":"SASDFG5_fit2_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFG5_fit2.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The experimental molecular weight was determined from SEC-RALS/LALS experiment (670 kDa).","experiment_description":"Synchrotron SAXS data from solutions of the His-RuvBl1/RuvBl2 dodecamer in 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH 7.5 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the SWING beam line at SOLEIL (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.1033 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 15°C using the following parameters: Column: BioSEC5-500A (4.6 mm id * 300 mm); Flow rate: 0.3 mL/min; Sample injection concentration: 6 mg/mL; Injection volume: 50 μL. The data were collected through the SEC peak of the protein as a series of 17 x 1 second exposures. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":670.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.1974,"pddf_i0_error":null,"guinier_i0":0.1979,"guinier_i0_error":0.0001,"pddf_rg":6.08,"pddf_rg_error":null,"guinier_rg":6.15,"guinier_rg_error":0.06,"pddf_dmax":20.0,"pddf_dmax_error":null,"porod_volume":1490.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":6,"guinier_point_last":38,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFG5_datloglog_img.png","symmetry":null,"last_modified":"2022-08-08T12:00:28.219845+02:00","bragg_peak":[]}