{"code":"SASDFJ3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Solution Structure of SpoIVB Reveals Mechanism of PDZ Domain-Regulated Protease Activity.","publication":{"title":"Solution Structure of SpoIVB Reveals Mechanism of PDZ Domain-Regulated Protease Activity.","author_list":"Xie X, Guo N, Xue G, Xie D, Yuan C, Harrison J, Li J, Jiang L, Huang M","journal":"Front Microbiol","doi":"10.3389/fmicb.2019.01232","pmid":"31244791","published_date":"2019"},"status":"released","submitted_date":"2019-02-27","released_date":"2019-05-15"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFJ3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFJ3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFJ3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFJ3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFJ3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFJ3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFJ3.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Shanghai Synchrotron Radiation Facility (SSRF)","city":"Shanghai","country":"China","beamline_name":"BL19U2","beam_geometry":"","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"SpoIVB peptidase (MBP 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peptidase fusion (MBP-SpoIVB)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"China","full_name":"Fuzhou University","webpage":"https://www.fzu.edu.cn/"}],"contributor_name":"Longguang","contributor_surname":"Jiang","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-07-12","storage_temperature":4.0,"cell_temperature":10.0,"exposure_time":1.0,"number_of_frames":20,"wavelength":0.103,"sample_detector_distance":2.3,"concentration_min":null,"concentration_max":5.0,"sample_volume":null,"flow_rate":null,"s_min":0.07,"s_max":3.348,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFJ3_fit1_model1_img.png","software":"Chimera","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFJ3_fit1_model1.pdb","model_mw":79.22,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":422}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/IVB-5mg_1_fit_img.png","software":"DAMMIN","chi_square_value":0.971,"p_value":0.330455,"fit_residual_plot":"IVB-5mg_1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFJ3_fit1.fir","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The originally deposited p(r) profile is included in the full entry zip archive.","experiment_description":"Synchrotron SAXS data from solutions of MBP-SpoIVB peptidase fusion protein in 20 mM Tris-HCl, 150 mM NaCl, 5% glycerol, pH 8 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF, Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 2.3 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 10°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":77.6,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":60.0,"porod_mw_error":null,"pddf_i0":28.57,"pddf_i0_error":null,"guinier_i0":27.64,"guinier_i0_error":0.2,"pddf_rg":4.04,"pddf_rg_error":null,"guinier_rg":3.72,"guinier_rg_error":0.05,"pddf_dmax":15.6,"pddf_dmax_error":null,"porod_volume":96.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":33,"guinier_point_last":72,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFJ3_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}