{"code":"SASDFK4","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1.","publication":{"title":"Solution structure and flexibility of the condensin HEAT-repeat subunit Ycg1.","author_list":"Manalastas-Cantos K, Kschonsak M, Haering CH, Svergun DI","journal":"J Biol Chem","doi":"10.1074/jbc.RA119.008661","pmid":"31350339","published_date":"2019 Sep 13"},"status":"released","submitted_date":"2019-03-20","released_date":"2019-07-26"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFK4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFK4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFK4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFK4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFK4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFK4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFK4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"PETRA III","city":"DESY; Hamburg","country":"Germany","beamline_name":"EMBL P12","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Condensin complex subunit 3-like protein","short_name":"Ycg1","sequence":">tr|G0SHK2|G0SHK2_CHATD Condensin complex subunit 3-like protein OS=Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) 24-1006\r\nPDNALRTQVTTVFREAQRTTASHRKLVVTLRKIQEACCYEPATGQKQSGAAAALSEFDED\r\nDFNTEFVRCALRILPVKKSEGVGEKTVRFVSLFLRHAIEKDNELLGDTQMDEDDADNSTM\r\nPETPSTRLTSHLMETILPMLMAKDKFVRYRAAQLLAHIVNSLDAIDDDLYQKLRAGLLRR\r\nIHDKEPMVRAQAVLGLGRLAGNALDEEPGAGDSDLDGDDDGPGGASGVSLMDKLMDVMKN\r\nDPSADVRRTLLVNLPITPKTLPSLLERARDQDPATRRAVYSRLLPALGDFRHLSLSMREK\r\nLLRWGLRDRDENVRKAAARLFRERWIEDCAGTPRPEPGQPIELSPPNMDALLELLERIDV\r\nVNSGGENGIALEAMRGFWEGRPDYRDAMVFDDHFFETLSAESVFALRTFNDFCRNEAGGK\r\nYEALIEEKLPEVTKLAFYLERYIKVLIDAIKRAEEQGEEEEEDTAEQEFIVEQLLHIALT\r\nLDYADEVGRRKMFALLRQTLSIPELPEECTKLTVMVLRDLCAPDAAGEKEFTSVVLEAVA\r\nDVHDTIVDEDDDDANVPAEDDESFVSARSEVSRESTPAPGGGEGGGSNSRKRGRSPTPKM\r\nTEEEAAAKAIREIMINMKCLHIVQCMLQNVQGQLQSNDNLISMLNNLVVPAVRSHEAPVR\r\nERGLVCLGLCALLDRSLAEENLTLFIHFFAKGHTALQITALHILTDILVVHGAQLLSANP\r\nTLLKVYVKALRSGAKHPELQAVAVVAASKLLLGRVVSDRDVAAELLKTLVVAYFEPASAG\r\nNQSVRQALNYFLPVFCYSRAENQDLMRRVALDALHTLYNVREGFDDDDADIDDEMVSLAT\r\nIGACLVDWTDPRKVYTPDAGGLQAEKHVNADVHLDFAKAILERLEGNVPREEKKVLASLL\r\nSKLYISPASSPDKTRETYRVVSAAVDTNLLPDTTSRNALYKIHVSLGKIVNALDGAVPSV\r\nETASVAGSTGTGIGTTTTAATGR","organism":"Chaetomium 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24-1006\r\nPDNALRTQVTTVFREAQRTTASHRKLVVTLRKIQEACCYEPATGQKQSGAAAALSEFDED\r\nDFNTEFVRCALRILPVKKSEGVGEKTVRFVSLFLRHAIEKDNELLGDTQMDEDDADNSTM\r\nPETPSTRLTSHLMETILPMLMAKDKFVRYRAAQLLAHIVNSLDAIDDDLYQKLRAGLLRR\r\nIHDKEPMVRAQAVLGLGRLAGNALDEEPGAGDSDLDGDDDGPGGASGVSLMDKLMDVMKN\r\nDPSADVRRTLLVNLPITPKTLPSLLERARDQDPATRRAVYSRLLPALGDFRHLSLSMREK\r\nLLRWGLRDRDENVRKAAARLFRERWIEDCAGTPRPEPGQPIELSPPNMDALLELLERIDV\r\nVNSGGENGIALEAMRGFWEGRPDYRDAMVFDDHFFETLSAESVFALRTFNDFCRNEAGGK\r\nYEALIEEKLPEVTKLAFYLERYIKVLIDAIKRAEEQGEEEEEDTAEQEFIVEQLLHIALT\r\nLDYADEVGRRKMFALLRQTLSIPELPEECTKLTVMVLRDLCAPDAAGEKEFTSVVLEAVA\r\nDVHDTIVDEDDDDANVPAEDDESFVSARSEVSRESTPAPGGGEGGGSNSRKRGRSPTPKM\r\nTEEEAAAKAIREIMINMKCLHIVQCMLQNVQGQLQSNDNLISMLNNLVVPAVRSHEAPVR\r\nERGLVCLGLCALLDRSLAEENLTLFIHFFAKGHTALQITALHILTDILVVHGAQLLSANP\r\nTLLKVYVKALRSGAKHPELQAVAVVAASKLLLGRVVSDRDVAAELLKTLVVAYFEPASAG\r\nNQSVRQALNYFLPVFCYSRAENQDLMRRVALDALHTLYNVREGFDDDDADIDDEMVSLAT\r\nIGACLVDWTDPRKVYTPDAGGLQAEKHVNADVHLDFAKAILERLEGNVPREEKKVLASLL\r\nSKLYISPASSPDKTRETYRVVSAAVDTNLLPDTTSRNALYKIHVSLGKIVNALDGAVPSV\r\nETASVAGSTGTGIGTTTTAATGR","organism":"Chaetomium thermophilum","uniprot_code":"G0SHK2","uniprot_range_first":24,"uniprot_range_last":1006,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MPPRTRSTRSSRSSSAIDIPEETPDNALRTQVTTVFREAQRTTASHRKLVVTLRKIQEAC\nCYEPATGQKQSGAAAALSEFDEDDFNTEFVRCALRILPVKKSEGVGEKTVRFVSLFLRHA\nIEKDNELLGDTQMDEDDADNSTMPETPSTRLTSHLMETILPMLMAKDKFVRYRAAQLLAH\nIVNSLDAIDDDLYQKLRAGLLRRIHDKEPMVRAQAVLGLGRLAGNALDEEPGAGDSDLDG\nDDDGPGGASGVSLMDKLMDVMKNDPSADVRRTLLVNLPITPKTLPSLLERARDQDPATRR\nAVYSRLLPALGDFRHLSLSMREKLLRWGLRDRDENVRKAAARLFRERWIEDCAGTPRPEP\nGQPIELSPPNMDALLELLERIDVVNSGGENGIALEAMRGFWEGRPDYRDAMVFDDHFFET\nLSAESVFALRTFNDFCRNEAGGKYEALIEEKLPEVTKLAFYLERYIKVLIDAIKRAEEQG\nEEEEEDTAEQEFIVEQLLHIALTLDYADEVGRRKMFALLRQTLSIPELPEECTKLTVMVL\nRDLCAPDAAGEKEFTSVVLEAVADVHDTIVDEDDDDANVPAEDDESFVSARSEVSRESTP\nAPGGGEGGGSNSRKRGRSPTPKMTEEEAAAKAIREIMINMKCLHIVQCMLQNVQGQLQSN\nDNLISMLNNLVVPAVRSHEAPVRERGLVCLGLCALLDRSLAEENLTLFIHFFAKGHTALQ\nITALHILTDILVVHGAQLLSANPTLLKVYVKALRSGAKHPELQAVAVVAASKLLLGRVVS\nDRDVAAELLKTLVVAYFEPASAGNQSVRQALNYFLPVFCYSRAENQDLMRRVALDALHTL\nYNVREGFDDDDADIDDEMVSLATIGACLVDWTDPRKVYTPDAGGLQAEKHVNADVHLDFA\nKAILERLEGNVPREEKKVLASLLSKLYISPASSPDKTRETYRVVSAAVDTNLLPDTTSRN\nALYKIHVSLGKIVNALDGAVPSVETASVAGSTGTGIGTTTTAATGRRVTRGSVSAGSGTG\nAGRRSISRASSVSTNADRPASASAAVPGSKAASTIEEEEEKEQEEEEEEEEKEEKEEEEE\nEEEEENVDEETQLAMQLQADTTAAQSREGSVQIKREEDDSESEASDATVVRGSVGTGRRV\nKQEEDDDF","mw":108.3,"total_mw":216.6,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"25 mM Tris, 300 mM NaCl, 1mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Condensin complex subunit 3-like protein, Ycg1, at 0.25mg/ml","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"EMBL-Hamburg","address":"Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany","full_name":"European Molecular Biology Laboratory (EMBL) - Hamburg outstation","webpage":"http://www.embl-hamburg.de/index.php"}],"contributor_name":"Karen","contributor_surname":"Manalastas-Cantos","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2016-10-20","storage_temperature":13.1,"cell_temperature":10.1,"exposure_time":0.05,"number_of_frames":20,"wavelength":0.124,"sample_detector_distance":4.0,"concentration_min":null,"concentration_max":0.25,"sample_volume":null,"flow_rate":null,"s_min":0.049,"s_max":2.515,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFK4_fit1_model1_img.png","software":"SASREF MX","pdb_link":[{"pdb_code":"5oqq","ext_code":"5OQQ","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFK4_fit1_model1.pdb","model_mw":188.7,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/HR_conc_seriesB_p2_2_tnYM8oG.log","symmetry":"P2","comment":"Dimer is 42% of solution","user":425},{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFK4_fit1_model2_img.png","software":"SREFLEX","pdb_link":[{"pdb_code":"5oqq","ext_code":"5OQQ","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":"2.8.3","model_data":"https://www.sasbdb.org/media/pdb_file/SASDFK4_fit1_model2.pdb","model_mw":94.36,"bead_radius":null,"log":null,"symmetry":"P1","comment":"Monomer is 58% of solution","user":425}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/1766_B_0p25_buffB_0165_crop_fixed_fit_img.png","software":"SASREF MX","chi_square_value":1.09,"p_value":0.008895,"fit_residual_plot":"1766_B_0p25_buffB_0165_crop_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFK4_fit1.fit","fit_log":null,"software_version":"2.8.3","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"2.8.3","i0_calibration_standard":null,"description":"Ycg1 at 0.25 mg/ml was modeled as a volume-fraction weighted mixture consisting of 66% monomers and 34% dimers","experiment_description":"Synchrotron SAXS data from solutions of Ycg1 in 25 mM Tris 300 mM NaCl 1mM DTT, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.25 mg/ml was measured at 10.1°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":null,"experimental_mw":119.0,"experimental_mw_error":null,"guinier_i0_mw":138.0,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":10710.0,"pddf_i0_error":null,"guinier_i0":10748.0,"guinier_i0_error":70.0,"pddf_rg":4.645,"pddf_rg_error":null,"guinier_rg":4.64,"guinier_rg_error":0.28,"pddf_dmax":15.01,"pddf_dmax_error":null,"porod_volume":280.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":12,"guinier_point_last":112,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFK4_datloglog_img.png","symmetry":null,"last_modified":"2023-06-21T07:56:39.492710+02:00","bragg_peak":[]}