{"code":"SASDFM3","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Histone chaperone exploits intrinsic disorder to switch acetylation specificity.","publication":{"title":"Histone chaperone exploits intrinsic disorder to switch acetylation specificity.","author_list":"Danilenko N, Lercher L, Kirkpatrick J, Gabel F, Codutti L, Carlomagno T","journal":"Nat Commun","doi":"10.1038/s41467-019-11410-7","pmid":"31387991","published_date":"2019 Aug 6"},"status":"released","submitted_date":"2019-02-27","released_date":"2019-07-10"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFM3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM3_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFM3.sascif","experiment":{"instrument":{"detector":{"type":"SANS","name":"6Li-Scintillator 1 mm thickness + photomultiplier","resolution":5.3},"name":"FRM2","city":"Munich","country":"Germany","beamline_name":"KWS1","beam_geometry":null,"type_of_source":"neutron source","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Vacuolar protein sorting-associated protein 75 (1-225 aa)","short_name":"Vps75 1-225","sequence":"MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI\r\nVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ\r\nVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF\r\nGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLED","organism":"Saccharomyces 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RTT109","short_name":"Rtt109","sequence":"MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK\r\nVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL\r\nQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT\r\nCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA\r\nKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE\r\nDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA\r\nIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA\r\nITMLKPRKKAKALPKT","organism":"Saccharomyces 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ASF1","short_name":"Asf1","sequence":"MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI\r\nLVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE\r\nELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGV","organism":null,"uniprot_code":"P32447","uniprot_range_first":1,"uniprot_range_last":169,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI\nLVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE\nELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEE\nDDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGE\nEEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN","mw":19.125,"total_mw":19.125,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Histone H3.2 (35-135 aa)","short_name":"H3 (35-135)","sequence":"VKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAY \r\nLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA","organism":"Xenopus laevis","uniprot_code":"P84233","uniprot_range_first":35,"uniprot_range_last":135,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE\nLLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTI\nMPKDIQLARRIRGERA","mw":11.747,"total_mw":11.747,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Histone H4","short_name":"H4","sequence":"MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK\r\nVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG","organism":"Xenopus laevis","uniprot_code":"P62799","uniprot_range_first":1,"uniprot_range_last":103,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK\nVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG","mw":11.367,"total_mw":11.367,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM citrate, 150 mM NaCl, 5 mM BME, 100% D2O","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.5,"deuteration":null},"purity_method":null,"name":"Complex with 1H histone chaperone Asf1 and histones H3 and H4, 2H histone acetyltransferase Rtt109 and histone chaperone Vps75 (1H Asf1-H3:H4, 2H Rtt109-Vps75) acquired in 100% v/v D2O","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Germany","full_name":"Leibniz University of Hannover","webpage":"https://www.uni-hannover.de"}],"contributor_name":"Nataliya","contributor_surname":"Danilenko","orcid":"https://orcid.org/0000-0003-4885-5051"}],"concentration_method":null,"concentration_unit":null,"date":"2017-03-04","storage_temperature":25.0,"cell_temperature":25.0,"exposure_time":21600.0,"number_of_frames":null,"wavelength":0.5,"sample_detector_distance":4.0,"concentration_min":3.85,"concentration_max":3.85,"sample_volume":null,"flow_rate":null,"s_min":0.127,"s_max":3.455,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFM3_fit1_model1_img.png","software":"HADDOCK","pdb_link":[{"pdb_code":"6o22","ext_code":"6O22","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFM3_fit1_model1.pdb","model_mw":144.0,"bead_radius":null,"log":"https://www.sasbdb.org/media/log_files/new190718_12003.log","symmetry":null,"comment":null,"user":420}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/new190718_12003_fit_img.png","software":"CRYSON","chi_square_value":15.132,"p_value":0.0,"fit_residual_plot":null,"fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFM3_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/new190718_12003.log","software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":null,"experiment_description":"SANS data from solutions of the Asf1-H3:H4-Rtt109-Vps75 protein complex (protonated Asf1-H3:H4 bound to perdeuterated Rtt109-Vps75) in 50 mM citrate, 150 mM NaCl, 5 mM BME, 100% D2O, pD 6.5 were collected on the KWS1 instrument at FRM2 (Munich, Germany) using a SANS 6Li-Scintillator 1 mm thickness + photomultiplier detector. Due to a combination of negative (from 1H Asf1-H3:H4) and positive (from perdeuterated Rtt109-Vps75) contrasts the Rg obtained from the Guinier approximation is negative. The data were recorded at two detector configurations: 1) 4 m for 6 hrs using a neutron wavelength of 0.5 nm and; 2) 1.5 m for 2.5 hrs using a neutron wavelength of 0.5 nm. Both measurement were performed on the same sample at 3.85 mg/mL, measured at 25°C.","tags":[],"intensity_unit":null,"experimental_mw":145.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":0.017,"guinier_i0_error":0.0063,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":-2.81,"guinier_rg_error":0.327,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":3,"guinier_point_last":10,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFM3_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}