{"code":"SASDFM5","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Quaternary structure of α-amino-β-carboxymuconate-ϵ-semialdehyde decarboxylase (ACMSD) controls its activity.","publication":{"title":"Quaternary structure of α-amino-β-carboxymuconate-ϵ-semialdehyde decarboxylase (ACMSD) controls its activity.","author_list":"Yang Y, Davis I, Matsui T, Rubalcava I, Liu A","journal":"J Biol Chem","doi":"10.1074/jbc.RA119.009035","pmid":"31189654","published_date":"2019 Jul 26"},"status":"released","submitted_date":"2019-04-23","released_date":"2019-06-19"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFM5.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFM5.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM5_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM5_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFM5_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM5_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFM5.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus3 X 1M","resolution":0.172},"name":"Stanford Synchrotron Radiation Lightsource (SSRL)","city":"Menlo Park, CA","country":"USA","beamline_name":"BL4-2","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"2-amino-3-carboxymuconate 6-semialdehyde decarboxylase","short_name":"ACMSD","sequence":"MGHHHHHHHHHHSSGHIEGRH\r\nMKKPRIDMHSHFFPRISEQEAAKFDANHAPWLQVSAKGDTGSIMMGKNNFRPVYQALWDPAFRIEEMDAQGVDVQVTCAT\r\nPVMFGYTWEANKAAQWAERMNDFALEFAAHNPQRIKVLAQVPLQDLDLACKEASRAVAAGHLGIQIGNHLGDKDLDDATL\r\nEAFLTHCANEDIPILVHPWDMMGGQRMKKWMLPWLVAMPAETQLAILSLILSGAFERIPKSLKICFGHGGGSFAFLLGRV\r\nDNAWRHRDIVREDCPRPPSEYVDRFFVDSAVFNPGALELLVSVMGEDRVMLGSDYPFPLGEQKIGGLVLSSNLGESAKDK\r\nIISGNASKFFNINV","organism":"Pseudomonas fluorescens","uniprot_code":"Q83V25","uniprot_range_first":1,"uniprot_range_last":334,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":"MKKPRIDMHSHFFPRISEQEAAKFDANHAPWLQVSAKGDTGSIMMGKNNFRPVYQALWDP\nAFRIEEMDAQGVDVQVTCATPVMFGYTWEANKAAQWAERMNDFALEFAAHNPQRIKVLAQ\nVPLQDLDLACKEASRAVAAGHLGIQIGNHLGDKDLDDATLEAFLTHCANEDIPILVHPWD\nMMGGQRMKKWMLPWLVAMPAETQLAILSLILSGAFERIPKSLKICFGHGGGSFAFLLGRV\nDNAWRHRDIVREDCPRPPSEYVDRFFVDSAVFNPGALELLVSVMGEDRVMLGSDYPFPLG\nEQKIGGLVLSSNLGESAKDKIISGNASKFFNINV","mw":39.661,"total_mw":158.644,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Tris, 5 mM DTT","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.5,"deuteration":null},"purity_method":null,"name":"Mutant 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase, H110A tetramer, at pH 8.5","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"San Antonia, TX, USA","full_name":"University of Texas at San Antonio","webpage":"https://www.utsa.edu/"}],"contributor_name":"Yu","contributor_surname":"Yang","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-07-15","storage_temperature":25.0,"cell_temperature":25.0,"exposure_time":1.0,"number_of_frames":500,"wavelength":0.1228,"sample_detector_distance":2.5,"concentration_min":null,"concentration_max":null,"sample_volume":0.05,"flow_rate":null,"s_min":0.14,"s_max":3.003,"total_exposure_time":null,"seccolumn":2},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFM5_fit1_model1_img.png","software":"CORAL","pdb_link":[],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFM5_fit1_model1.pdb","model_mw":157.4,"bead_radius":1.9,"log":null,"symmetry":null,"comment":null,"user":440}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/07-P2_Dimersr00_fixed_fit_img.png","software":"CORAL","chi_square_value":0.25,"p_value":0.063313,"fit_residual_plot":"07-P2_Dimersr00_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFM5_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of mutant ACMSD (H110A) in 50 mM Tris, 5 mM DTT, pH 8.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1228 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 0.05 ml sample at  mg/ml was injected onto a GE Superdex 200 Increase 5/150 column at 25°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":158.8,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":148.8,"porod_mw_error":null,"pddf_i0":13.77,"pddf_i0_error":0.19,"guinier_i0":13.309,"guinier_i0_error":0.376,"pddf_rg":5.6,"pddf_rg_error":0.08,"guinier_rg":5.22,"guinier_rg_error":0.195,"pddf_dmax":19.0,"pddf_dmax_error":null,"porod_volume":238.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":31,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFM5_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}