{"code":"SASDFM6","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/nm","project":{"title":"Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.","publication":{"title":"Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.","author_list":"Narayanan N, Banerjee A, Jain D, Kulkarni DS, Sharma R, Nirwal S, Rao DN, Nair DT","journal":"J Mol Biol","doi":"10.1016/j.jmb.2019.10.001","pmid":"31628946","published_date":"2019 Oct 16"},"status":"released","submitted_date":"2019-05-31","released_date":"2019-11-17"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFM6.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFM6.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM6_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM6_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFM6_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFM6_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFM6.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"ESRF","city":"Grenoble","country":"France","beamline_name":"BM29","beam_geometry":"","type_of_source":"X-ray 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NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":5.5,"deuteration":null},"purity_method":null,"name":"Adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"RCB","address":"Haryana, India","full_name":"Regional Centre for Biotechnology","webpage":"http://www.rcb.res.in/"}],"contributor_name":"Naveen","contributor_surname":"Narayanan","orcid":"https://orcid.org/0000-0001-8327-1559"}],"concentration_method":null,"concentration_unit":null,"date":"2018-12-01","storage_temperature":null,"cell_temperature":null,"exposure_time":null,"number_of_frames":null,"wavelength":null,"sample_detector_distance":null,"concentration_min":null,"concentration_max":2.0,"sample_volume":null,"flow_rate":null,"s_min":0.038,"s_max":4.946,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFM6_fit1_model1_img.png","software":"PyMOL","pdb_link":[],"model_title":null,"type_of_model":"atomic","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFM6_fit1_model1.pdb","model_mw":233.8,"bead_radius":null,"log":null,"symmetry":null,"comment":null,"user":451}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/tetramer_after_md00_fixed_fit_img.png","software":"CRYSOL","chi_square_value":1.796,"p_value":0.0,"fit_residual_plot":"tetramer_after_md00_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFM6_fit1.fit","fit_log":null,"software_version":null,"description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":"The sample is severely aggregated. Wavelength UNKNOWN; Experimental temperature, UNKNOWN; Exposure time, UNKNOWN; Sample to detector distance, UNKNOWN.","experiment_description":"Synchrotron SAXS data from solutions of adenine specific DNA methyltransferase (Mod HP_0593) at pH 5.5 in 25 mM citrate, 250 mM NaCl were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":250.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":209.0,"porod_mw_error":null,"pddf_i0":122.9,"pddf_i0_error":null,"guinier_i0":126.76,"guinier_i0_error":0.8,"pddf_rg":4.91,"pddf_rg_error":null,"guinier_rg":4.98,"guinier_rg_error":0.42,"pddf_dmax":19.1,"pddf_dmax_error":null,"porod_volume":316.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":18,"guinier_point_last":47,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFM6_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}