{"code":"SASDFP7","status":"Published","type_of_curve":"Single concentration","angular_unit":"1/A","project":{"title":"Histone chaperone exploits intrinsic disorder to switch acetylation specificity (Asf1-H3:H4-Rtt109-Vps75 protein complex, data for docking block selections)","publication":{"title":"Histone chaperone exploits intrinsic disorder to switch acetylation specificity (Asf1-H3:H4-Rtt109-Vps75 protein complex, data for docking block selections)","author_list":"Danilenko N, Lercher L, Kirkpatrick J, Gabel F, Codutti L, Carlomagno T","journal":"Nat Commun","doi":null,"pmid":null,"published_date":"2019 Aug 6"},"status":"released","submitted_date":"2019-06-17","released_date":"2019-07-10"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFP7.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFP7.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFP7_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFP7_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFP7_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFP7_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFP7.sascif","experiment":{"instrument":{"detector":{"type":"128 linear sensitive multidetector","name":"Reuter-Stokes 3He","resolution":0.8},"name":"Institut Laue-Langevin (ILL)","city":"Grenoble","country":"France","beamline_name":"D22","beam_geometry":null,"type_of_source":"neutron source","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Histone acetyltransferase RTT109","short_name":"Rtt109","sequence":"MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPKSTIKTQHFFSLFHQGK\r\nVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRDITKIILEFILSIDPNYYL\r\nQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGSTVLKEIESPRFQQDLYLSFT\r\nCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEELMKWWGFILDRLLIECFQNDTQA\r\nKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENSLAVYNIPLFPDDPKARFIHQLAEE\r\nDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISGYSLATPSLFPSSADVIVPKSRKQFRA\r\nIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMATNLQSLTGKREHRERNQPVPASNINTLA\r\nITMLKPRKKAKALPKT","organism":"Saccharomyces 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ASF1","short_name":"Asf1","sequence":"MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI\r\nLVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE\r\nELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGV","organism":null,"uniprot_code":"P32447","uniprot_range_first":1,"uniprot_range_last":169,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSI\nLVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEE\nELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEE\nDDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGE\nEEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN","mw":19.125,"total_mw":19.125,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Histone H4","short_name":"H4","sequence":"MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK\r\nVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG","organism":"Xenopus laevis","uniprot_code":"P62799","uniprot_range_first":1,"uniprot_range_last":103,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLK\nVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG","mw":11.367,"total_mw":11.367,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Histone H3 full-length","short_name":"H3 FL","sequence":"MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALR \r\nEIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAY \r\nLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA","organism":"Xenopus laevis","uniprot_code":"Q6PI79","uniprot_range_first":1,"uniprot_range_last":136,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTE\nLLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTI\nMPKDIQLARRIRGERA","mw":15.328,"total_mw":15.328,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null},{"long_name":"Vacuolar protein sorting-associated protein 75 full-length","short_name":"Vps75 (FL)","sequence":"MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI\r\nVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ\r\nVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF\r\nGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLEDEEGESGLSADGDSED\r\nDDGSLGEVDLPLSDEEPSSKKRKV","organism":"Saccharomyces cerevisiae","uniprot_code":"P53853","uniprot_range_first":1,"uniprot_range_last":264,"oligomerization":"dimer","molecular_type":"protein","uniprot_sequence":"MMSDQENENEHAKAFLGLAKCEEEVDAIEREVELYRLNKMKPVYEKRDAYIDEIAEFWKI\nVLSQHVSFANYIRASDFKYIDTIDKIKVEWLALESEMYDTRDFSITFHFHGIEGDFKEQQ\nVTKVFQIKKGKDDQEDGILTSEPVPIEWPQSYDSINPDLIKDKRSPEGKKKYRQGMKTIF\nGWFRWTGLKPGKEFPHGDSLASLFSEEIYPFCVKYYAEAQRDLEDEEGESGLSADGDSED\nDDGSLGEVDLPLSDEEPSSKKRKV","mw":30.615,"total_mw":61.23,"number_molecules":2,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM citrate, 150 mM NaCl, 5 mM BME, 100% D2O","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":6.5,"deuteration":null},"purity_method":null,"name":"Complex with all 1H histone acetyltransferase Rtt109 with histones H3 and H4 and histone chaperones Asf1 and Vps75, all full-length, acquired in 100% v/v D2O","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Germany","full_name":"Leibniz University of Hannover","webpage":"https://www.uni-hannover.de"}],"contributor_name":"Nataliya","contributor_surname":"Danilenko","orcid":"https://orcid.org/0000-0003-4885-5051"}],"concentration_method":null,"concentration_unit":null,"date":"2016-06-09","storage_temperature":null,"cell_temperature":25.0,"exposure_time":7200.0,"number_of_frames":1,"wavelength":0.6,"sample_detector_distance":4.0,"concentration_min":null,"concentration_max":2.73,"sample_volume":null,"flow_rate":null,"s_min":0.142,"s_max":2.398,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"4.6","i0_calibration_standard":null,"description":null,"experiment_description":"SANS data from solutions the all 1H Asf1-H3:H4-Rtt109-Vps75 protein complex in 50 mM citrate, 150 mM NaCl, 5 mM BME, 100% D2O, pD 6.5 were collected on the D22 instrument at the ILL (Grenoble, France) using a 3He multidetector 128 linear sensitive Reuter-Stokes detector detector at a sample-detector/Collimation distance of 4 m/4 m and at a wavelength of λ = 0.6 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.73 mg/ml was measured at 25°C for 2 h.","tags":[],"intensity_unit":"arbitrary","experimental_mw":157.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.3735,"pddf_i0_error":null,"guinier_i0":0.3754,"guinier_i0_error":0.0008,"pddf_rg":3.45,"pddf_rg_error":null,"guinier_rg":3.496,"guinier_rg_error":0.04,"pddf_dmax":11.5,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":11,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFP7_datloglog_img.png","symmetry":null,"last_modified":"2022-08-15T14:51:04.900446+02:00","bragg_peak":[]}