{"code":"SASDFQ4","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Conformationally constrained mutant of human matrix metalloproteinase-1","publication":null,"status":"released","submitted_date":"2019-03-26","released_date":"2021-09-27"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFQ4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFQ4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFQ4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFQ4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFQ4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFQ4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFQ4.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Pilatus 2M","resolution":null},"name":"Diamond Light Source","city":"Didcot","country":"UK","beamline_name":"B21","beam_geometry":"1 x 5 mm","type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Pro-matrix metalloproteinase-1 (Interstitial collagenase) (proMMP-1 S243C, S318C)","short_name":"ProMMP-1 (S243C, S31","sequence":"MFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPV\r\nVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIEN\r\nYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGN\r\nLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSY\r\nTFCGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDR\r\nFYMRTNPFYPEVELNFICVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGY\r\nPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFP\r\nGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN","organism":"Homo sapiens","uniprot_code":"P03956","uniprot_range_first":20,"uniprot_range_last":469,"oligomerization":"monomer","molecular_type":"protein","uniprot_sequence":"MHSFPPLLLLLFWGVVSHSFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPV\nVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIEN\nYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGN\nLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSY\nTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDR\nFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGY\nPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFP\nGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN","mw":52.007,"total_mw":52.007,"number_molecules":1,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50 mM Tris-HCl, 150 mM Sodium chloride, 10 mM Calcium chloride","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.4,"deuteration":null},"purity_method":null,"name":"Domain-stapled mutant of Pro-Matrix metalloproteinase-1 (proMMP-1 S243C, S318C)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Portsmouth, UK","full_name":"University of Portsmouth","webpage":"https://www.port.ac.uk"}],"contributor_name":"Rob","contributor_surname":"Holland","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2015-05-21","storage_temperature":4.0,"cell_temperature":25.0,"exposure_time":3.0,"number_of_frames":28,"wavelength":0.1,"sample_detector_distance":3.9,"concentration_min":0.0,"concentration_max":5.6,"sample_volume":null,"flow_rate":null,"s_min":0.033,"s_max":4.038,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of the domain-stapled mutant of pro-matrix metalloproteinase-1 (proMMP-1 S243C, S318C) in 50 mM Tris-HCl, 150 mM NaCl, 10 mM CaCl2, pH 7.4 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 25 °C using the following parameters: Shodex Kw-403 analytical SEC column; Flow rate: 0.2 mL/min; Sample injection concentration: 5.6 mg/mL; Injection volume: 50 μL. The data were collected through the SEC peak of the sample as a series of 28 x 3 second exposures. Each unsubtracted data frame was normalised to the intensity of the transmitted beam and radially averaged and the scattering of an appropriate solvent-blank was subtracted. The resulting subtracted frames were scaled and averaged to generate the final SAXS profile displayed in this entry.","tags":[],"intensity_unit":"arbitrary","experimental_mw":39.35,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":38.0,"porod_mw_error":null,"pddf_i0":0.01028,"pddf_i0_error":null,"guinier_i0":0.01029,"guinier_i0_error":5.1e-05,"pddf_rg":2.71,"pddf_rg_error":null,"guinier_rg":2.694,"guinier_rg_error":0.021,"pddf_dmax":8.9,"pddf_dmax_error":null,"porod_volume":61.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":35,"guinier_point_last":164,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFQ4_datloglog_img.png","symmetry":null,"last_modified":"2021-09-27T16:14:08.329705+02:00","bragg_peak":[]}