{"code":"SASDFR2","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.","publication":{"title":"Higher order assembling of the mycobacterial polar growth factor DivIVA/Wag31.","author_list":"Choukate K, Gupta A, Basu B, Virk K, Ganguli M, Chaudhuri B","journal":"J Struct Biol","doi":"10.1016/j.jsb.2019.107429","pmid":"31778770","published_date":"2019 Nov 25"},"status":"released","submitted_date":"2019-01-30","released_date":"2020-01-09"},"pddf_data":null,"intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFR2.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR2_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR2_kratky_img.png","pddf_plot":null,"intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR2_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFR2.sascif","experiment":{"instrument":{"detector":{"type":null,"name":"Rayonix MX225-HE","resolution":null},"name":"Stanford Synchrotron Radiation Lightsource (SSRL)","city":"Menlo Park, CA","country":"USA","beamline_name":"BL4-2","beam_geometry":null,"type_of_source":"X-ray synchrotron","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"Cell wall synthesis protein Wag31","short_name":null,"sequence":"MHHHHHHENLYFQGPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL\r\nAAGGGAGVTPQAEQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT\r\nAKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA\r\nDALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA\r\nAPVDSNADAGGFDQFNRGKN","organism":"Mycobacterium tuberculosis","uniprot_code":"P9WMU1","uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"unknown","molecular_type":"protein","uniprot_sequence":"MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQEL\nAAGGGAGVTPQATQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNT\nAKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKA\nDALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSA\nAPVDSNADAGGFDQFNRGKN","mw":29.98,"total_mw":29.98,"number_molecules":0,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME)","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":7.5,"deuteration":null},"purity_method":null,"name":"Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 275-279)","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":"CSIR","address":"Chandigarh, India","full_name":"CSIR-Institute of Microbial Technology","webpage":"http://www.imtech.res.in/"}],"contributor_name":"Komal","contributor_surname":"Choukate","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2018-01-29","storage_temperature":23.0,"cell_temperature":23.0,"exposure_time":1.0,"number_of_frames":500,"wavelength":0.1,"sample_detector_distance":2.54,"concentration_min":null,"concentration_max":2.4,"sample_volume":null,"flow_rate":null,"s_min":0.055,"s_max":4.023,"total_exposure_time":null,"seccolumn":null},"fits":[],"estimated_volume_method":null,"pddf_software":null,"pddf_software_version":null,"i0_calibration_standard":null,"description":"SEC-SAXS was performed at room temperature using the following parameters: Column: Superdex 200 PC3.2; Flow rate: 0.05 mL/min; Sample injection concentration: 2.4 mg/mL; Injection volume: 100 μL. 500 successive 1 second frames were collected through the SEC-elution peak.\r\nSEC-SAXS data of Cell wall synthesis protein Wag31(T73A) polymer frame no. 275-279","experiment_description":"Synchrotron SAXS data from solutions of Cell wall synthesis protein Wag31(T73A) polymer (SEC-frames 275-279) in 50mM Tris pH7.5, 300mM NaCl, 10% Glycerol, 1mM EDTA (ethylene diamine tetra acetic acid), 5mM β-mercaptoethanol (BME), pH 7.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL) storage ring (Stanford, CA, USA) using a Rayonix MX225-HE detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.40 mg/ml was measured at 23°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.","tags":[],"intensity_unit":"arbitrary","experimental_mw":29.92,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":null,"pddf_i0_error":null,"guinier_i0":43.5,"guinier_i0_error":null,"pddf_rg":null,"pddf_rg_error":null,"guinier_rg":15.22,"guinier_rg_error":null,"pddf_dmax":null,"pddf_dmax_error":null,"porod_volume":null,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":2,"guinier_point_last":7,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFR2_datloglog_img.png","symmetry":null,"last_modified":"2020-08-13T15:34:16.781383+02:00","bragg_peak":[]}