{"code":"SASDFR4","status":"Published","type_of_curve":"Extrapolated to infinite dilution","angular_unit":"1/A","project":{"title":"Construction of a Quadrangular Tetramer and a Cage-Like Hexamer from Three-Helix Bundle-Linked Fusion Proteins.","publication":{"title":"Construction of a Quadrangular Tetramer and a Cage-Like Hexamer from Three-Helix Bundle-Linked Fusion Proteins.","author_list":"Miyamoto T, Hayashi Y, Yoshida K, Watanabe H, Uchihashi T, Yonezawa K, Shimizu N, Kamikubo H, Hirota S","journal":"ACS Synth Biol","doi":"10.1021/acssynbio.9b00019","pmid":"30966743","published_date":"2019 Apr 16"},"status":"released","submitted_date":"2019-03-28","released_date":"2019-04-24"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFR4.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFR4.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR4_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR4_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFR4_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFR4_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFR4.sascif","experiment":{"instrument":{"detector":{"type":"Pilatus","name":"Pilatus 200K","resolution":172.0},"name":"Nara Institute of Science and Technology","city":"Ikoma","country":"Japan","beamline_name":"Rigaku Nano-Viewer","beam_geometry":null,"type_of_source":"X-ray in house","point_source":null,"line_collimation":null,"sample_path_length":null,"line_collimation_slitlength":null,"line_collimation_integrationwidth":null,"xray_energy":null,"beam_profile_ah":null,"beam_profile_al":null},"sample":{"molecule":[{"long_name":"HP2042 form from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, Trp repressor from Escherichia coli","short_name":"Fusion protein1","sequence":"MGSSHHHHHHSSGLVPRGSHMRDYSELEIFEGNPLDKWNDIIFHASKKASKKELERLLELLALCETFIEKEDLEEKFESFAKALRIDEELQQKIESRKTDIVIQSMANILSALFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYGSGSGRHQEWLRFVDLLKNAYQNDLHLPLLNLMLTPDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEEVLLKSD","organism":"Helicobacter pylori, Rattus norvegicus, Escherichia coli","uniprot_code":null,"uniprot_range_first":null,"uniprot_range_last":null,"oligomerization":"tetramer","molecular_type":"protein","uniprot_sequence":null,"mw":37.434,"total_mw":149.736,"number_molecules":4,"complex_state":false,"deuteration":null,"molecule_source":"biological","molecule_description":null}],"buffer":{"name":"20 mM Tris-HCl 150 mM NaCl","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"HP2042 form from Helicobacter pylori, N-terminal domain of syntaxin-1A from Rattus norvegicus, Trp repressor from Escherichia coli (Domain A (PDB:3MLI)-Linker (PDB:1BR0)-Domain B (PDB:1WRT))","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Japan","full_name":"Nara Institute of Science and Technology","webpage":"http://www.naist.jp/en/"}],"contributor_name":"Hironari","contributor_surname":"Kamikubo","orcid":"https://orcid.org/0000-0002-0878-6488"}],"concentration_method":null,"concentration_unit":null,"date":"2016-12-08","storage_temperature":4.0,"cell_temperature":20.0,"exposure_time":60.0,"number_of_frames":25,"wavelength":0.15418,"sample_detector_distance":0.75,"concentration_min":2.3,"concentration_max":8.3,"sample_volume":null,"flow_rate":null,"s_min":0.138,"s_max":3.003,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFR4_fit1_model1_img.png","software":"CORAL","pdb_link":[{"pdb_code":"3mli","ext_code":"3MLI","ext_type":"pdb","difference_with_model":"derived"},{"pdb_code":"1br0","ext_code":"1BR0","ext_type":"pdb","difference_with_model":"derived"},{"pdb_code":"1wrt","ext_code":"1WRT","ext_type":"pdb","difference_with_model":"derived"}],"model_title":null,"type_of_model":"mix","software_version":null,"model_data":"https://www.sasbdb.org/media/pdb_file/SASDFR4_fit1_model1.pdb","model_mw":148.0,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/tet401.log","symmetry":null,"comment":null,"user":432}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/tet401-1_fixed_fit_img.png","software":"CORAL","chi_square_value":0.1,"p_value":0.046149,"fit_residual_plot":"tet401-1_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFR4_fit1.fit","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/tet401.log","software_version":"5","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"SAXS data from solutions of the fusion protein1 in 20 mM Tris-HCl, 150 mM KCl, pH 8.0 were collected using a Nano-Viewer (Rigaku, Japan) at NAIST (Nara, Japan) equipped with a Pilatus 200K detector at a sample-detector distance of 0.75 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Twenty-five successive 60 second frames were collected at a sample temperature of 20°C. Solute concentrations ranging between 2.3 and 8.3 mg/ml were measured. The data were normalized to the intensity of the direct beam and radially averaged. The scattering from the solvent-blank was subtracted. The scattering profile in this entry represents an extrapolation to infinite dilution.","tags":[],"intensity_unit":"arbitrary","experimental_mw":177.0,"experimental_mw_error":null,"guinier_i0_mw":177.0,"guinier_i0_mw_error":4.0,"porod_mw":237.0,"porod_mw_error":null,"pddf_i0":37.39,"pddf_i0_error":0.9,"guinier_i0":37.24,"guinier_i0_error":1.5,"pddf_rg":5.24,"pddf_rg_error":2.0,"guinier_rg":5.11,"guinier_rg_error":0.3,"pddf_dmax":20.0,"pddf_dmax_error":null,"porod_volume":379.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":1,"guinier_point_last":10,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFR4_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}