{"code":"SASDFS3","status":"Published","type_of_curve":"SEC-SAS","angular_unit":"1/A","project":{"title":"A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family.","publication":{"title":"A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family.","author_list":"Sun Y, Keown JR, Black MM, Raclot C, Demarais N, Trono D, Turelli P, Goldstone DC","journal":"J Mol Biol","doi":"10.1016/j.jmb.2019.05.002","pmid":"31078555","published_date":"2019 May 9"},"status":"released","submitted_date":"2019-03-06","released_date":"2019-05-16"},"pddf_data":"https://www.sasbdb.org/media/p_of_R_files/SASDFS3.out","intensities_data":"https://www.sasbdb.org/media/intensities_files/SASDFS3.dat","intensities_log_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFS3_dat_img.png","intensities_kratky_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFS3_kratky_img.png","pddf_plot":"https://www.sasbdb.org/media/p_of_R_files/pofr_images/SASDFS3_pofr_img.png","intensities_guinier_plot":"https://www.sasbdb.org/media/intensities_files/scattering_plots/SASDFS3_guinier_img.png","sascif_data":"https://www.sasbdb.org/media/sascif/sascif_files/SASDFS3.sascif","experiment":{"instrument":{"detector":{"type":"Dectris","name":"Pilatus 1M","resolution":172.0},"name":"Australian  Synchrotron","city":"Melbourne","country":"Australia","beamline_name":"SAXS/WAXS","beam_geometry":"Point","type_of_source":"X-ray 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mM Tris 300 mM NaCl 0.1 mM TCEP","concentration_unit":null,"comment":null,"additive":null,"concentration":null,"pka":null,"ph":8.0,"deuteration":null},"purity_method":null,"name":"Murine transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D","ext_coefficient":null,"contrast":null,"specific_vol":null,"dry_vol":null,"absorbption":null,"deuteration":null,"mixture":null},"contributor":[{"affiliation":[{"short_name":null,"address":"Auckland, New Zealand","full_name":"The University of Auckland","webpage":"https://www.auckland.ac.nz"}],"contributor_name":"Yunyuan","contributor_surname":"Sun","orcid":""}],"concentration_method":null,"concentration_unit":null,"date":"2017-08-10","storage_temperature":null,"cell_temperature":16.0,"exposure_time":1.0,"number_of_frames":12,"wavelength":0.113,"sample_detector_distance":3.3,"concentration_min":0.15,"concentration_max":0.2,"sample_volume":null,"flow_rate":null,"s_min":0.043,"s_max":2.843,"total_exposure_time":null,"seccolumn":null},"fits":[{"models":[{"model_plot":"https://www.sasbdb.org/media/pdb_file/images/SASDFS3_fit1_model1_img.png","software":"GASBOR","pdb_link":[],"model_title":null,"type_of_model":"dummy","software_version":"2.3i","model_data":"https://www.sasbdb.org/media/pdb_file/SASDFS3_fit1_model1.pdb","model_mw":null,"bead_radius":1.9,"log":"https://www.sasbdb.org/media/log_files/RBCC_p2_b.log","symmetry":"p2","comment":null,"user":423}],"fit_unit":"1/A","fit_plot":"https://www.sasbdb.org/media/fitting_files/scattering_plots/RBCC_p2_b_fit_img.png","software":"GASBOR","chi_square_value":0.33,"p_value":0.012949,"fit_residual_plot":"RBCC_p2_b_fitresiduals_img.png","fit_data":"https://www.sasbdb.org/media/fitting_files/SASDFS3_fit1.fir","fit_log":"https://www.sasbdb.org/media/fitting_files/log_files/RBCC_p2_b.log","software_version":"2.3i","description":""}],"estimated_volume_method":null,"pddf_software":"ATSAS GNOM","pddf_software_version":"5.0","i0_calibration_standard":null,"description":null,"experiment_description":"Synchrotron SAXS data from solutions of TRIM28 RBCC (R184D mutant) in 10 mM Tris 300 mM NaCl 0.1 mM TCEP, pH 8 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 3.3 m and at a wavelength of λ = 0.113 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 16 °C using the following parameters: Column: GE-Healthcare S200 5/150 analytical SEC column; Flow rate: 0.5 mL/min; Sample injection concentration: 4.9 mg/mL; Injection volume: 50 μL. Total X-ray acquisition time = 690 s. The data were collected through the SEC peak of the protein as a series of 12 x 1 second exposures. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of an appropriate solvent-blank was subtracted.","tags":[],"intensity_unit":"1/cm","experimental_mw":70.0,"experimental_mw_error":null,"guinier_i0_mw":null,"guinier_i0_mw_error":null,"porod_mw":null,"porod_mw_error":null,"pddf_i0":0.0351,"pddf_i0_error":null,"guinier_i0":0.03473,"guinier_i0_error":0.00042,"pddf_rg":7.39,"pddf_rg_error":0.07,"guinier_rg":7.012,"guinier_rg_error":0.123,"pddf_dmax":23.2,"pddf_dmax_error":null,"porod_volume":232.0,"porod_volume_error":null,"estimated_volume":null,"estimated_volume_error":null,"guinier_point_first":4,"guinier_point_last":45,"pddf_point_first":null,"pddf_point_last":null,"i0_calibration_standard_data":null,"intensities_log_log_plot":"SASDFS3_datloglog_img.png","symmetry":null,"last_modified":"2019-12-06T13:07:15.624532+01:00","bragg_peak":[]}